松茸蛋白的制备、结构表征及其保湿功效研究

doi:10.3969/j.issn.2097-2806.2026.01.012

Abstract

Abstract:

Tricholoma matsutake is a rare fungus with medicinal and edible origin, and its protein has been relatively little studied. In order to investigate the moisturizing effect of matsutake proteins, in this study, total matsutake proteins （TMPS） were obtained from matsutake mycelia using hot water extraction and （NH₄）₂SO₄ salting out, and then a protein fraction （TMPS1-2） was obtained by purification by column chromatography with DEAE-52 and Sephadex G-100. The experimental results of Tricine-SDS-PAGE and infrared spectroscopy show that TMPS1-2 is a protein with a molecular weight of about 12.4 kDa, irregularly coiled, and with amino acids dominated by glutamic acid, proline and glycine. Mouse experiments show that TMPS1-2 can alleviate the phenomena of epidermal thickening, hyperkeratosis and water loss, enhance the expression of AQP3, and effectively improve the problem of skin dryness, with the efficacy of moisturizing and repairing the skin barrier.

Key words: Tricholoma matsutake, protein, moisturizing, skin barrier

CLC Number:

TQ658

Jiaming Li, Xiaofeng Qiu, Huishan Guo, Jiankun Luo, Lishe Gan, Xuetao Xu. Preparation, structural characterization and moisturizing efficacy of proteins from Tricholoma matsutake[J].China Surfactant Detergent & Cosmetics, 2026, 56(1): 98-106.

