Abstract: To establish a new HPLC-FLD/DAD with series double-detector method for the determination of ten fluorescent whitening agents in masks.The sample was separated by Welch Ultimate LP C₁₈(4.6 mm×250 mm,3.5 μm) column with the gradient elution of methanol-0.05 mol/L ammonium dihydrogen phosphate solution as mobile phase at the flow rate of 1.0 mL/min.The column temperature was set at 35.0 ℃.The excitation wavelength and emission wavelength of fluorescence detector (FLD) were 360 and 430 nm,and the wavelength of diode array detector (DAD) was 360 nm.Results indicate that ten fluorescent whitening agents have good linear relationship in their linear range,with correlation coefficient greater than 0.999 5.At the three spiked levels,the average recoveries of ten fluorescent whitening agents are in the range of 87.6%-114.2% with the relative standard deviations (RSD,n=6) less than 5.0%.The detection limits of these ten fluorescent whitening agents are 0.026-0.40 mg/kg for FLD and 0.50-2.7 mg/kg for DAD.

Key words: masks, fluorescent whitening agents, high performance liquid chromatography