百子莲脱水素蛋白对人皮肤细胞损伤的保护作用

doi:10.3969/j.issn.1001-1803.2020.02.002

Abstract

Abstract:

Normal human skin fibroblast （NHSF） cells and HaCaT cells were applied to establish evaluation models of dehydration, light-aging and oxidative stress. Based on the models, the protective effects of Y₂SK₂ and SK₃ type dehydrins from Agapanthus praecox （ApY₂SK₂ and ApSK₃） on human skin cells were studied. The results showed that, the safe concentration of dehydrins was 0.01-10 μmol/L. Light-aging and oxidative stress could cause oxidative damage and apoptosis of human skin cells. 10 μmol/L ApSK₃ could increase the activity of skin cells by 57%-91% after cryopreservation. ApSK₃ could significantly improve the activity of skin cells under dehydration. Both the two dehydrins provided anti-oxidative protection for skin cells in the models of light-aging and oxidative stress, in which the level of malondialdehyde was decreased by 10%-36% and the activity of SOD was significantly increased for HaCaT cells. The activity of casepase-3 and casepase-8 was decreased by ApSK₃according to the oxidative stress model, and the occurrence of programmed cell death was reduced. The research showed that, Y₂SK₂ and SK₃ type dehydrins from Agapanthus praecox could protect human skin cells against stress damage, the mechanism of which could possibly be related to the enhanced cell anti-oxidation and their participation in the process of cell apoptosis.

Key words: dehydrin, HaCaT cell, NHSF cell, oxidative stress, cell apoptosis

CLC Number:

TQ658

LV Ke,LI Jing,ZHANG Di. Protection of human skin cells against stress damage by use of Y₂SK₂ and SK₃ type dehydrins from Agapanthus praecox[J].China Surfactant Detergent & Cosmetics, 2020, 50(2): 76-85.

