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日用化学工业（中英文）

日用化学工业 ›› 2015, Vol. 45 ›› Issue (3): 152-156.doi: 10.13218/j.cnki.csdc.2015.03.007

• 开发与应用 • 上一篇    下一篇

指甲花醌的DNA毒性及机理研究

李蔚仑, 李军生, 黄国霞, 阎柳娟, 高颜   

  1. 广西科技大学 生物与化学工程学院 广西糖资源绿色加工重点实验室和广西高校糖资源加工重点实验室,广西 柳州 545006
  • 出版日期:2015-03-22 发布日期:2019-04-02
  • 通讯作者: 李军生,博士,教授,E-mail:junshenglee 63@aliyun.com。
  • 作者简介:李蔚仑(1988-),男,硕士研究生,E-mail:lwllwllwllwllwl@163.com。
       
  • 基金资助:
    广西自然科学基金资助项目(2010GXNSFA013134)

Study on DNA toxicity of lawsone and its mechanism

LI Wei-lun, LI Jun-sheng, HUANG Guo-xia, YAN Liu-juan, GAO Yan   

  1. Guangxi Key Laboratory of Green Processing of Sugar Resources and Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes,College of Biological and Chemical Engineering, Guangxi University of Science and Technology,Liuzhou,Guangxi 545006,China
  • Online:2015-03-22 Published:2019-04-02

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摘要： 采用双荧光探针法和共振散射光谱法研究了指甲花醌与DNA的作用,以结合饱和值评价指甲花醌的DNA毒性并推断其毒性机理。结果发现,指甲花醌与DNA的结合饱和值为0.33;在氯化钠、氯化钙、葡萄糖、异亮氨酸、组氨酸和维生素C这6种环境共存物对指甲花醌与DNA的结合影响最大时,指甲花醌与DNA的结合饱和值分别为0.40,0.50,0.29,0.33,0.25和0.29;指甲花醌可使溴化乙锭-DNA体系荧光强烈猝灭,使SYBR Green-DNA体系荧光微弱猝灭;且指甲花醌-双链DNA体系共振散射强度明显强于指甲花醌-单链DNA体系。结果表明,指甲花醌存在一定的DNA毒性,可与DNA发生嵌入结合和较弱的沟槽键合。

关键词: 指甲花醌, DNA毒性, 结合饱和值, 毒性机理

Abstract: Fluorescence spectrometry with two kinds of fluorescence probe and resonance light scattering spectroscopy were applied to study the interaction between lawsone and DNA.The DNA saturation binding value was adopted to evaluate the toxicity of the lawsone and its toxicity mechanism was inferred.Results showed that the DNA saturation binding value of lawsone was 0.33.In case the binding effect of six kinds of environmentally co-existing material,sodium chloride,calcium chloride,glucose,isoleucine,histidine and vitamin C achieving the highest value,the DNA saturation binding values of lawsone is 0.40,0.50,0.29,0.33,0.25 and 0.29 respectively.The lawsone can eliminate the fluorescence intensity of ethidium bromide-DNA abruptly and can eliminate that of SYBR Green-DNA slightly.Intensity of resonance light scattering of lawsone-single stranded DNA was weaker than that of lawsone-double stranded DNA.The conclusion is that the lawsone has certain toxicity.It can cause embedded binding with the DNA base pairs,and weakly bind into the minor groove of DNA.

Key words: lawsone, DNA toxicity, saturation binding value, toxicity mechanism

中图分类号: 

  • TQ658.3+4