超高效液相色谱-串联质谱法测定染发剂中9种染料

doi:10.13218/j.cnki.csdc.2017.05.012

摘要/Abstract

摘要： 建立了同时测定染发剂中9种染料的超高效液相色谱-串联质谱分析方法。样品用甲醇进行超声提取,以10 mmol/L 乙酸铵水溶液-乙腈为流动相,BEH-C₁₈色谱柱(2.1 mm×100 mm,1.7 μm)分离待测物,采用电喷雾离子源,正离子扫描和多反应监测(MRM)模式检测,外标法进行定量。9种染料羟乙基-2-硝基-p-甲苯胺、4-羟丙氨基-2-硝基苯酚、4-氨基-N,N-二甲基苯胺、3-硝基-p-羟乙氨基苯酚、2,6-二羟乙氨基甲苯、2-甲基-5-羟乙氨基苯酚、5-氨基-6-氯-2-甲基苯酚、2,6-二甲氧基-3,5-吡啶二胺盐酸盐和6-氨基间甲酚的检出限分别为1.7,0.84,0.27,3.4,0.034,1.2,7.0,0.17和0.20 mg/kg。样品加标回收实验的平均加标回收率为90.0%~110.0%,相对标准偏差为1.4%~8.6%(n=6)。该方法快速简便,定量准确,能够满足染发剂中染料含量的分析检测要求。

关键词: 染发剂, 染料, 超高效液相色谱-串联质谱

Abstract: A method for simultaneous determination of nine kinds of dyestuff in hair dyes by UPLC-MS/MS was established.The hair dye sample was extracted by methanol under assistance of ultrasonic irradiation.The separation was performed by a BEH-C₁₈ column (2.1 mm×100 mm,1.7 μm) with gradient elution of 10 mmol/L ammonium acetate and acetonitrile as mobile phase.The electrospray ionization (ESI) source with positive ion mode was used for the analysis of the nine components in the multiple reaction monitoring (MRM) mode.External standard method was used for quantification.The limit of detection of the nine kinds of dyestuff,including 2-(4-methyl-2-nitrophenylamino) ethanol,3-nitro-N-(2-hydroxyethyl)-4-aminophenol,4-amino-N,N-dimethylaniline,4-((2-hydroxypropyl) amino)-2-nitrophenol,bis-2,6-N,N-(2-hydroxyethyl) diaminotoluene,5-((2-hydroxyethyl) amino)-o-cresol,5-amino-6-chloro-2-methylphenol,3,5-diamino-2,6-dimethoxypyridine dihydrochloride and 6-amino-m-cresol are 1.7,0.84,0.27,3.4,0.034,1.2,7.0,0.17 and 0.20 mg/kg,respectively.The average recoveries of the nine kinds of dyestuff in the spiked samples are in the range of 90.0%-110.0% with the relative standard deviations (RSD,n=6) of 1.4%-8.6%.This method is simple,accurate and effective for simultaneous determination of the nine kinds of dyestuff in hair dyes.

Key words: hair dyes, dyestuff, ultra performance liquid chromatography-tandem mass spectrometry

中图分类号:

TQ658.3+4

戴明. 超高效液相色谱-串联质谱法测定染发剂中9种染料[J]. 日用化学工业, 2017, 47(5): 297-300.

DAI Ming. Simultaneous determination of nine kinds of dyestuff in hair dyes by ultra performance liquid chromatography-tandem mass spectrometry[J]. China Surfactant Detergent & Cosmetics, 2017, 47(5): 297-300.

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