Figures/Tables 11

Tab.1

Fig.1

Fig.2

Tab.2

Fig.3

Fig.4

Fig.5

Fig.6

Fig.7

Fig.8

Fig.9

References 32

[1]	Draelos Z D. The science behind skin care: Moisturizers[J]. Journal of Cosmetic Dermatology, 2018, 17(2): 138-144. doi: 10.1111/jocd.12490 pmid: 29319217
[2]	邹鹏飞, 刘志河, 路万成, 等. 皮肤自身保湿系统和保湿护肤品设计思路[J]. 日用化学品科学, 2012, 35(1): 18-20.
[3]	Rawlings A V. Trends in stratum corneum research and the management of dry skin conditions[J]. International Journal of Cosmetic Science, 2003, 25(1-2): 63-95. doi: 10.1046/j.1467-2494.2003.00174.x pmid: 18494884
[4]	Denda M. Methodology to improve epidermal barrier homeostasis: how to accelerate the barrier recovery[J]. International Journal of Cosmetic Science, 2009, 31(2): 79-86. doi: 10.1111/j.1468-2494.2008.00487.x pmid: 19175430
[5]	李楚忠, 高红军, 丛琳. 天然植物保湿成分在护肤品中的应用概况[J]. 日用化学品科学, 2014, 37(7): 24-26.
[6]	葛麒, 朱春燕, 陈湖南, 等. 松茸蛋白抗氧化肽制备及构效关系研究[J]. 食品安全质量检测学报, 2023, 14(9): 9-17.
[7]	陈晓磊, 刘盼, 陈夫山. 麦麸多肽抗氧化及吸湿保湿性能的测定[J]. 齐鲁工业大学学报, 2018, 32(2): 8-12.
[8]	李玉珍, 肖怀秋, 刘淼, 等. 金属抗菌肽SIF₄基于胞内生物大分子靶点的大肠杆菌非膜损伤抑菌机理[J]. 食品科学, 2023, 44(21): 62-68. doi: 10.7506/spkx1002-6630-20221121-230
[9]	Trudell S A, Xu J, Saar I, et al. North American matsutake: names clarified and a new species described[J]. Mycologia, 2017, 109(3): 379-390. doi: 10.1080/00275514.2017.1326780 pmid: 28609221
[10]	Hou Y, Ding X, Hou W, et al. Structure elucidation and antitumor activity of a new polysaccharide from Maerkang Tricholoma matsutake[J]. International Journal of Biological Sciences, 2017, 13(7): 935-948. doi: 10.7150/ijbs.18953
[11]	Yin X L, You Q H, Jiang Z H. Immunomodulatory activities of different solvent extracts from Tricholoma matsutake (S. Ito et S. Imai) singer (higher basidiomycetes) on normal mice[J]. International Journal of Medicinal Mushrooms, 2012, 14(6): 549-56. doi: 10.1615/IntJMedMushr.v14.i6
[12]	Deng M G, Wang J, Li Y L, et al. Impact of polyphenols extracted from Tricholoma matsutake on UVB-induced photoaging in mouse skin[J]. Journal of Cosmetic Dermatology, 2021, 21(2): 781-793. doi: 10.1111/jocd.v21.2
[13]	Zhang Y J, Yang R, Wang L W, et al. Purification and characterization of a novel thermostable anticoagulant protein from medicinal leech Whitmania pigra Whitman[J]. Journal of Ethnopharmacology, 2022, 288: 114990. doi: 10.1016/j.jep.2022.114990
[14]	Chromy V, Svachova L, Novosad L, et al. Albumin-based or albumin-linked calibrators cause a positive bias in serum proteins assayed by the biuret method[J]. Clinical Chemistry and Laboratory Medicine, 2009, 47(1): 91-101. doi: 10.1515/CCLM.2009.011 pmid: 19117409
[15]	Hermann S. Tricine-SDS-PAGE[J]. Nature Protocols, 2006, 1(1): 16-22. doi: 10.1038/nprot.2006.4 pmid: 17406207
[16]	Jiang S Y, Liu S Q, Zhao C J, et al. Developing protocols of Tricine-SDS-PAGE for separation of polypeptides in the mass range 1-30 kDa with minigel electrophoresis system[J]. International Journal of Electrochemical Science, 2016, 11(1): 640-649. doi: 10.1016/S1452-3981(23)15870-6