Figures/Tables 6

Tab. 1

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References 27

[1]	Trouba K J, Hamadeh H K, Amin R P , et al. Oxidative stress and its role in skin disease[J]. Antioxid Redox Signal, 2002,4(4) : 665-673.
[2]	Liu L, Xie H, Chen X , et al. Differential response of normal human epidermal keratinocytes and HaCaT cells to hydrogen peroxide-induced oxidative stress[J]. Clinical and Experimental Dermatology, 2012,37(7) : 772-780.
[3]	Zhang Jie . Effect of H₂O₂ induction on expression of β-catenin and SMP30 in normal human skin fibrolasts[J]. Journal of Nanchang University (Medical Sciences), 2018,58(3) : 24-27.
[4]	Yang Na, Li Ming, Hu Jiadong , et al. Protective effects on dermal fibroblasts under the UVA oxidative stress by lycopene[J]. Biotechnology Bulletin, 2013 ( 11) : 142-147.
[5]	Tan C P, Lu Y Y, Ji L N , et al. Metallomics insights into the programmed cell death induced by metal-based anticancer compounds[J]. Metallomics, 2014,6(5) : 978-995.
[6]	Chen Guanqun . Study on the mechanism of cathepsin ApCathB in cryopreservation-induced programmed cell death in Agapanthus praecox ssp. orientalis[D]. Shanghai: Shanghai Jiao Tong University, 2019.
[7]	Galau G A, Hughes D W, Dure L . Abscisic acid induction of cloned cotton late embryogenesis-abundant (Lea) mRNAs[J]. Plant Molecular Biology. 1986,7(3) : 155-170.
[8]	Soulages J L . Conformation of a group 2 late embryogenesis abundant protein from soybean. evidence of poly (L-proline)-type II structure[J]. Plant Physiology, 2003,131(3) : 963-975.
[9]	Saibi W, Feki K, Ben Mahmoud R , et al. Durum wheat dehydrin (DHN-5) confers salinity tolerance to transgenic Arabidopsis plants through the regulation of proline metabolism and ROS scavenging system[J]. Planta, 2015,242(5) : 1187-1194.
[10]	Yang Z, Sheng J, Lv K , et al. Y₂SK₂ and SK₃ type dehydrins from Agapanthus praecox can improve plant stress tolerance and act as multifunctional protectants[J]. Plant Science, 2019,284:143-160.
[11]	Chen Luping, Wang Pu, Tang Hailiang , et al. Comparison of the effects of different cryoprotectants on cryopreservation and resuscitation of human induced pluripotent stem cells[J]. Chinese Journal Clinical Neurosciences, 2012,20(5) : 494-499.
[12]	Li Jing . Study on application of human skin cells protection by three dehydrins[D]. Shanghai: Shanghai Jiao Tong University, 2019.
[13]	Wei Li, Shao Yuling . Superoxide dismutase and dermatosis[J]. China J Lepr Dis, 2007,23(9) : 802-804.
[14]	Wu Haitang , Hung lianglian, Liang Bingzhong. The effects of antioxidants on pyridinoline cross linkage formation in human fibroblasts culture from hypertrophic scars[J]. China Journal of Modern Medicine, 2002 ( 9) : 590-592.
[15]	Zhang Yongxiang, Guo Shilei, Peng Lisha . Protective effect of Aronia melanocarpa anthocaynins against UV-induced damages of human skin fibroblast[J]. Nature Product Research Development, 2016 ( 7) : 1088-1092.
[16]	Zhang Huan . Study on Eucommia active ingredient screening of anti-aging skin and its mechanism[D]. Zhengzhou: Henan University, 2016.
[17]	Rosales R, Romero I, Escribano M I , et al. The crucial role of Φ- and K-segments in the in vitro functionality of Vitis vinifera dehydrin DHN1a[J]. Phytochemistry, 2014,10(8) : 17-25.
[18]	Zhang D, Ren L, Chen G Q , et al. ROS-induced oxidative stress and apoptosis-like event directly affect the cell viability of cryopreserved embryogenic callus in Agapanthus praecox [J]. Plant Cell Reports, 2015,34(9) : 1499-1513.
[19]	Tan C P, Lu Y Y, Ji L N , et al. Metallomics insights into the programmed cell death induced by metal-based anticancer compounds[J]. Metallomics, 2014,6(5) : 978.
[20]	Lee Y J, Kang I J, Rolf Bünger , et al. Mechanisms of pyruvate inhibition of oxidant-induced apoptosis in human endothelial cells[J]. Microvascular Research, 2003,66(2) : 0-101.
[21]	Susztak K . Glucose-induced reactive oxygen species cause apoptosis of podocytes and podocyte depletion at the onset of diabetic nephropathy[J]. Diabetes, 2006,55(1) : 225-233.
[22]	Menini S, Amadio L, Oddi G , et al. Deletion of p66Shc longevity gene protects against experimental diabetic glomerulopathy by preventing diabetes-induced oxidative stress[J]. Diabetes, 2006,55(6) : 1642-1650.
[23]	Chen M, Wang J . Initiator caspases in apoptosis signaling pathways[J]. Apoptosis, 2002,7(4) : 313-319.
[24]	Mcdonald E R, El-Deiry W S . Suppression of caspase-8-and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth[J]. Proceedings of the National Academy of Sciences, 2004,101(16) : 6170-6175.
[25]	Porter A G, Nicke R U . Emerging roles of casepase-3 in apoptosis[J]. Cell Death and Differentiation, 1999,6(2) : 99-104.
[26]	Liu S H, Ma K, Xu X B . A single dose of carbon monoxide intraperitoneal administration protects rat intestine from injury induced by lipopolysaccharide[J]. Cell Stress & Chaperones, 2010,15(5) : 717-727.
[27]	Cong Jing, Wu Jingdong . Research progresses of fibroblast in skin aging[J]. Chinese Journal of Aesthetic, 2009,18(5) : 736-738.

组别	蛋白浓度/（μmol·L^-1）	HaCaT细胞增殖率/%			NHSF细胞增殖率/%
不添加脱水素空白组	0	100			100
添加ApY₂SK₂实验组	0.01	96.65	±	9.56	96.07	±	6.78
	0.05	103.17	±	10.64	95.24	±	11.22
	0.1	114.79	±	11.33	111.87	±	7.50
	0.5	124.47	±	12.64^*	111.49	±	7.82
	1	127.99	±	9.54^*	118.14	±	10.97^*
	5	133.45	±	11.11^*	128.72	±	10.24^*
	10	135.56	±	16.90^*	116.70	±	3.96^*
添加ApSK₃实验组	0.01	97.90	±	9.96	97.12	±	8.75
	0.05	110.23	±	6.17^*	102.34	±	16.90^*
	0.1	111.84	±	10.07^*	117.31	±	14.17^*
	0.5	124.78	±	8.52^*	118.33	±	14.96^*
	1	136.78	±	7.37^*	120.74	±	10.25^*
	5	126.11	±	8.94^*	132.24	±	15.23^*
	10	120.96	±	9.85^*	127.85	±	22.26

Protection of human skin cells against stress damage by use of Y₂SK₂ and SK₃ type dehydrins from Agapanthus praecox

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Protection of human skin cells against stress damage by use of Y₂SK₂ and SK₃ type dehydrins from Agapanthus praecox