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日用化学工业（中英文）

日用化学工业（中英文） ›› 2025, Vol. 55 ›› Issue (1): 41-48.doi: 10.3969/j.issn.2097-2806.2025.01.005

• 开发与应用 • 上一篇    下一篇

解毒消癥饮改良方的缓解皮肤炎症及机制研究

章玲1,2,刘静1,2,赵炳天1,2,*(),杨成1,2   

  1. 1.江南大学 化学与材料工程学院，江苏 无锡 214122
    2.江南大学 合成与生物胶体教育部重点实验室，江苏 无锡 214122
  • 收稿日期:2024-03-01 修回日期:2025-01-02 出版日期:2025-01-22 发布日期:2025-01-23

Study on relieving skin inflammation and mechanisms of Modified Jiedu Xiaozheng Yin

Ling Zhang1,2,Jing Liu1,2,Bingtian Zhao1,2,*(),Cheng Yang1,2   

  1. 1. School of Chemical and Materials Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China
    2. Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, China
  • Received:2024-03-01 Revised:2025-01-02 Online:2025-01-22 Published:2025-01-23
  • Contact: E-mail: btzhao@jiangnan.edu.cn.

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摘要：

文章旨在寻求解毒消癥饮改良方（MJXY）的协同抗炎最佳配比，并探究其对脂多糖（LPS）诱导的RAW264.7巨噬细胞的体外抗炎活性及相关机制。以一氧化氮（NO）和2, 2’-联氮-双（3-乙基-苯并噻唑-6-磺酸）二铵盐（ABTS）自由基清除率为指标，对MJXY中不同提取组分的抗炎和抗氧化活性进行评价。依据协同效应得分情况，分别得到最佳ABTS清除（m（白花蛇舌草）:m（鹿衔草）:m（夏枯草）:m（苦参）=20:5:6:6）和NO清除改良方（m（白花蛇舌草）:m（鹿衔草）:m（夏枯草）:m（苦参）=12:12:4:3）。通过体外细胞实验，发现MJXY可以显著降低细胞内的活性氧水平，同时能够抑制细胞炎症因子的分泌。进一步的蛋白印迹实验结果表明MJXY可以通过下调炎症信号通路中JAK2和STAT3蛋白磷酸化水平发挥抗炎作用。综上所述，MJXY具有较好的抗炎效果，并且可以通过干预JAK2/STAT3信号通路起到改善皮肤炎症的效果。

关键词: 解毒消癥饮, RAW264.7细胞, 皮肤炎症, JAK2/STAT3信号通路

Abstract:

The aim of this study was to find the best synergistic anti-inflammatory ratio of Modified Jiedu Xiaozheng Yin （MJXY）, and to explore its anti-inflammatory effect and related mechanisms through lipopolysaccharide （LPS）-induced RAW264.7 macrophages in vitro. The anti-inflammatory and antioxidant activities of different extracted components in MJXY were evaluated by the scavenging rates of nitric oxide （NO） and 2, 2’-azinobis-（3-ethylbenzthiazoline-6-sulphonate） （ABTS）. According to the synergistic effect scores, the best ABTS （m（Scleromitrion diffusum）:m（Pyrola calliantha）:m（Prunella vulgaris）:m（Sophora flavescens）=20:5:6:6） and NO scavenging formulation （m（Scleromitrion diffusum）:m（Pyrola calliantha）:m（Prunella vulgaris）:m（Sophora flavescens）=12:12:4:3） were obtained. It is found that MJXY can significantly reduce the level of reactive oxygen species in cells, and inhibit the secretion of inflammatory cytokines. Furthermore, western blot experiments show that MJXY can exert anti-inflammatory effects by down-regulating the phosphorylation levels of JAK2 and STAT3 in the inflammatory signaling pathway. In summary, MJXY has a good anti-inflammatory effect and can improve skin inflammation by inhibiting the JAK2/STAT3 signaling pathway.

Key words: Jiedu Xiaozheng Yin, RAW264.7 cell, skin inflammation, JAK2/STAT3 signaling pathway

中图分类号: 

  • TQ658