欢迎访问《日用化学工业（中英文）》，今天是
日用化学工业（中英文）

日用化学工业 ›› 2015, Vol. 45 ›› Issue (9): 533-536.doi: 10.13218/j.cnki.csdc.2015.09.012

• 分析与检测 • 上一篇    

高效液相色谱法同时测定海娜染发粉中的散沫花醌和对苯二胺

吴艳苹1,2, 唐颖1, 宋文婷1, 曹蕊3, 刘洋4   

  1. 1.北京工商大学 理学院 化妆品系,北京 100048;
    2.北京市房山区食品药品安全监控中心,北京 102400;
    3.国家食品药品监督管理总局 保健食品审评中心,北京 100070;
    4.中国香料香精化妆品工业协会,北京 100079
  • 收稿日期:2015-06-24 修回日期:2015-08-27 出版日期:2015-09-22 发布日期:2019-04-10
  • 通讯作者: 唐 颖,博士,副教授,电话:(010)68984917,E-mail:tangying@th.btbu.edu.cn。
  • 作者简介:吴艳苹(1970-),女,北京人,硕士研究生,电话:(010)69313053。
  • 基金资助:
    国家自然科学基金资助项目(51403006); 北京市自然科学基金资助项目(2144046)

Simultaneous determination of lawsone and p-phenylenediamine in henna hair-coloring powders by high performance liquid chromatography

WU Yan-ping1,2, TANG Ying1, SONG Wen-ting1, CAO Rui3, LIU Yang4   


  1. 1.Department of Cosmetics,School of Science,Beijing Technology and Business University,Beijing 100048,China;
    2.Fangshan District Beijing Food and Drug Safety Monitoring Center,Beijing 102400,China;
    3.Center for Health Food Evaluation,China Food and Drug Administration,Beijing 100070,China;
    4.China Association of Fragrance Flavour and Cosmetic Industries,Beijing 100079,China
  • Received:2015-06-24 Revised:2015-08-27 Online:2015-09-22 Published:2019-04-10

PDF (PC)

369

赞

摘要： 建立了一种同时测定海娜染发粉中散沫花醌和对苯二胺的高效液相色谱法。样品经体积分数为50%的甲醇水溶液超声提取,采用Waters C18色谱柱和二极管阵列检测器,以V(甲醇)∶V(0.1 mol/L乙酸水溶液)=33.5∶66.5的混合溶液为流动相,流速1.0 mL/min,柱温30 ℃,检测波长280 nm。实验结果表明,散沫花醌和对苯二胺的检出限(S/N=3)分别为0.018和0.014 mg/L,定量限(S/N=10)分别为0.055和0.042 mg/L。当散沫花醌的质量浓度在0.515~51.50 mg/L、对苯二胺的质量浓度在21.50~430.00 mg/L范围内时,方法的线性关系良好,相关系数分别为0.999 8和0.999 4。对苯二胺的加标回收率(n=3)为102.20%~105.42%,RSD为0.36%~1.34%;散沫花醌的加标回收率为96.74%~107.30%,RSD为0.99%~2.30%。采用该方法对53份市售海娜染发粉样品进行检测:有30个样品只检出散沫花醌,12个样品同时检出散沫花醌和对苯二胺,7个样品只检出对苯二胺,4个样品散沫花醌和对苯二胺均未检出。

关键词: 海娜染发粉, 高效液相色谱法, 散沫花醌, 对苯二胺

Abstract: A high performance liquid chromatography (HPLC) method was established to detect lawsone and p-phenylenediamine in henna hair-coloring powder.The samples were dissolved by 50% methanol and analyzed by Waters C18 column (150 mm×4.6 mm,5 μm).Mobile phase is methanol and 0.1 mol/L acetic acid aqueous solution (volume ratio of 33.5∶66.5) at a flow rate of 1.0 mL/min.Operating temperature is 30 ℃.Detection wavelength is 280 nm.The detection limit (S/N=3) for lawsone and p-phenylenediamine is 0.018 and 0.014 mg/L respectively,and the limit of quantification (S/N=10) is 0.055 and 0.042 mg/L respectively.A good linear relation displays when lawsone is in the mass concentration range of 0.515-51.50 mg/L (r=0.999 8) and p-phenylenediamine is in the range of 21.50-430.00 mg/L (r=0.999 4).The spiked recoveries (n=3) are within the range of 102.20%-105.42% for p-phenylenediamine and 96.74%-107.30% for lawsone.The relative standard deviations (RSD) of p-phenylenediamine and lawsone are 0.36%-1.34% and 0.99%-2.30% respectively.Among test results of 53 henna hair-coloring powder samples from market,lawsone appears alone in 30 samples;both lawsone and p-phenylenediamine appear in 12 samples;p-phenylenediamine appears alone in seven samples and neither lawsone nor p-phenylenediamine appears in four samples.

Key words: henna hair-coloring powder, high performance liquid chromatography, lawsone, p-phenylenediamine

中图分类号: 

  • TQ658.3+4