[17]	Wang W L, Zhang L, Wang Z Q, et al. Physicochemical and sensory variables of Maillard reaction products obtained from Takifugu obscurus muscle hydrolysates[J]. Food Chemistry, 2019, 290: 40-46. doi: S0308-8146(19)30542-4 pmid: 31000054
[18]	Wang J, Jin W H, Hou Y, et al. Chemical composition and moisture-absorption/retention ability of polysaccharides extracted from five algae[J]. International Journal of Biological Macromolecules, 2013, 57: 26-29. doi: 10.1016/j.ijbiomac.2013.03.001 pmid: 23500437
[19]	Miyamoto T, Nojima H, Shinkado T, et al. Itch-associated response induced by experimental dry skin in mice[J]. The Japanese Journal of Pharmacology, 2002, 88(3): 285-292. doi: 10.1254/jjp.88.285
[20]	Wang B Y, Tai M L, Zhang K, et al. Elaeagnus L gum polysaccharides alleviate the impairment of barrier function in the dry skin model mice[J]. Journal of Cosmetic Dermatology, 2020, 20(2): 647-656. doi: 10.1111/jocd.v20.2
[21]	苏贞辉. 组织切片HE染色失败原因的查找和补救措施的研究[D]. 济南: 山东大学, 2016.
[22]	Bea H, Hoon S, Soojung O, et al. Mannosylerythritol lipids ameliorate ultraviolet A-induced aquaporin-3 downregulation by suppressing c-Jun N-terminal kinase phosphorylation in cultured human keratinocytes[J]. The Korean Journal of Physiology and Pharmacology, 2019, 23(2): 113-120. doi: 10.4196/kjpp.2019.23.2.113
[23]	Lang K, Xu K M, Dmitri B, et al. Arbutin encapsulated micelles improved transdermal delivery and suppression of cellular melanin production[J]. BMC Research Notes, 2016, 9(1): 254-256. doi: 10.1186/s13104-016-2047-x
[24]	赖雨微, 张倩, 高俊鹏, 等. 响应面法优化松茸蛋白的提取工艺[J]. 中国调味品, 2022, 47(8): 90-95, 105.
[25]	宁永成. 有机化合物结构鉴定与有机波谱学[M]. 北京: 科学出版社, 2018: 286-290.
[26]	Jung C. Insight into protein structure and protein-ligand recognition by Fourier transform infrared spectroscopy[J]. Journal of Molecular Recognition, 2000, 13(6): 325-351. doi: 10.1002/1099-1352(200011/12)13:6<325::AID-JMR507>3.0.CO;2-C pmid: 11114067
[27]	András M, Éva M, Nikoletta M, et al. Disordered-ordered protein binary classification by circular dichroism spectroscopy[J]. Frontiers in Molecular Biosciences, 2022, 9: 863141. doi: 10.3389/fmolb.2022.863141
[28]	Denda M, Sato J, Masuda Y, et al. Exposure to a dry environment enhances epidermal permeability barrier function[J]. Journal of Investigative Dermatology, 1998, 111(5): 858-863. pmid: 9804350
[29]	金锦花, 李科岩, 王玉慧, 等. 皮肤干燥症瘙痒小鼠模型的建立[J]. 中华实用诊断与治疗杂志, 2020, 34(3): 246-249.
[30]	Siya K, Sheela A, et al. Physiological, pathological, and circadian factors impacting skin hydration[J]. Cureus, 2022, 14(8): 27666.
[31]	Yuswan H M, Jalil A H N, Mohamad H, et al. Hydroxyproline determination for initial detection of halal-critical food ingredients (gelatin and collagen)[J]. Food Chemistry, 2021, 337: 127762. doi: 10.1016/j.foodchem.2020.127762
[32]	Wendy B B, Lorry A, Joseph W, et al. Aquaporin-3 in the epidermis: more than skin deep[J]. American Journal of Physiology-cell Physiology, 2020, 318(6): 1144-1153. doi: 10.1152/ajpcell.00075.2020 pmid: 32267715

