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日用化学工业（中英文）

日用化学工业 ›› 2017, Vol. 47 ›› Issue (6): 357-360.doi: 10.13218/j.cnki.csdc.2017.06.012

• 分析与检测 • 上一篇    

高效液相色谱法同时测定洗发水中6种去屑剂

牛水蛟1, 唐子安2, 李启艳1, 王小兵1, 李俊婕1, 于海英1   

  1. 1.山东省食品药品检验研究院,山东 济南 250101;
    2.山东省食品药品监督管理局 审评认证中心,山东 济南 250014
  • 收稿日期:2017-01-19 发布日期:2019-03-20
  • 通讯作者: 于海英,硕士,电话:15953170391,E-mail:myhy12@163.com。
  • 作者简介:牛水蛟(1986-),男,山东人,硕士,电话:15066653029,E-mail:laoniule-001@163.com。

Simultaneous determination of six kinds of anti-dandruff agent in hair shampoo by high performance liquid chromatography

NIU Shui-jiao1, TANG Zi-an2, LI Qi-yan1, WANG Xiao-bing1, LI Jun-jie1, YU Hai-ying1   

  1. 1.Shandong Institute for Food and Drug Control,Jinan,Shandong 250101,China;
    2.Center for Drug Evaluation and Certification,Shandong Food and Drug Administration,Jinan,Shandong 250014,China
  • Received:2017-01-19 Published:2019-03-20

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摘要： 建立了同时测定洗发水中己脒定二(羟乙基磺酸)盐、水杨酸、吡硫翁锌、酮康唑、氯咪巴唑和吡罗克酮乙醇胺盐6种去屑剂的高效液相色谱法。采用Atlantis T3色谱柱(4.6 mm×250 mm,5 μm),以乙腈-甲醇-0.01 mol/L磷酸二氢钾水溶液(含乙二胺四乙酸二钠0.5 mmol/L)为流动相进行梯度洗脱,流速1.0 mL/min,柱温室温,进样量5 μL。结果表明,己脒定二(羟乙基磺酸)盐、水杨酸、酮康唑和氯咪巴唑的线性范围为4~200 mg/L,吡硫翁锌的线性范围为2~100 mg/L,吡罗克酮乙醇胺盐的线性范围为5~250 mg/L,方法的线性关系良好,相关系数均大于0.999。6种去屑剂的平均回收率为96.2%~102.0%,日间和日内精密度分别为0.17%~0.89%和0.27%~1.30%,方法检出限为5~25 mg/kg,定量限为15~75 mg/kg。

关键词: 洗发水, 去屑剂, 高效液相色谱法

Abstract: A method for simultaneous determination of six kinds of anti-dandruff agent including hexamidine diisethionate,salicylic acid,pyrithione zinc,ketoconazole,climbazole and octopirox in hair shampoo was established by high performance liquid chromatography with an Atlantis T3 column (4.6 mm×250 mm,5 μm).A mixture of acetonitrile-methanol aqueous solution (0.01 mol/L KH2PO4 and 0.5 mmol/L Na2EDTA) was at a flow rate of 1.0 mL/min used as HPLC mobile phase for gradient elution at room temperature;while 5 μL of which was injected for analysis.Following are the results.The linearity range of hexamidine diisethionate,salicylic acid,ketoconazole and climbazole is 4-200 mg/L,pyrithione zinc is 2-100 mg/L,as well as 5-250 mg/L for octopirox.All of which have a fine linear relation with the correlation coefficients greater than 0.999.The spiked recoveries of six components vary from 96.2% to 102.0%.The relative standard deviations of intra and inter day precision are 0.17%-0.89% and 0.27%-1.30%.The limits of detection range from 5 to 25 mg/kg,also 15-75 mg/kg as the limits of quantification.

Key words: shampoo, anti-dandruff agent, high performance liquid chromatography

中图分类号: 

  • TQ658.3+3