表没食子儿茶素没食子酸酯脂质体的制备及其透皮性能研究

doi:10.3969/j.issn.1001-1803.2020.09.005

Abstract

Abstract:

Epigallocatechin gallate （EGCG） liposomes （EL） were prepared by membrane-ultrasonic dispersion using soybean lecithin and cholesterol as encapsulation materials. The effects of the amount of cholesterol, hydration temperature, ultrasonic time, EGCG addition amount on the particle size, PDI, entrapment efficiency and yield of EL were studied. Also, the transdermal performance and the storage stability of EL were measured. The results show that, when the mass ratio of lecithin to cholesterol is 1∶0.25, the ultrasonic time is 6 min, the hydration temperature is 50 ℃, and the mass ratio of EGCG to lecithin is 0.075∶1, the high encapsulation efficiency and yield are achieved as 84.75%±2.12% and 93.78%±2.16%, and the particle size is （132.74±1.03） nm, the PDI is 0.245±0.009. According to the storage stability and transdermal experiments, liposomes can improve the transdermal transport and stability of EGCG significantly compared with the EGCG aqueous solution.

Key words: epigallocatechin gallate liposomes, preparation, skin penetration, stability

CLC Number:

TQ658

CAO Zhen-da,LIANG Rong. Study on the preparation and transdermal performance of epigallocatechin gallate liposome[J].China Surfactant Detergent & Cosmetics, 2020, 50(9): 609-614.

