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日用化学工业 ›› 2016, Vol. 46 ›› Issue (7): 414-418.doi: 10.13218/j.cnki.csdc.2016.07.011

• 开发与应用 • 上一篇    下一篇

藤茶多酚分离纯化及其对口腔变形链球菌抑制作用研究

蒋旭东1,杨卫豪2,周传检2,卢凯玲2,林翠梧3,4   

  1. 1.广西科技大学 医学院,广西 柳州 545005;
    2.柳州两面针股份有限公司,广西 柳州 545006;
    3.广西大学 化学化工学院,广西 南宁 530004;
    4.广西高校应用化学技术与资源开发重点实验室,广西 南宁 530004
  • 出版日期:2016-07-22 发布日期:2019-04-18
  • 通讯作者: 林翠梧,教授,博士,电话:(0771)3275878,E-mail:860777598@qq.com。
  • 作者简介:蒋旭东(1974-),男,广西全州人,副教授,博士,电话:13617807735,E-mail:xudong666@gmail.com。
  • 基金资助:
    柳州两面针股份有限公司博士后基金资助项目(2013)

Separation and purification of polyphenols from Ampelopsis grossedentata and its inhibition effect on oral mutating streptococci

JIANG Xu-dong1,YANG Wei-hao2,ZHOU Chuan-jian2,LU Kai-ling2,LIN Cui-wu3,4   

  1. 1.School of Medicine,Guangxi University of Science and Technology,Liuzhou,Guangxi 545005,China;
    2.Liuzhou LMZ Co.,Ltd.,Liuzhou,Guangxi 545006,China;
    3.School of Chemistry and Chemical Engineering, Guangxi University,Nanning,Guangxi 530004,China;
    4.Guangxi Colleges and Universities Key Laboratory of Applied Chemistry Technology and Resource Development,Nanning,Guangxi 530004,China
  • Online:2016-07-22 Published:2019-04-18

摘要: 采用Folin-Ciocaileu比色法测定藤茶多酚质量浓度,计算吸附率和洗脱率,比较5种不同性质大孔树脂对藤茶多酚静态吸附和解吸的影响,优选出HPD-100型树脂。进一步通过单因素试验得到藤茶多酚分离纯化的优选工艺条件为:上样液藤茶多酚粗提物质量浓度2.4 g/L、流速2.0 BV/h、体积6.5 BV;洗脱剂乙醇体积分数60%、流速3 BV/h、体积7.5 BV。在此条件下,经大孔树脂处理后藤茶多酚质量分数由29.9%提高到69.8%。抑菌试验表明,经大孔树脂处理后的藤茶提取物具有显著抑制口腔变形链球菌生长的作用,其最低抑菌浓度为3.13 g/L。

关键词: 口腔护理, 藤茶多酚, 纯化, 变形链球菌

Abstract: Via the determination of polyphenol mass concentration by Folin-Ciocaileu colorimetry,calculation of the adsorption and elution rate,as well as comparison of effect of five kinds of macroporous resin on static adsorption and desorption rates of polyphenols from Ampelopsis grossedentata,HPD-100 resin was selected to conduct the optimization of purification technology by single factor tests.Results showed that the optimum purification conditions are:extract of polyphenol mass concentration of feed sample,2.4 g/L of 6.5 BV,flow rate,2.0 BV/h;elution with 7.5 BV of 60% ethanol at a flow rate of 3 BV/h.The mass fraction of polyphenols can be enhanced from 29.9% to 69.8%.The results showed that the inhibition activity of polyphenols from Ampelopsis grossedentata on growth of oral mutating streptococci is very obvious and the MIC value achieves 3.13 g/L.

Key words: oral care, polyphenols from Ampelopsis grossedentata, purification, mutating streptococci

中图分类号: 

  • TQ658.4+9