油茶籽油不皂化物的提取与抗氧化性研究

doi:10.13218/j.cnki.csdc.2018.06.006

Abstract

Abstract: Camellia oil was used as raw material to extract unsaponifiable matter (USM) by saponification-hexane extraction method.The compositions of USM in camellia oil were analyzed by gas chromatography-mass spectrometry (GC-MS),and DPPH radical-scavenging assay,ferric reducing-antioxidant power (FRAP) test and cellular antioxidant activity assay (CAA) were applied to determine their antioxidant activity in comparison with α-tocopherol.Results indicate that the compositions of USM in camellia oil obtained by saponification-hexane extraction are mainly hydrocarbon,triterpene alcohol and phytosterol.The DPPH free radical scavenging rate of USM in camellia oil is 95%,and the EC₅₀ is 1.9 g/L.USM in camellia oil has certain ability to reduce Fe³⁺,and the FRAP value is 170 at the mass concentration of 5 g/L.USM in camellia can enter the HSF cells and inhibit the ABAP-induced intracellular reactive oxygen species formation.The CAA value is up to 36.6,when the mass concentration of USM is 100 mg/L.

Key words: cosmetic raw material, camellia oil, unsaponifiable matter, antioxidation

CLC Number:

TQ658

ZHOU Zhen-yu, YANG Cheng, CAI Chun-hui. Study on extraction of unsaponifiable matter in camellia oil and its antioxidant activity[J].China Surfactant Detergent & Cosmetics, 2018, 48(6): 330-335.

References

[1] Kammeyer A,Luiten R M.Oxidation events and skin aging [J].Ageing Research Reviews,2015,21:16-29.
[2] Yang J M.Application and prospect of plant extracts in cosmetics [J].China Surfactant Detergent & Cosmetics,2013,43(4):313-316.
[3] Tan P X,Ye T,Liu X X,et al.Research advances in antioxidant composition of botanical extracts and their action mechanisms [J].Food Science,2010,31(15):288-292.
[4] Yang C,Liu X,Chen Z,et al.Comparison of oil content and fatty acid profile of ten new Camellia oleifera cultivars [J].Journal of Lipids,2016,1(1):1-6.
[5] Bo Y A,Song D H,Zhang F Q,et al.Comparison of the nutritional value of camellia oil and olive oil [J].China Oils and Fats,2008,33(3):39-41.
[6] Farhoosh R,Tavassoli-Kafrani M H,Sharif A.Antioxidant activity of the fractions separated from the unsaponifiable matter of bene hull oil [J].Food Chemistry,2011,126(2):583-589.
[7] Gelmini F,Ruscica M,Macchi C,et al.Unsaponifiable fraction of unripe fruits of Olea europaea.:an interesting source of anti-inflammatory
constituents [J].Planta Medica,2016,82(3):273-278.
[8] Wagemaker T A L,Campos P M B G M,Fernandes A S,et al.Unsaponifiable matter from oil of green coffee beans:cosmetic properties and safety evaluation [J].Drug Development and Industrial Pharmacy,2016,42(10):1695-1699.
[9] Fang Y D,Liu L Q.The pentacyclic triterpene alcohols and tetracyclic triterpene alcohols in unsaponifiable matters from camellia seed oil [J].Journal of the Chinese Cereals and Oils Association,1999,14(3):18-22.
[10] Xiao Y P.Tea seed oil and oil tea camellia seed oil unsaponifiables research [D].School of Food Science & Technology Nanchang University,2015.
[11] Nestle M.Diet,life-style,and mortality in China:a study of the characteristics of 65 Chinese counties [J].BioScience,1991,41(10):725-727.
[12] Chen L,Cao D,Ding M,et al.Extraction of squalene from soybean oil deodorizer distillate [J].China Oils and Fats,2014,39(9):67-70.
[13] Benzie I F F,Strain J J.The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”:the FRAP assay [J].Analytical Biochemistry,1996,239(1):70-76.
[14] Liu Y Q,Zhang K H,Wang Y L,et al.Comparison of experimental conditions of CCK-8 and MTS for human amniotic epithelial cells proliferation assay [J].Chinese Journal of Rehabilitation Theory and Practice,2012,18(9):827-830.
[15] Wolfe K L,Liu R H.Cellular antioxidant activity (CAA) assay for assessing antioxidants,foods,and dietary supplements [J].Journal of Agricultural and Food Chemistry,2007,55(22):8896-8907.
[16] Mao D B,Jia C X,Sun X L,et al.Analysis of squalene and Vitamin E in functional vegetable oils [J].Journal of the Chinese Cereals and Oils Association,2007,22(2):79-82.
[17] Tang F B,Shen D Y,Liu Y H,et al.Analysis of main chemical components in camellia oil and olive oil [J].Journal of the Chinese Cereals and Oils Association,2013,28(7):108-113.
[18] Xing C H,Li J W,Wang X G,et al.Determination of fatty acid composition and Vitamin E content of Camellia oleifera oil by chromatographic technique [J].Journal of Food Science and Biotechnology,2011,30(6):838-842.