Figures/Tables 6

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

References 21

[1]	Guo Q, Zhao B L, Shen S R , et al. ESR study on the structure-antioxidant activity relationship of tea catechins and their epimers[J]. Biochimica Et Biophysica Acta-General Subjects, 1999,1427(1) : 13-23. doi: 10.1016/S0304-4165(98)00168-8
[2]	Krupkova O, Ferguson S J, Wuertz-Kozak K . Stability of (-)- epigallocatechin gallate and its activity in liquid formulations and delivery systems[J]. The Journal of Nutritional Biochemistry, 2016,37:1-12. doi: 10.1016/j.jnutbio.2016.01.002 pmid: 27770867
[3]	Wisuitiprot W, Somsiri A, Ingkaninan K , et al. In vitro human skin permeation and cutaneous metabolism of catechins from green tea extract and green tea extract-loaded chitosan microparticles[J]. International Journal of Cosmetic Science, 2011,33(6) : 572-579. doi: 10.1111/j.1468-2494.2011.00673.x
[4]	Marwah M, Perrie Y, Badhan R K S, et al. Intracellular uptake of EGCG-loaded deformable controlled release liposomes for skin cancer[J]. Journal of Liposome Research, 2019: 1-14. doi: 10.1080/08982104.2020.1748646 pmid: 32223352
[5]	Jin B S, Gwak H J . Preparation and characterization of EGCG entrapped ethosome[J]. Applied Chemistry for Engineering, 2007,18(2) : 130-135.
[6]	Xue H, Li X, Kuang C , et al. Research progress in antioxidant mechanisms of curcumin and development of antioxidant curcuminoids[J]. Food Science, 2010,31(7) : 302-307. doi: 10.7506/spkx1002-6300-201007067
[7]	Trotta M, Peira E, Carlotti M E , et al. Deformable liposomes for dermal administration of methotrexate[J]. International Journal of Pharmaceutics, 2004,270(1/2) : 119-125. doi: 10.1016/j.ijpharm.2003.10.006
[8]	Luo X, Guan R, Chen X , et al. Optimization on condition of epigallocatechin-3-gallate (EGCG) nanoliposomes by response surface methodology and cellular uptake studies in Caco-2 cells[J]. Nanoscale Research Letters, 2014,9(1) : 291. doi: 10.1186/1556-276X-9-291 pmid: 24959109
[9]	Wissing S A, Müller R H . The influence of the crystallinity of lipid nanoparticles on their occlusive properties[J]. International Journal of Pharmaceutics, 2002,242(1/2) : 377-379.
[10]	Chen W, Zou M, Ma X , et al. Co‐encapsulation of EGCG and quercetin in liposomes for optimum antioxidant activity[J]. Journal of Food Science, 2019,84(1) : 111-120. doi: 10.1111/1750-3841.14405 pmid: 30548488
[11]	Moghassemi S, Hadjizadeh A . Nano-niosomes as nanoscale drug delivery systems: an illustrated review[J]. Journal of Controlled Release, 2014,185:22-36. doi: 10.1016/j.jconrel.2014.04.015
[12]	Lu Q, Li D C, Jiang J G . Preparation of a tea polyphenol nanoliposome system and its physicochemical properties[J]. Journal of Agricultural and Food Chemistry, 2011,59(24) : 13004-13011. doi: 10.1021/jf203194w
[13]	Mandal S, Banerjee C, Ghosh S , et al. Modulation of the photophysical properties of curcumin in nonionic surfactant (Tween-20) forming micelles and niosomes: a comparative study of different microenvironments[J]. The Journal of Physical Chemistry B, 2013,117(23) : 6957-6968. doi: 10.1021/jp403724g pmid: 23682632
[14]	Li D, Martini N, Wu Z , et al. Development of an isocratic HPLC method for catechin quantification and its application to formulation studies[J]. Fitoterapia, 2012,83(7) : 1267-1274. doi: 10.1016/j.fitote.2012.06.006
[15]	Liang R, Chen L, Yokoyama W , et al. Niosomes consisting of tween-60 and cholesterol improve the chemical stability and antioxidant activity of (-)-epigallocatechin gallate under intestinal tract conditions[J]. Journal of Agricultural and Food Chemistry, 2016,64(48) : 9180-9188. doi: 10.1021/acs.jafc.6b04147 pmid: 27933988
[16]	Rashidinejad A, Birch E J, Sun-Waterhouse D , et al. Delivery of green tea catechin and epigallocatechin gallate in liposomes incorporated into low-fat hard cheese[J]. Food Chemistry, 2014,156:176-183. doi: 10.1016/j.foodchem.2014.01.115
[17]	Simon G A, Maibach H I . The pig as an experimental animal model of percutaneous permeation in man: qualitative and quantitative observations: an overview[J]. Skin Pharmacology and Physiology, 2000,13(5) : 229-234. doi: 10.1159/000029928
[18]	Klang V, Schwarz J C, Lenobel B , et al. In vitro vs. in vivo tape stripping: validation of the porcine ear model and penetration assessment of novel sucrose stearate emulsions[J]. European Journal of Pharmaceutics and Biopharmaceutics, 2012,80(3) : 604-614. doi: 10.1016/j.ejpb.2011.11.009
[19]	Marianecci C, Di Marzio L, Rinaldi F , et al. Niosomes from 80 s to present: the state of the art[J]. Advances in Colloid and Interface Science, 2014,205:187-206. doi: 10.1016/j.cis.2013.11.018
[20]	Sala M, Diab R, Elaissari A , et al. Lipid nanocarriers as skin drug delivery systems: Properties, mechanisms of skin interactions and medical applications[J]. International Journal of Pharmaceutics, 2018,535(1/2) : 1-17.
[21]	Verma D D, Verma S, Blume G , et al. Liposomes increase skin penetration of entrapped and non-entrapped hydrophilic substances into human skin: a skin penetration and confocal laser scanning microscopy study[J]. European Journal of Pharmaceutics and Biopharmaceutics, 2003,55(3) : 271-277. doi: 10.1016/s0939-6411(03)00021-3 pmid: 12754000

Study on the preparation and transdermal performance of epigallocatechin gallate liposome

