高效液相色谱法测定牙膏中叶绿素铜钠盐的含量

doi:10.13218/j.cnki.csdc.2018.11.011

日用化学工业 ›› 2018, Vol. 48 ›› Issue (11): 661-664.doi: 10.13218/j.cnki.csdc.2018.11.011

• 分析与检测 • 上一篇

高效液相色谱法测定牙膏中叶绿素铜钠盐的含量

李玉静, 王雪平

黄河水利职业技术学院,河南开封 475004

收稿日期:2018-06-05 出版日期:2018-11-22 发布日期:2019-03-18
作者简介:李玉静(1978-),女,河南南阳人,讲师,硕士,研究方向：化学工程,电话：18236788599,Email：979922905@qq.com。
基金资助:
河南省科技厅支持项目(172102410035); 黄河水利职业技术学院2016年科技项目(2016KXJS009)

Determination of sodium copper chlorophyllin in toothpaste by high performance liquidchromatography

LI Yu-jing, WANG Xue-ping

Yellow River Conservancy Technical Institute,Kaifeng,Henan 475004,China

Received:2018-06-05 Online:2018-11-22 Published:2019-03-18

摘要/Abstract

摘要： 在酸性条件下,以聚酰胺粉吸附牙膏中叶绿素铜钠盐,解吸附洗脱,经YMC Carotenoid C₃₀ (4.6 mm×250 mm,3 μm)色谱柱分离,光二极管阵列检测器(PDA)检测,建立了高效液相色谱法测定牙膏中叶绿素铜钠盐含量的方法。样品经甲醇-叔丁基甲醚-0.5%醋酸混合溶液为流动相进行梯度洗脱,流速1.0 mL/min,柱温30 ℃,检测波长405 nm。结果表明,叶绿素a铜钠盐和叶绿素b铜钠盐在5.0~100.0 mg/L质量浓度范围内,具有较好的线性关系,相关系数分别为0.999 8和0.999 7;检出限均为1.5 μg/kg,定量限均为5.0 μg/kg;加标回收率为87.2%~97.8%,RSD为1.7%~2.9%。

关键词: 牙膏, 叶绿素铜钠盐, 聚酰胺粉, 高效液相色谱法

Abstract: The sodium copper chlorophyllin in toothpaste was adsorbed by polyamide under acidic conditions,eluted by desorption,separated by YMC Carotenoid C₃₀ chromatographic column (4.6 mm×250 mm,3 μm),and detected by photodiode array detector (PDA).A method for the determination of sodium copper chlorophyllin in toothpaste by high performance liquid chromatography (HPLC) was established.The sample was subjected to gradient elution with methanol-tert-butyl methyl ether-0.5% acetic acid mixed solution as a mobile phase at a flow rate of 1.0 mL/min,a column temperature of 30 °C,and a detection wavelength of 405 nm.The results show that sodium copper chlorophyllin a and sodium copper chlorophyllin b have a good linear relationship in the range of 5.0-100.0 mg/L,and the correlation coefficients are 0.999 8 and 0.999 7 respectively.The detection limits are 1.5 μg/kg;the limits of quantitation are 5.0 μg/kg;the recovery rate is 87.2%-97.8% and the relative standard deviation is 1.7%-2.9%.

Key words: toothpaste, sodium copper chlorophyllin, polyamide, high performance liquid chromatography

中图分类号:

TQ658.4

李玉静, 王雪平. 高效液相色谱法测定牙膏中叶绿素铜钠盐的含量[J]. 日用化学工业, 2018, 48(11): 661-664.

LI Yu-jing, WANG Xue-ping. Determination of sodium copper chlorophyllin in toothpaste by high performance liquidchromatography[J]. China Surfactant Detergent & Cosmetics, 2018, 48(11): 661-664.

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高效液相色谱法测定牙膏中叶绿素铜钠盐的含量

Determination of sodium copper chlorophyllin in toothpaste by high performance liquidchromatography

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