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日用化学工业（中英文）

日用化学工业（中英文） ›› 2026, Vol. 56 ›› Issue (3): 400-406.doi: 10.3969/j.issn.2097-2806.2026.03.016

• 分析与检测 • 上一篇    

高效液相色谱法同时测定口腔护理牙膏中5种甜味剂含量

周艳芳1,*(),霍卫红1,杨海新1,陈雪菁2   

  1. 1 濮阳医学高等专科学校， 河南 濮阳 457000
    2 濮阳市人民医院， 河南 濮阳 457000
  • 收稿日期:2025-04-14 修回日期:2026-03-03 出版日期:2026-03-22 发布日期:2026-05-14
  • 通讯作者: 周艳芳

Simultaneous determination of 5 kinds of sweeteners in oral care toothpaste by HPLC

Yanfang Zhou1,*(),Weihong Huo1,Haixin Yang1,Xuejing Chen2   

  1. 1 Puyang Medical College, Puyang, Henan 457000, China
    2 Puyang People’s Hospital, Puyang, Henan 457000, China
  • Received:2025-04-14 Revised:2026-03-03 Online:2026-03-22 Published:2026-05-14
  • Contact: Yanfang Zhou

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摘要：

建立了高效液相色谱法（HPLC）同时检测口腔护理牙膏中5种甜味剂（糖精钠、阿斯巴甜、安赛蜜、三氯蔗糖、甜菊糖苷）。该方法使用水-甲醇（体积比80∶20）混合溶液作为提取溶剂。超声参数为：时间20 min，温度40 ℃，超声功率360 W。超声后用固相萃取净化。采用配备DAD-3000 RS二极管阵列检测器的Thermo Scientific UltiMate 3000液相色谱系统进行检测。Capcell Pak MGⅡ C18色谱柱（250 mm×4.6 mm, 5 μm），柱温35 ℃。流动相为20 mmol/L乙酸铵溶液（pH 5.5）和甲醇。检测波长为214 nm。方法学评价结果表明，5种甜味剂的精密度RSD均优于5%；在0.1~500 mg/L质量浓度范围内5种甜味剂呈良好的线性关系，相关系数在0.999 4~0.999 8区间，检测限为0.05~0.20 mg/L；加标回收实验中5种甜味剂的加标平均回收率在97.08%~105.53%之间，批次内RSD值均优于5%。

关键词: 高效液相色谱法, 同时测定, 牙膏, 甜味剂, 口腔

Abstract:

In this study, a high performance liquid chromatography （HPLC） method was established for the simultaneous determination of 5 sweeteners （saccharin sodium, aspartame, acesulfame, sucralose, and stevia） in oral care toothpaste. The mixed solution of water-methanol （volume ratio of 80∶20） was used as the extraction solvent. Ultrasonic parameters were as follows: time 20 min, temperature 40 ℃, and ultrasonic power 360 W. After ultrasonic extraction, solid phase extraction was used. The test was performed using the Thermo Scientific UltiMate 3000 liquid chromatography system with the DAD-3000 RS diode array detector. The chromatography was performed on Capcell Pak MG-Ⅱ C18 column （250 mm×4.6 mm, 5 μm） at 35 ℃. The mobile phase consisted of 20 mmol/L ammonium acetate solution （pH 5.5） and methanol. The detection wavelength was 214 nm. The results of the methodology evaluation show that the precision RSD of the five sweeteners are all less than 5%. In the mass concentration range of 0.1-500 mg/L, the five sweeteners show good linear relationships, the correlation coefficients are distributed in the range of 0.999 4-0.999 8, and the detection limits are 0.05-0.20 mg/L. The recovery rates of the five sweeteners range from 97.08% to 105.53%, and RSD values in batches are all lower than 5%.

Key words: high performance liquid chromatography, simultaneous determination, toothpaste, sweetener, oral

中图分类号: 

  • TQ658