复方紫草凝胶外用制剂的抗炎与抑菌作用研究

doi:10.3969/j.issn.1001-1803.2020.12.006

Abstract

Abstract:

The optimal formulation of compound radix arnebiae gel topical preparation （CRAGTP） was screened by ternary phase diagram. Three acute inflammation models （xylene-induced ear swelling, xylene-induced abdominal skin capillary permeability increase, and acetic acid-induced abdominal cavity capillary permeability increase） were used to study the anti-inflammatory effects of CRAGTP. Disc diffusion test （K-B method） was used to study the inhibitory effect of CRAGTP on Staphylococcus aureus （SA）, Escherichia coli （E.coli） and Pseudomonas areuginas （PA） in vitro. Preliminary results show that the optimal composition of CRAGTP formulation is 0.5% of compound radix arnebiae extract, 26.0% of oil phase mixture, 40.2% of surfactants, 3.3% of glycerin and 30.0% of water. The formulated gel is clear and transparent, with pH of 6.49 and particle size of 22.64 nm. CRAGTP shows great ability to inhibit the xylene-induced ear swelling （P<0.01）, xylene-induced increasing of abdominal skin capillary permeability （P<0.01）, and acetic acid-induced increasing of abdominal cavity capillary permeability （P<0.05）. It has an inhibitory effect on SA and E.coli, but has little inhibitory effect on PA. CRAGTP has a good anti-inflammatory effect and a certain antibacterial activity.

Key words: compound radix arnebiae gel topical preparation, surfactant, inflammation, antibacterial activity, xylene

CLC Number:

TQ423

JIA Ying,WANG Jia-min,WANG Shi-hui,LIU Kai-li,YANG Da-wei,WANG Yan. Study on the anti-inflammatory and antibacterial effects of compound radix arnebiae gel topical preparation[J].China Surfactant Detergent & Cosmetics, 2020, 50(12): 848-853.

Figures/Tables 9

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References 16

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类别	辅料
油相	橄榄油、霍霍巴油
表面活性剂	聚氧乙烯40氢化蓖麻油（RH 40）、司班80（Span 80）
助表面活性剂	无水乙醇、甘油、PEG-400

无抑菌作用	有抑菌作用	敏感程度
	6≤d<10	低度敏感
d<6	10≤d<15	中度敏感
	15≤d	高度敏感

类别	辅料	w/%
提取物	复方紫草提取物	0.5
油相	m（橄榄油）:m（霍霍巴油）=1:4	26.0
表面活性剂	m（RH 40）:m（Span 80）=6:4	40.2
助表面活性剂	甘油	3.3
水相	水	30.0

组别	剂量/mg	耳肿胀度/mg	耳肿胀抑制率/%
空白组	40	0.25±0.01^**	—
模型组	40	3.29±0.81^△△	—
阳性药组	40	1.49±0.76^**	54.71
空白凝胶组	40	1.90±0.69^**	42.25
CRAGTP组	40	0.83±0.45^**#	74.77

组别	剂量/（mg·cm^-2）	吸光度值	组别	剂量/（mg·cm^-2）	吸光度值
空白组	45	0.042±0.008^**	空白凝胶组	45	0.065±0.006^**
模型组	45	0.224±0.079^△△	CRAGTP组	45	0.052±0.018^**
阳性药组	45	0.051±0.017^**

Study on the anti-inflammatory and antibacterial effects of compound radix arnebiae gel topical preparation

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组别	剂量/（mg·cm^-2）	吸光度值
空白组	45	0.290±0.031^*
模型组	45	0.496±0.099^△
阳性药组	45	0.352±0.071
空白凝胶组	45	0.385±0.089
CRAGTP组	45	0.212±0.109^*

药物	抑菌环直径/mm
药物	SA	E.coli
盐酸米诺环素胶囊	24.67±2.08	18.07±1.01
0.50% CRAGTP	16.33±1.53	13.13±2.20
0.25% CRAGTP	12.33±2.08	9.60±1.97
0.13% CRAGTP	9.17±1.76	0
0.06% CRAGTP	0	0
空白凝胶	0	0

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