微波提取白茯苓美白成分的工艺研究

doi:10.3969/j.issn.1001-1803.2019.04.007

Abstract

Abstract:

The microwave-assisted extraction of skin-whitening agent from white Poria cocos was optimized by response surface methodology. Based on single factor experiment, the extraction solvent, liquid to solid ratio, extraction time, and microwave power were selected as independent variables and the relative inhibition rate of the extractive against tyrosinase was used as the response value. The Box-Behnken design and response surface method were used to optimize the extraction technology. The optimized conditions of extraction are as follows: distilled water is used as extraction solvent, with liquid to solid ratio of 10∶1（mL∶g）, extraction time of 7 min, microwave power of 520 W, and being extracted twice in one process. Under the optimized conditions, the yield of the extractive is 12.70%, and the relative inhibition rate is 31.10%, which is close to the theoretically predicted value（31.33%）.

Key words: skin-whitening agent in cosmetics, white Poria cocos, microwave-assisted extraction, tyrosinase, relative inhibition rate, response surface method

CLC Number:

TQ658

LI Zhi-ying,WANG Xue-han,GAO Rui-yuan. Microwave-assisted extraction technology of skin-whitening agent in white Poria cocos[J].China Surfactant Detergent & Cosmetics, 2019, 49(4): 238-243.

Figures/Tables 9

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References 16

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反应液	V/mL
反应液	1组	2组	3组	4组
1 g/L提取物溶液	0	0	2	2
pH=6.8缓冲溶液	4	5	2	3
0.1%酪氨酸溶液	2	2	2	2
0.3 g/mL酪氨酸酶溶液	1	0	1	0
反应液总体积	7	7	7	7

水平	液料比（A）/（mL·g^-1）	提取时间（B）/ min	微波功率（C）/ W
-1	9:1	5	325
0	10:1	7	455
1	11:1	9	585

方差来源	平方和	自由度	均方	F值	P值	显著性
模型	0.011	9	1.268×10^-3	43.64	0.000 3	***
A	4.651×10^-4	1	4.651×10^-4	16.01	0.010 3	*
B	2.645×10^-4	1	2.645×10^-4	9.10	0.029 5	*
C	2.531×10^-4	1	2.531×10^-4	8.71	0.031 8	*
AB	5.523×10^-4	1	5.523×10^-4	19.01	0.007 3	**
AC	4.000×10^-6	1	4.000×10^-6	0.14	0.725 8
BC	3.803×10^-4	1	3.803×10^-4	13.09	0.015 3	*
A²	5.688×10^-3	1	5.688×10^-3	195.81	<0.000 1	***
B²	4.523×10^-3	1	4.523×10^-3	155.70	<0.000 1	***
C²	1.442×10^-4	1	1.442×10^-4	4.96	0.076 3
残差	1.452×10^-4	5	2.905×10^-5
失拟项	3.125×10^-5	3	1.042×10^-5	0.18	0.900 2	不显著
净误差	1.140×10^-4	2	5.700×10^-5
合计	0.012	1
R²=0.987 4 R_adj²=0.964 8
信噪比=22.581 CV=2.0%

Microwave-assisted extraction technology of skin-whitening agent in white Poria cocos

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References 16

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试验号	因素			响应值/%
试验号	A	B	C	响应值/%
1	1	-1	0	24.0
2	0	-1	1	29.0
3	-1	-1	0	24.7
4	1	0	-1	26.5
5	0	1	-1	27.1
6	0	1	1	25.9
7	0	0	0	31.1
8	0	0	0	32.0
9	1	0	1	28.2
10	1	1	0	25.2
11	0	0	0	30.5
12	-1	0	1	26.6
13	-1	0	-1	25.3
14	0	-1	-1	26.3
15	-1	1	0	21.2

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