	16.5%分离胶	10%夹层胶	4%浓缩胶
V（AB-3）/mL	-	0.407	0.16
V（AB-6）/mL	3	-	-
V（GB）/mL	3	0.667	0.496
V（50%甘油）/mL	1.92	-	-
V（ddH₂O）/mL	1.08	0.925	1.25
V（10% APS）/μL	80	20	20
V（TEMED）/μL	10	5.2	5

氨基酸种类	w（TMPS）/%	w（TMPS1-2）/%
酪氨酸	0.33	0.44
精氨酸	5.13	7.37
苯丙氨酸^*	1.28	1.93
组氨酸	0.79	0.93
蛋氨酸	0.38	0.43
甘氨酸	7.88	10.68
赖氨酸^*	2.67	3.61
天冬氨酸	4.32	5.89
异亮氨酸^*	1.19	1.52
亮氨酸^*	1.73	3.41
苏氨酸^*	1.35	2.77
缬氨酸^*	1.86	2.43
脯氨酸	9.59	13.00
丝氨酸	2.31	3.11
丙氨酸	6.31	8.97
谷氨酸	16.00	23.78

Preparation, structural characterization and moisturizing efficacy of proteins from Tricholoma matsutake

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 11

References 32

Related Articles 15

Metrics

Comments

Recommended 0

[1]	Siyue Xie, Can Qin, Junmin Lin, Xiaochun Luo. The activity and skin-caring efficacy of amino acids/peptides fermented from meal protein [J]. China Surfactant Detergent & Cosmetics, 2025, 55(9): 1187-1197.
[2]	Yutong Liu, Bingbing Yuan, Tiantian Han, Gong Chen, Yonghang Mu, Linhua Song, Zi Wang. Interaction mechanism and application of the aqueous mixtures of surfactants and proteins [J]. China Surfactant Detergent & Cosmetics, 2025, 55(9): 1198-1206.
[3]	Sijia Yang, Hui Liu, Yongbo Lyu, Yuxin Huang, Shujing Li. Preparation and efficacy study of dendrobium polysaccharide/purulan nanofiber solid absorbable mask [J]. China Surfactant Detergent & Cosmetics, 2025, 55(7): 879-886.
[4]	Fan Wu, Jiaqi Zhang, Yi Qin, Jun Wang, Zonghan Wu, Yao Pan. Progress of cosmetic active ingredients exerting skincare efficacy through cellular autophagy [J]. China Surfactant Detergent & Cosmetics, 2025, 55(6): 803-810.
[5]	Xingting Wang, Zhaojuan Shi, Jun Wu, Chuanxun Yuan, Risheng Jin. Preparation and study of Camellia oleifera seed oil-ceramide nanoemulsion [J]. China Surfactant Detergent & Cosmetics, 2025, 55(6): 739-746.
[6]	Shiya Xie, Mingwei Liu, Lei Xiao, Shaolin Hu. Study on the antibacterial activity and anti-inflammatory activity of dandelion polyphenols [J]. China Surfactant Detergent & Cosmetics, 2025, 55(5): 633-639.
[7]	Wucheng Yang, Yu Xie, Jian Wei, Ruifang Fan, Jianhua Tan, Shaofeng Xi. Application of confocal Raman spectroscopy in the evaluation of skin barrier function and permeability [J]. China Surfactant Detergent & Cosmetics, 2025, 55(4): 508-515.
[8]	Huabing Zhao, Yingtian Li, Xihan Wang, Zhengmei Huang, Fuping Lu. Skin microecology and microecological skincare products [J]. China Surfactant Detergent & Cosmetics, 2025, 55(3): 390-398.
[9]	Ya Zhang, Hao Zhou, Rong Wang, Zengmiao Hou, Wentao Huang, Lu Yang. Characterization of recombinant elastin and its roles in ultraviolet-induced skin photoaging [J]. China Surfactant Detergent & Cosmetics, 2024, 54(9): 1106-1116.
[10]	Shengpeng Li, Jing Li, Chao Liang, Ran Zhao, Xiaojie Zhang, Lili Sun. Protective effect of silk fibroin on UVB-induced skin injury [J]. China Surfactant Detergent & Cosmetics, 2024, 54(9): 1117-1124.
[11]	Zhaoyi Liu, Xinyu Chen, Yan Wang, Xue Li, Ruoxi Guo, Han Zhang. Study on the reparative effects of oxymatrine on impaired skin barrier function in mice [J]. China Surfactant Detergent & Cosmetics, 2024, 54(7): 777-783.
[12]	Hui Liu, Sijia Yang, Hankun Ren, Zhaohui Qu, Libo Zheng, Shujing Li. Research progress of extraction and isolation of natural plant polysaccharides and their applications in skin care [J]. China Surfactant Detergent & Cosmetics, 2024, 54(6): 708-717.
[13]	Guo Fangyu, Han Tingting, Wang Xiaona, Chen Yurong, Wang Xiaomei, Yang Suzhen. Process optimization and anti-aging, moisturizing and whitening effects of traditional Chinese medicine bi-directional fermentation broth [J]. China Surfactant Detergent & Cosmetics, 2023, 53(5): 523-531.
[14]	Fan Jiayi, Ma Ling, Chen Timson, Chang Kuan, Wang Jing. Scientific foundations of hair and scalp care （Ⅳ） Development of protein and polypeptide ingredients for hair care [J]. China Surfactant Detergent & Cosmetics, 2023, 53(4): 382-389.
[15]	Li Huiling, Zhou Chunxia, Zhang Zhang. Study on skin care effects of Rosa graciliflora callus extract in human dermal fibroblast cells and 3D epidermal model [J]. China Surfactant Detergent & Cosmetics, 2023, 53(3): 300-307.