依克多因与羟基积雪草甙协同抗炎作用的研究

doi:10.3969/j.issn.1001-1803.2021.06.010

Abstract

Abstract:

The inflammation model of RAW264.7 cells induced by LPS was used to detect that Ectoin, Madecassoside and their combination under different experimental set mass concentrations and ratios had good inhibitory effect on the production of NO in LPS-induced RAW264.7 cells, that is, it had anti-inflammatory activity at the cellular level. Meanwhile, under different experimental set mass concentrations and ratios, most of the combination of Ectoin and Madecassoside show higher inhibition rate of NO than that of a single component. In addition, the SI values under different experimental set mass concentrations and ratios are lower than 1. The above results demonstrate that the combination of Ectoin and Madecassoside has a synergistic interaction in anti-inflammatory, however, the mechanism needs further study.

Key words: Ectoin, Madecassoside, anti-inflammatory, synergistic effect

CLC Number:

TQ658

ZHOU Hu-wu,HUO Yong-li,FANG Li,MA Shi-jing,HAN Ping,LIN Li,DU Zhi-yun. Studies on the synergistic anti-inflammatory effects of Ectoin and Madecassoside[J].China Surfactant Detergent & Cosmetics, 2021, 51(6): 535-538.

Figures/Tables 6

Tab. 1

Fig. 1

Tab. 2

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Fig. 2

Tab. 4

References 14

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质量浓度/（μg·mL^-1）	RAW264.7巨噬细胞活力/%
质量浓度/（μg·mL^-1）	依克多因（EC）	羟基积雪草甙（MC）
100	100.47±0.65	95.68±0.93
50	108.88±0.62	109.47±1.05
5	105.07±0.87	107.43±1.17
1	98.31±0.44	98.00±1.04
0.1	98.86±1.14	100.98±0.97

实验组	NO含量/（μmol·L^-1）
实验组	20 μg/mL	10 μg/mL	5 μg/mL
空白组	32.29±0.83	—	—
模型组	61.06±1.76	—	—
依克多因（EC）	59.37±0.30	57.09±0.18	56.09±0.44
羟基积雪草甙（MC）	59.13±0.10	52.35±0.30	50.30±0.71
MC+EC（1:1）	59.31±1.17	51.82±1.37	49.95±0.10
MC+EC（1:3）	55.33±0.70	54.40±0.66	55.51±0.18
MC+EC（1:9）	49.19±1.99	48.08±0.41	51.36±0.10
MC+EC（1:19）	48.61±0.44	48.73±0.27	50.01±0.10
MC+EC（1:99）	58.84±0.30	50.25±1.10	46.04±0.18

实验组	NO抑制率/%
实验组	20 μg/mL	10 μg/mL	5 μg/mL
模型组	—	—	—
依克多因（EC）	2.83	10.19	13.40
羟基积雪草甙（MC）	3.58	25.47	32.08
MC+EC（1:1）	6.98	31.30	38.75
MC+EC（1:3）	19.11	22.63	19.51
MC+EC（1:9）	40.24	45.39	33.88
MC+EC（1:19）	43.36	43.22	38.48
MC+EC（1:99）	5.20	32.53	45.91

实验组	协同指数			协同判定（0<SI<1为协同增效）
实验组	20 μg/mL	10 μg/mL	5 μg/mL	协同判定（0<SI<1为协同增效）
MC+EC（1:1）	0.459 6	0.569 6	0.586 7	√
MC+EC（1:3）	0.158 0	0.619 1	0.925 9	√
MC+EC（1:9）	0.072 2	0.258 1	0.450 6	√
MC+EC（1:19）	0.066 1	0.253 4	0.372 4	√
MC+EC（1:99）	0.545 3	0.317 9	0.295 9	√

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Studies on the synergistic anti-inflammatory effects of Ectoin and Madecassoside

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