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 21

Related Articles 15

Metrics

Comments

Recommended 10

[1]	Xiaohong Pan, Ziqi Gao, Zhen Chen, Shuai Yin, Haiping Huang, Bin Hu. Discussion on the current situation of research and management on the stability of cosmetic products in China [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 201-208.
[2]	Yaru Wang, Tingyuan Mo, Hongxia Lai, Yue Zhou, Jiaying Xie, Jianhua Tan. Analysis of the causes of skin irritation of niacinamide cosmetics based on patch test and stability test [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 51-56.
[3]	Zhen Enlong, Zhang Wen, Qian Zhen, Du Ruotong, Wang Yang. Construction and plugging performance evaluation of emulsified thermosetting resin system [J]. China Surfactant Detergent & Cosmetics, 2023, 53(6): 649-657.
[4]	Wang Huazheng, Zhang Liang, Kang Xin, Kang Wanli, Li Zhe, Yang Hongbin. Effect of CO₂ on physical properties of produced oil and water in Changqing and emulsion stabilization mechanism [J]. China Surfactant Detergent & Cosmetics, 2023, 53(6): 617-624.
[5]	Kong Lingniao, Hong Zheng, Fang Yinjun. Preparation of monoglyceride by glycerolysis of glyceryl tristearate catalyzed by Ce modified MgO [J]. China Surfactant Detergent & Cosmetics, 2023, 53(5): 517-522.
[6]	Zhan Xin, Xu Fan, Zhu Jun, He Yifan, Pei Xiaojing. Research progress on physiological action and preparations of luteolin [J]. China Surfactant Detergent & Cosmetics, 2023, 53(4): 437-444.
[7]	Ding Zhengqing, Wu Yingyi, Wang Weiyun, Huang Xujuan, Cai Zhaosheng. Study on Pickering emulsion stabilized by hydroxyethyl cellulose/nanocellulose and its rheological properties [J]. China Surfactant Detergent & Cosmetics, 2023, 53(3): 245-252.
[8]	Deng Mengjie, Yi Guobin, Lv Ran, Liu Yafeng, Ye Dawei, Chen Jiazhi. Ferulic acid nanoethosomes: preparation, characterization, and performance of skin penetration in vitro [J]. China Surfactant Detergent & Cosmetics, 2023, 53(11): 1285-1292.
[9]	Yang Chao, Tong Zhiming, Wang Zhansheng, Chen Wu. Study on the influence mechanisms of polymers and solid particles on the stability of crude oil emulsion [J]. China Surfactant Detergent & Cosmetics, 2023, 53(10): 1156-1165.
[10]	Guo Fang. Influence of emulsifiers and thickener on formation of low-viscosity liquid crystal emulsions [J]. China Surfactant Detergent & Cosmetics, 2023, 53(1): 16-23.
[11]	Yan Shaowei, Gao Chang, Zuo Lina. Preparation of high concentration ginsenoside Rg3 and its application in cosmetics [J]. China Surfactant Detergent & Cosmetics, 2023, 53(1): 24-31.
[12]	Yan Yongli,Cai Yuxiu,Dou Longlong,Cao Yuxia. Research progress in the dynamics of liquid drainage from complex foam system [J]. China Surfactant Detergent & Cosmetics, 2022, 52(9): 1011-1015.
[13]	Yu Xinqi,Sui Zhenquan,Xu Guiyun,Fan Jinshi. Common cosmetic preparation technologies （Ⅲ）Liquid, solid floating: Aerosol and spray [J]. China Surfactant Detergent & Cosmetics, 2022, 52(9): 930-936.
[14]	Hao Yang,Wang Changyun,Fan Jianru,Feng Yun,Li Zhenqiu,Xu Guiyun,Fan Jinshi. Common cosmetic preparation technologies （Ⅱ）Powerful controlled release tool: Microencapsulation [J]. China Surfactant Detergent & Cosmetics, 2022, 52(8): 805-811.
[15]	Feng Yun,Shi Jing,Bao Jie,Hao Yang,Xu Guiyun,Fan Jinshi. Common cosmetic preparation technologies（Ⅰ）Oil-water mixture: Emulsions [J]. China Surfactant Detergent & Cosmetics, 2022, 52(7): 696-703.