油茶籽油-神经酰胺纳米乳液制备及研究

doi:10.3969/j.issn.2097-2806.2025.06.008

Abstract

Abstract:

In order to enhance the development and application of Camellia oleifera seed oil （CSO） in the field of cosmetics, the study utilizes CSO as the cosmetic base oil and combines it with ceramide （Cer）. High-pressure homogenization was employed to prepare and optimize the best formula and conditions for the CSO-Cer nanoemulsion. The results show that under the conditions of homogenization pressure at 100 MPa, 7 cycles, a CSO mass fraction of 15%, a Cer addition of 1%, a hydrogenated lecithin mass fraction of 4%, a cholesterol mass fraction of 0.2%, and a glycerol mass fraction of 25%, a nanoemulsion with optimal particle size and stability is obtained. This emulsion has a particle size of 124.7 nm, a polydispersity index of 0.108, and a Zeta potential of -55.7 mV. Further testing reveals that the CSO-Cer nano-emulsion exhibits excellent moisturizing and transdermal properties. Consequently, the CSO-based CSO-Cer nanoemulsion is a potential new type of functional cosmetic emulsion, providing significant technical reference for the subsequent application of CSO in the field of cosmetics.

Key words: Camellia oleifera seed oil, ceramide, nanoemulsion, moisturizing, transdermal

CLC Number:

TQ658

Xingting Wang, Zhaojuan Shi, Jun Wu, Chuanxun Yuan, Risheng Jin. Preparation and study of Camellia oleifera seed oil-ceramide nanoemulsion[J].China Surfactant Detergent & Cosmetics, 2025, 55(6): 739-746.