Study on extraction of unsaponifiable matter in camellia oil and its antioxidant activity

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	Yaoyao Li. Study on the anti-aging and antioxidant effects of isosinensetin [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 313-319.
[2]	Tang Yin,Lv Xiaofan,Wang Ying,Wu Yani. Study on chemical components, antioxidant activity and cognitive improvement of borneol essential oil [J]. China Surfactant Detergent & Cosmetics, 2021, 51(11): 1095-1101.
[3]	Lu-jun LI,Zhi-hong WANG,Yan-ping HUO,Xiang WANG,Qiu-ling YANG,Mi-jun PENG. Study on surfactant-assisted ultrasonic extraction of total polyphenols from Eucommia ulmoides leaves and their antioxidant activity [J]. China Surfactant Detergent & Cosmetics, 2020, 50(3): 164-170.
[4]	GAO Si-yu,HUO Tong,LI Xue-tao,YAO Xue-min,LU Jun-cong,QUAN Qiang-hua. Study on the anti-aging efficacy of Sanqi extract [J]. China Surfactant Detergent & Cosmetics, 2020, 50(11): 783-787.
[5]	Tian-cheng GAO,Quan SUN,Na TANG,Ya-ru YANG. Research on the preparation of “phellodendron beauty salt” and its antioxidative and antibacterial properties [J]. China Surfactant Detergent & Cosmetics, 2019, 49(3): 183-186.
[6]	REN Hong-tao, ZHOU Bin. Analysis of volatile components and antioxidation activity evaluation of essential oil and aqueous extract from Pelargonium graveolens L′Herit. [J]. China Surfactant Detergent & Cosmetics, 2017, 47(8): 463-467.
[7]	DAI Yue, YU Xiao-bin. Screening of antioxidant bacteria and optimization of conditions for fermentation of Rhodiola rosea with mixed bacteria [J]. China Surfactant Detergent & Cosmetics, 2017, 47(7): 408-413.
[8]	XU Yuan-hong, XU Hu-jun. Synthesis and performance of a skin-whitening agent 4-(1-phenethyl)-1,3-benzenediol [J]. China Surfactant Detergent & Cosmetics, 2017, 47(3): 153-158.
[9]	WANG Yi-li, GU Fei-yan, LI Chen-chen, YU Ling. Antioxidation activity and application in cosmetics of extract from seed of Sophora japonica [J]. China Surfactant Detergent & Cosmetics, 2017, 47(3): 159-163.
[10]	SONG Xiao-jie, ZHOU Chun-xia, WU Yue. Reconstruction of 3D skin model and its application in evaluation the efficacy of cosmetic raw materials to be used as active ingredients [J]. China Surfactant Detergent & Cosmetics, 2017, 47(11): 633-636.
[11]	ZHAO Guan-hua, TONG Chang-qing, LI Wei, QU Min. Research on application of peptide from flesh of artificial raised Andrias davidianus in toothpaste [J]. China Surfactant Detergent & Cosmetics, 2017, 47(1): 36-39.
[12]	HUANG Li-sen,PANG Hai-yue,WU Hong-tan,YE Zi-jian,WANG Peng,WANG Gui-hong. Test for antioxidation and whitening activity of ethanol extract from four kinds of Chinese herbal medicine in vitro [J]. China Surfactant Detergent & Cosmetics, 2016, 46(6): 349-352.
[13]	FENG Bing, MA Lai-ji. Study of anti-aging efficacy of bolete at cell theory level [J]. China Surfactant Detergent & Cosmetics, 2015, 45(9): 509-512.
[14]	SU Xue-jun, QIAN Ming-yue, ZONG Chun-yan, ZHENG Lin. Optimization and performance test of broad-spectrum ultraviolet absorbent from natural plants [J]. China Surfactant Detergent & Cosmetics, 2015, 45(9): 513-517.
[15]	GUO Hui-ling, LI Chen-chen, YU Ling. Antioxidation activity of natural cocoa butter and its application in skin cream [J]. China Surfactant Detergent & Cosmetics, 2015, 45(10): 577-581.