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日用化学工业（中英文）

日用化学工业 ›› 2015, Vol. 45 ›› Issue (8): 457-461.doi: 10.13218/j.cnki.csdc.2015.08.009

• 开发与应用 • 上一篇    下一篇

纤维素酶活力测定条件研究及其在洗涤剂中的应用

于跃, 张剑, 何德志   

  1. 山西大学 化学化工学院,山西 太原 030006
  • 收稿日期:2015-05-26 修回日期:2015-07-27 出版日期:2015-08-22 发布日期:2019-04-10
  • 通讯作者: 张 剑,教授,博士,电话:13834110276,E-mail:zhangjian0923@vip.sina.com。
  • 作者简介:于 跃(1984-),男,辽宁人,硕士研究生,电话:15735172119,E-mail:yyy816@126.com。
  • 基金资助:
    山西省自然科学基金资助项目(2013011040-4); 山西省留学人员科技活动择优资助项目

Studies on conditions for determination of cellulase activity and its adoption for determination in cellulase formulated detergent products

YU Yue, ZHANG Jian, HE De-zhi   

  1. College of Chemistry and Chemical Engineering,Shanxi University,Taiyuan,Shanxi 030006,China
  • Received:2015-05-26 Revised:2015-07-27 Online:2015-08-22 Published:2019-04-10

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摘要： 采用纤维素酶羧甲基纤维素(CMC)酶活的测定方法,研究了温度、pH、波长及吸光度等条件对纤维素酶活力测定的影响。结果表明,在温度为50 ℃,pH=6,吸收波长为530 nm及吸光度为0.2~0.4的条件下,测得的纤维素酶活力数值较为稳定。在上述优选条件下添加液体洗涤剂和洗衣粉背景,考察该方法能否用于液体洗涤剂和洗衣粉中纤维素酶活力的测定。研究结果显示,此方法适用于液体洗涤剂和洗衣粉中纤维素酶活力的测定,但需严格控制洗涤剂或洗衣粉溶液的温度和pH。

关键词: 洗涤剂, 纤维素酶, 活力分析

Abstract: Determination of enzymatic activity of cellulase formulated with carboxymethylcellulose (CMC) was conducted.Effect of determination conditions including enzymatic reaction temperature,pH value,wavelength and absorbance on the analysis results were studied.Results showed that under conditions for determination of cellulase activity as temperature 50 ℃,pH value 6,wavelength 530 nm and absorbance 0.2~0.4,stable analysis results can be obtained.Further studying on the cellulase formulated in laundry liquid detergent and laundry powder showed that this method is suitable for measuring cellulase activity in laundry liquid detergent and laundry powder.However,the operating temperature and pH value should be strictly controlled.

Key words: detergent, cellulase, activity assay

中图分类号: 

  • TQ649.6+6