Figures/Tables 12

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References 31

[1]	Fang Ziyang, Li Guizhen, Yao Gu, et al. Flavour analysis of different varieties of Camellia seed oil and the effect of the refining process on flavour substances[J]. Food Science and Technology, 2022, 170: 114040.
[2]	Wang L, Zhang Y, Chen J F, et al. Study on preparation and properties of Camellia oleifera seed oil microcapsules by complex coacervation and spray drying[J]. Food Science and Technology, 2023, 184: 115056.
[3]	Song F, Li Y, Wang B, et al. Effect of drying method and wall material composition on the characteristics of Camellia seed oil microcapsule powder[J]. Journal of the American Oil Chemists’ Society, 2021, 99 (4) : 353-364.
[4]	Zhang H, Han M, Nie X, et al. Production of Camellia oleifera Abel seed oil for injection: extraction, analysis, deacidification, decolorization, and deodorization[J]. Foods, 2024, 13 (10) : 1430.
[5]	Kim A R, Lee N H, Park Y M, et al. Preparation and characterization of novel pseudo ceramide liposomes for the transdermal delivery of baicalein[J]. Journal of Drug Delivery Science and Technology, 2019, 52: 150-156.
[6]	Xu F, Zhu J, Zhang Z, et al. In vivo evaluation of the skin penetration and efficacy of ceramide nanomulsions by confocal Raman spectroscopy[J]. Journal of Raman Spectroscopy, 2023, 54 (12) : 1408-1415.
[7]	Su R, Yang L, Wang Y, et al. Formulation, development, and optimization of a novel octyldodecanol-based nanoemulsion for transdermal delivery of ceramide IIIB[J]. International Journal of Nanomedicine, 2017, 12: 5203-5221. doi: 10.2147/IJN.S139975 pmid: 28860748
[8]	Shin K, Lee K B, Hwang J H, et al. Multilamellar ceramide core-structured microvehicles with substantial skin barrier function recovery[J]. Journal of Materials Chemistry B, 2023, 11 (10) : 2135-2144. doi: 10.1039/d2tb02734h pmid: 36762491
[9]	Kahraman E, Kaykn M, Şahin Bektay H, et al. Recent advances on topical application of ceramides to restore barrier function of skin[J]. Cosmetics, 2019, 6 (3) : 52.
[10]	Meckfessel L M H, Brandt S. The structure, function, and importance of ceramides in skin and their use as therapeutic agents in skin-care products[J]. Journal of the American Academy of Dermatology, 2014, 71 (1) : 177-184. doi: 10.1016/j.jaad.2014.01.891 pmid: 24656726
[11]	Sakae H, Oglso Y, Matsuda A M, et al. Ceramide nanoliposomes as potential therapeutic reagents for asthma[J]. Cells, 2023, 12 (4) : 591.
[12]	Hatziantoniou S, Deli G, Nikas Y, et al. Scanning electron microscopy study on nanoemulsions and solid lipid nanoparticles containing high amounts of ceramides[J]. Micron, 2007, 38 (8) : 819-823. pmid: 17693092
[13]	Bansal R, Pandey K K. Surface protection of wood using cerium oxide nanoparticles dispersed paraffin wax nanoemulsion[J]. Materials Chemistry and Physics, 2023, 306: 128042.
[14]	Martinez R, Bhanawat A, Yalcin R A, et al. Rigorous accounting for dependent scattering in thick and concentrated nanoemulsions[J]. The Journal of Physical Chemistry C, 2024, 128 (15) : 6419-6430.
[15]	Hu Y Q, Yin S W, Zhu J H, et al. Fabrication and characterization of novel Pickering emulsions and Pickering high internal emulsions stabilized by gliadin colloidal particles[J]. Food Hydrocolloids, 2016, 61: 300-310.
[16]	Lee N H, Park S H, Park S N. Preparation and characterization of novel pseudo ceramide-based nanostructured lipid carriers for transdermal delivery of apigenin[J]. Journal of Drug Delivery Science and Technology, 2018, 48: 245-252.
[17]	缪晓琴, 金抒. 反相高效液相色谱法测定化妆品中的神经酰胺[J]. 日用化学工业, 2014, 44 (2) : 112-114.
[18]	王轲. 氢化卵磷脂形成液晶的机理及其应用性能研究[D]. 上海: 上海应用技术大学, 2018.
[19]	Wu M, He X, Feng D, et al. The effect of high pressure homogenization on the structure of dual-protein and its emulsion functional properties[J]. Foods, 2023, 12 (18) : 3358.
[20]	Rave M C, Echeverri J D, Salamanca C H. Improvement of the physical stability of oil-in-water nanoemulsions elaborated with sacha inchi oil employing ultra-high-pressure homogenization[J]. Journal of Food Engineering, 2020, 273: 109801.
[21]	王玙璇, 梁蓉, 骆晔媛. 神经酰胺纳米乳液的制备及透皮性能研究[J]. 日用化学工业, 2021, 51 (10) : 949-959.
[22]	孙亚欣, 郑晓燕, 郑丽丽, 等. 大豆分离蛋白-茶皂素复合乳化剂制备山茶油纳米乳液及其性质研究[J]. 食品工业科技, 2020, 41 (22) : 27-34, 42.
[23]	Marc A L, Douglas A B, Jamey P W, et al. Formulation of polyiodinated triglyceride analogues in a chylomicron remnant-like liver-selective delivery vehicle[J]. Pharmaceutical Research, 1996, 13 (6) : 875-879. pmid: 8792425
[24]	Yun S, Kim G W, Jang J, et al. Ensuring long-term stability and size control of nanoemulsion via post-microfluidization dilution troward energy saving scale-up process[J]. Colloids and Surface, 2024, 691: 133845.
[25]	Paulo L A D O, Fernandes R N, Simiqueli A A, et al. Baru oil applied in the formation of O/W nanoemulsions: A study of physical-chemical, rheological and interfacial properties[J]. Food Research International, 2023, 170: 112961.
[26]	Inapurapu S P, IbrahimA, Kona S R, et al. Development and characterization of ω-3 fatty acid nanoemulsions with improved physicochemical stability and bioaccessibility[J]. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2020, 606: 125515.
[27]	Zhou L, Peng Y, Xu Z, et al. The antioxidant, anti-inflammatory and moisturizing effects of Camellia oleifera oil and its potential applications[J]. Molecules, 2024, 29 (8) : 1864.
[28]	Takeda S, Yoneda A, Miyasaka K, et al. Comparative study on epidermal moisturizing effects and hydration mechanisms of rice-derived glucosylceramides and ceramides[J]. International Journal of Molecular Sciences, 2022, 24 (1) : 83.
[29]	赵冰怡, 杨洪芹, 潘启诚, 等. 神经酰胺复合物在配方中的保湿修护功效评价[J]. 中国洗涤用品工业, 2022 (8) : 70-77.
[30]	Engelbrecht T N, SchroeterA, Hauβ T, et al. Lipophilic penetration enhancers and their impact to the bilayer structure of stratum corneum lipid model membranes: Neutron diffraction studies based on the example oleic acid[J]. Biochimica et Biophysica Acta, 2011, 1808 (12) : 2798-2806. doi: 10.1016/j.bbamem.2011.08.012 pmid: 21871866
[31]	Lo W, Ghazaryan A, Tso C H, et al. Oleic acid-enhanced transdermal delivery pathways of fluorescent nanoparticles[J]. Applied Physics Letters, 2012, 100 (21) : 213701.

试验号	A	B	C	粒径/nm
1	1	1	1	163.9
2	1	2	2	161.8
3	1	3	3	178.7
4	2	1	2	132.8
5	2	2	3	144.6
6	2	3	1	126.2
7	3	1	3	191.3
8	3	2	1	166.9
9	3	3	2	172.8
R₁	168.133	162.667	152.233
R₂	134.533	157.767	155.588
R₃	177.000	159.233	171.533
R	42.467	4.900	19.300

方差来源	离差平方和	自由度	均方	F值	P值	显著性
A	3 011.00	2	1 505.50	2 049.85	0.000 5	**
B	37.95	2	18.97	25.84	0.037 3	*
C	628.20	2	314.10	427.67	0.002 3	**
误差	1.47	2	0.734 4

Preparation and study of Camellia oleifera seed oil-ceramide nanoemulsion

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水平	因素
水平	w（油茶籽油）/% （A）	w（胆固醇）/% （B）	w（甘油）/% （C）
1	10	0	25
2	15	0.2	35
3	20	0.4	45

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