复方丁香挥发油对UV致小鼠皮肤光老化损伤的保护作用

doi:10.3969/j.issn.2097-2806.2023.12.004

Abstract

Abstract:

Photoaging is a process of premature skin aging caused by prolonged exposure to ultraviolet radiation. It is characterized by sunspots, coarse wrinkles, loss of elasticity, and photochemical keratosis. The protective effect of volatile oil of compound clove on the skin of mice induced by UV was studied in this paper. Firstly, the main components of volatile oil of compound clove were analyzed by gas chromatography-mass spectrometry （GC-MS）. After the back irradiation, the skin tissues of the irradiated area were stained by HE and Masson to observe the changes of the skin morphology and collagen fibers in the dermis. The expressions of collagen Ⅰ and MMP-3 in skin were detected by Western Blot. The results show that the volatile oil of compound clove contains 50 components, of which 11 components account for 83.56% of the chromatographic peak area. The main components are eugenol （31.96%）, （E）-3-butylidene-4, 5-dihydroisobenzofuran-1（3H）-one （20.56%） and caffeine （12.72%）. Compared with the model group, compound clove volatile oil significantly improves the appearance and tissue structure of the photoaged mice skin, and increases collagen fibers. The results of Western Blot show that compound clove volatile oil can up-regulate the expression of typeⅠcollagen and down-regulate the expression of MMP-3 protein. In conclusion, compound clove volatile oil can improve skin wrinkles and roughness caused by ultraviolet light, inhibit collagen degradation in photoaged skin, and promote skin repair.

Key words: compound clove volatile oil, GC-MS, chemical components, photoaging

CLC Number:

TQ658

Yu Ding, Yi Wu, Mingshi Ren, Zihan Li, Bo Liu. Protective effect of compound clove volatile oil on UV induced skin photoaging damage in mice[J].China Surfactant Detergent & Cosmetics, 2023, 53(12): 1385-1391.

Figures/Tables 8

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References 24

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等级	皮肤外观
0	正常皮肤纹理，肉眼可见正常细小皮纹，无皱纹
1	可见少量横向浅皱纹
2	浅皱纹消失，部分深皱纹存在
3	浅皱纹完全消失，深皱纹严重并持续存在
4	深皱纹严重并持续存在且皮肤泛红

化合物名称	保留时间/min	m/z	峰面积	百分比/%	A/H	CAS号
Eugenol	19.644	TIC	75 690 783	31.96	3.45	97-53-0
（E）-3-Butylidene-4, 5-dihydroisobenzofuran-1（3H）-one	28.810	TIC	48 690 348	20.56	3.38	81944-8-3
Caffeine	31.123	TIC	30 122 114	12.72	3.60	58-08-2
Epicurzerenone	25.823	TIC	11 281 313	4.76	2.73	20085-85-2
Caryophyllene	21.262	TIC	7 215 729	3.05	2.83	87-44-5
Phenol, 2-methoxy-4-（2-propenyl）-, acetate	23.924	TIC	6 952 180	2.94	2.66	93-28-7
1, 1, 7, 7a-Tetramethyl-1a, 2, 6, 7, 7a, 7b-hexahydro-1H-cyclopropa[a]naphthalene	28.956	TIC	4 789 442	2.02	3.39	154098-14-3
Isospathulenol	26.378	TIC	3 951 159	1.67	2.90	88395-46-4
Cyclohexene, 4-ethenyl-4-methyl-3-（1-methylethenyl）-1-（1-methylethyl）-, （3R-trans）-	19.049	TIC	3 288 362	1.39	2.65	20307-84-0
Cyclohexane, 1-ethenyl-1-methyl-2, 4-bis（1-methylethenyl）-, [1S-（1.alpha., 2.beta., 4.beta.）]-	20.518	TIC	3 283 270	1.39	2.55	515-13-9
Bicyclo[5.3.0]decane, 2-methylene-5-（1-methylvinyl）-8-methyl-	23.028	TIC	2 602 024	1.10	2.85	0-0-0
Copaene	20.101	TIC	2 361 069	1.00	2.58	3856-25-5

组别	皮肤纹理评分/分
正常组	—
模型组	3.42±0.49^**
大豆油组	3.25±0.43
复方丁香挥发油低剂量组	1.80±0.60^##
复方丁香挥发油中剂量组	1.50±0.76^##
复方丁香挥发油高剂量组	3.17±0.55
V_E组	2.00±0.82^##

Protective effect of compound clove volatile oil on UV induced skin photoaging damage in mice

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