重组贻贝粘蛋白改善雄激素脱发效果的体外评价及 机制研究

doi:10.3969/j.issn.2097-2806.2026.04.010

Abstract

Abstract:

The study aims to investigate the effects and mechanisms of recombinant mussel adhesive protein on improving androgenetic alopecia （AGA） through in vitro experiments. The recombinant mussel adhesive protein was structurally characterized through molecular weight and protein content analysis. The effects of different mass concentrations of recombinant mussel mucin on type II 5α-reductase inhibition, human dermal papilla cell （HDPCs） viability were examined, and in the DHT-induced AGA cell model, the changes in inflammation factors, growth regulatory factors （VEGF and HGF） secretion, reactive oxygen species （ROS） levels, and cellular senescence marker β-galactosidase （SA-β-gal） activity were assessed, verifying its anti-androgenetic alopecia efficacy from multiple dimensions. The results show that the measured molecular weight of recombinant mussel adhesive protein （22 681.366 Da） is highly consistent with the theoretical value, and the purity of three batches of raw materials all exceeds 90%, which ensures the reliability of subsequent functional studies. Functional studies on recombinant mussel adhesive protein reveal that it exhibits an inhibitory effect on type II 5α-reductase （IC₅₀ = 1 500 μg/mL） with a dose-dependent relationship. Furthermore, regarding its effect on HDPCs cell, recombinant mussel adhesive protein can promote the proliferation of HDPCs in a concentration-dependent manner without toxicity at high concentrations. Further studies indicate that recombinant mussel adhesive protein exerts a hair growth-promoting effect by inhibiting DHT-induced ROS production, reducing the release of pro-inflammatory factors such as IL-6 and TNF-α, while significantly decreasing the expression of SA-β-gal to delay the premature senescence of HDPCs and maintain their proliferation potential. Notably, the efficacy of recombinant mussel adhesive protein at low concentrations is comparable to that of minoxidil.

Key words: androgenic alopecia, type II 5α-reductase, dermal papilla cells, premature senescence, hair follicle regulation

CLC Number:

TQ658

Qiu Zhang, Ya Zhang, Rong Wang, Zengmiao Hou, Wentao Huang, Lu Yang. Evaluation of the efficacy of recombinant mussel adhesive protein in improving androgenetic alopecia and study on its mechanism in vitro[J].China Surfactant Detergent & Cosmetics, 2026, 56(4): 497-504.

Figures/Tables 7

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References 21

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批号	样品称样量/mg	消耗硫酸用量/mL	测得蛋白含量/mg	含量/ %	平均含量/%	RSD/ %
2309Y01	10.83	9.855 0	10.10	93.30	93.42	0.30
	10.36	9.420 0	9.66	93.23
	10.45	9.554 2	9.80	93.74
2309Y02	10.24	9.357 0	9.59	93.69	93.51	0.24
	10.52	9.602 0	9.84	93.58
	10.43	9.487 1	9.73	93.26
2309Y03	10.22	9.332 6	9.57	93.63	93.81	0.32
	10.34	9.495 2	9.74	94.15
	10.51	9.598 9	9.84	93.64

ρ （重组贻贝粘蛋白） /（μg/mL）	II型5α-还原酶抑制率/ %	IC₅₀/ （μg/mL）
200	16.11±1.10	1 500
400	17.09±0.98
800	35.26±2.18
1 000	42.05±2.44
2 000	58.33±3.27

ρ （重组贻贝粘蛋白） / （μg/mL）	相对增殖率/%
ρ （重组贻贝粘蛋白） / （μg/mL）	24 h	48 h	72 h
对照	100.00±0.00	100.00±0.00	100.00±000
10	103.34±4.02	110.01±3.79^*	116.48±4.96^*
30	131.55±2.85^**	118.67±3.89^*	114.16±9.27^*
50	125.96±2.54^**	112.94±8.32^*	105.15±8.69
100	117.09±1.67^*	110.06±9.01^*	104.07±10.82
150	110.63±4.27^*	109.92±7.71^*	105.08±11.50

	HDPCs细胞活力/%
对照	100.00±0.00
DHT	73.21±6.50^#
DHT + 2 000 μg/mL米诺地尔	85.00±3.66^*
DHT + 10 μg/mL重组贻贝粘蛋白	92.52±10.68^**
DHT + 30 μg/mL 重组贻贝粘蛋白	93.00±14.53^**
DHT + 50 μg/mL 重组贻贝粘蛋白	81.92±6.11^*

Evaluation of the efficacy of recombinant mussel adhesive protein in improving androgenetic alopecia and study on its mechanism in vitro

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