雪菊与芦荟醇提物对UVB导致的小鼠皮肤光损伤的保护作用

doi:10.3969/j.issn.2097-2806.2024.11.008

Abstract

Abstract:

Snow chrysanthemum and Aloe vera have great potential to protect the skin from photodamage. In this paper, we investigated the protective effects of ethanolic extracts of snow chrysanthemum and Aloe vera against outdoor UVB-induced skin photodamage in mice. According to the experimental requirements, the mice were divided into a normal control group （NC group）, a model control group （MC group）, a snow chrysanthemum alcoholic extract group （SCAE group）, a Aloe vera alcoholic extract group （AVAE group）, and a snow chrysanthemum and Aloe vera alcoholic extract group （SCAE+AVAE group）. In order to reveal the protective mechanism of alcoholic extract against photodamage in mouse skin, antioxidant indexes, hydroxyproline （HYP） content and serum-associated inflammatory factors in damaged skin were determined in mice. The results show that all three intervention groups significantly improve UVB-induced skin appearance in mice compared to the MC group. By hematoxylin-eosin staining （HE）, it indicates that the SCAE+AVAE and SCAE are more effective than the AVAE, and the mice have healthier and more elastic skin. The three intervention groups also reduce UVB-induced oxidative stress damage in mice skin, and the malondialdehyde （MDA） content of the SCAE+AVAE group is only （28.18±4.25） nmol/mgprot, which is significantly lower than that of the other groups. The activities of three antioxidant enzymes: catalase （CAT）, superoxide dismutase （SOD）, and glutathione peroxidase （GSH-Px） are also significantly higher in the SCAE+AVAE group. The SCAE+AVAE group is more effective than the alcoholic extract alone group, and the HYP content reaches （4.12±0.18） μg/mg. All three intervention groups can down-regulate the content of inflammatory factors and inhibit the inflammatory response, among which the SCAE+AVAE group is more effective. And the application of SCAE+AVAE reduces the activities of interleukin-1 （IL-1）, interleukin-6 （IL-6） and tumour necrosis factor-α （TNF-α） by 38.93%, 43.31% and 46.34%, respectively. Notably, the effect of the combined extracts is much greater than that of the extracts singly at the same dose. This study reveals the synergistic protective effect of SCAE+AVAE in the treatment of UV-induced photodamage, which is promising for skin care and cosmetic applications.

Key words: snow chrysanthemum, Aloe vera, UVB irradiation, skin photodamage, synergistic effect

CLC Number:

TQ658

Yang Li, Youhang Shi, Qingyao Lv, Hongyan Jiao, Shirong Jiao. Protective effect of snow chrysanthemum and Aloe vera alcoholic extract on photodamage of skin in mice caused by UVB[J].China Surfactant Detergent & Cosmetics, 2024, 54(11): 1347-1354.

Figures/Tables 7

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References 26

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	体重/g
	D0	D3	D5	D7	D9	D11	D14
NC组	27.61±1.35	29.48±0.36	32.27±0.37	33.82±0.57	34.43±0.28	35.78±0.41	36.24±0.51
MC组	27.79±1.65	28.29±0.34^##	29.66±0.75^##	30.75±0.54^##	32.06±0.69^##	32.99±0.64^##	33.80±0.47^##
SCAE组	27.51±1.57	29.79±0.85^**	31.61±0.35^**	32.91±0.51^**	33.72±0.59^**	34.75±0.82^**	35.68±0.33^**
AVAE组	27.72±1.11	29.24±1.32^**	30.87±1.60^**	32.32±1.13^**	33.21±0.47^**	34.27±1.45^**	35.15±0.91^**
SCAE+AVAE组	27.80±1.70	29.99±0.58^**	32.84±0.42^**	34.26±0.65^**	35.38±0.56^**	36.32±0.57^**	36.76±0.52^**

	IL-1含量/（pg/mL）	IL-6含量/（pg/mL）	TNF-α含量/（pg/mL）
NC组	133.71±17.20^a	44.37±10.13^a	178.69±19.35^a
MC组	225.71±21.38^c	92.65±9.72^c	396.13±45.33^e
SCAE组	150.25±12.33^a	70.07±12.47^b	251.68±13.92^c
AVAE组	170.45±18.79^b	65.06±5.60^b	284.18±19.57^d
SCAE+AVAE组	137.83±18.94^a	52.52±8.11^a	212.57±12.86^b

Protective effect of snow chrysanthemum and Aloe vera alcoholic extract on photodamage of skin in mice caused by UVB

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