多季铵盐与鲑鱼精DNA的相互作用研究

doi:10.13218/j.cnki.csdc.2016.08.001

Abstract

Abstract: The interaction between the multi-quaternary ammonium salt (MQAS) and salmon sperm DNA was investigated using UV-vis absorption spectroscopy,fluorescence spectroscopy,circular dichroism spectroscopy and molecular simulation.The results showed that MQAS binds to salmon sperm DNA by static electricity,and the binding ratio is 5.5∶1.The presence of salmon sperm DNA promotes the formation of MQAS aggregates.With decreasing chain length,the interaction between MQAS and salmon sperm DNA is stronger.In addition,MQAS causes the conformational change of salmon sperm DNA.The results of molecular simulation suggested that MQAS inserts into the minor groove of salmon sperm DNA,and forms stable compound with DNA.

Key words: multi-quaternary ammonium salt, salmon sperm DNA, interaction, non-viral vector

CLC Number:

TQ423.12

LI Ya-ping,LAI Lu,ZHONG Zhou,MEI Ping,CHEN Wu,LIU Yi. Study on the interaction of multi-quaternary ammonium salt with salmon sperm DNA[J].China Surfactant Detergent & Cosmetics, 2016, 46(8): 427-432.

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Study on the interaction of multi-quaternary ammonium salt with salmon sperm DNA

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