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日用化学工业（中英文）

日用化学工业 ›› 2016, Vol. 46 ›› Issue (9): 544-548.doi: 10.13218/j.cnki.csdc.2016.09.011

• 分析与检测 • 上一篇    

双波长HPLC法同时测定植物防晒复配物中7种防晒成分的含量

岳爱国1,刘纲勇2,王如意2,刘经亮2   

  1. 1.无限极中国有限公司,广东 广州 510623;
    2.广东食品药品职业学院,广东 广州 510520
  • 出版日期:2016-09-22 发布日期:2019-04-18
  • 通讯作者: 刘纲勇,副教授,博士,电话:(020)28854912,E-mail:liugy@gdyzy.edu.cn。
  • 作者简介:岳爱国(1969-),男,湖南人,工程师,学士,电话:(020)80734703,E-mail:Patras.yue@infinitus-int.com。

Simultaneous determination of seven kinds of sunscreen component in blend of plant extract by RP-HPLC

YUE Ai-guo1,LIU Gang-yong2,WANG Ru-yi2,LIU Jing-liang2   

  1. 1.Infinitus China Co.Ltd.,Guangzhou,Guangdong 510623,China;
    2.Guangdong Food and Drug Vocational College,Guangzhou,Guangdong 510520,China
  • Online:2016-09-22 Published:2019-04-18

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摘要： 建立了双波长高效液相色谱法(RP-HPLC)同时测定自制植物防晒复配物中绿原酸、芦丁、黄芩苷、丹皮酚、黄芩素、大黄素和大黄酚7种防晒成分的含量。样品用纯甲醇溶解,采用WondaSil C18 WR色谱柱(250 mm×4.6 mm,5 μm)分离,以甲醇和0.1%磷酸溶液为流动相进行梯度洗脱,流速1.0 mL/min,检测波长270和327 nm,柱温40 ℃。结果表明,绿原酸、芦丁、黄芩苷、丹皮酚、黄芩素、大黄素和大黄酚的线性范围分别为4.24~42.4,6.76~67.6,4.16~41.6,4.90~49.0,4.34~43.4,4.28~42.8和5.72~57.2 ng,线性相关系数均大于0.999。7种防晒成分的平均回收率为 92.4%~107.8%,RSD为3.81%~7.38%。该方法操作简便、准确、重复性好,可用于自制植物防晒复配物的质量控制。

关键词: 化妆品添加剂, 防晒, 复配, 高效液相色谱法

Abstract: A RP-HPLC method was established for simultaneous determining chlorogenic acid,lutin,baicalin,paeonol,baicalein,emodin and chrysophanol in blend of plant extract.The sample was extracted by high purity methanol.The separation was performed on a WondaSil C18 WR column (250 mm×4.6 mm,5 μm);with methanol and 0.1% H3PO4 solution as mobile phase.The flow rate was 1.0 mL/min,the detection wavelength was 270 and 327 nm,and the column temperature was 40 ℃.Results showed that,the linearity ranges within 4.24-42.4 ng for chlorogenic acid,6.76-67.6 ng for lutin,4.16-41.6 ng for baicalin,4.90-49.0 ng for paeonol,4.34-43.4 ng for baicalein,4.28-42.8 ng for emodin,and 5.72-57.2 ng for chrysophanol and all correlation coefficients are higher than 0.999.The average spiked recovery rates are from 92.4% to 107.8%,and the relative standard deviations (RSD) are from 3.81% to 7.38%.The method is simple,accurate and suitable for the quality control for the preparation of such blend plant extracts.

Key words: cosmetic additives, sunscreen, blend, HPLC

中图分类号: 

  • TQ658