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日用化学工业（中英文）

日用化学工业 ›› 2014, Vol. 44 ›› Issue (2): 112-114.doi: 10.13218/j.cnki.csdc.2014.02.026

• 分析与检测 • 上一篇    下一篇

反相高效液相色谱法测定化妆品中的神经酰胺

缪晓琴, 金抒   

  1. 南京野生植物综合利用研究院,江苏 南京 210042
  • 收稿日期:2013-07-03 发布日期:2019-04-09
  • 通讯作者: 金 抒,电话:13022599490,E-mail:sjinlab@msn.com。
  • 作者简介:缪晓琴(1976-),女,电话:(025)85282343,E-mail:miaomiaoy@sina.com。

Determination of ceramides by reversed phase high performance liquid chromatography

MIAO Xiao-qin, JIN Shu   

  1. Nanjing Research Institute for Comprehensive Utilization of Wild Plants,Nanjing,Jiangsu 210042,China
  • Received:2013-07-03 Published:2019-04-09

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摘要： 采用反相高效液相色谱法测定化妆品中的神经酰胺。色谱条件为:SHIMADZU Shim-pack VP-C18ODS (150 mm×4.6 mm,5 μm)色谱柱,C18ODS保护柱芯,流动相V(甲醇)∶V(异丙醇)=55∶45,流速1.0 mL·min-1,柱温30 ℃,紫外检测器检测,波长205 nm,检测池温度40 ℃。结果表明,在此色谱条件下,神经酰胺在0～5 g·L-1与相应的峰面积具有良好的线性关系(r=0.991 4),线性回归方程为A=1 466 341ρ,回收率在96.5%～101.2%。

关键词: 化妆品添加剂, 神经酰胺, 高效液相色谱法

Abstract: A reversed phase high performance liquid chromatography (RP-HPLC) method for the determination of ceramides in cosmetics was established.The sample was analyzed on a SHIMADZU Shim-pack VP-C18ODS (150 mm×4.6 mm,5 μm) with C18ODS guard column core and UV detector.Operating conditions were:mobile phase consisted of V(methanol)∶V(isopanol)=55∶45 at a flow rate of 1.0 mL·min-1;column temperature 30 ℃;wavelength of the UV detector 205 nm;detection cell temperature 40 ℃.Results showed that as the mass concentration of ceramide is in the range of 0-5 g·L-1,the relationship between mass concentration and the corresponding peak area shows good linearity (r=0.991 4).The regression equation can be expressed as A=1 466 341ρ and the recovery achieves 96.5%-101.2%.

Key words: additive of cosmetics, ceramide, high performance liquid chromatography

中图分类号: 

  • TQ658