化妆品中芦荟苷的测定及其稳定性的研究

doi:10.3969/j.issn.1001-1803.2019.03.012

Abstract

Abstract:

The effects of temperature, pH, extraction solvent and metallic ions on the stability of aloin in aloe extract were investigated by HPLC method. The content of aloin and its decomposition products in aloe cosmetics was determined by establishing a calibration curve. The samples were ultrasonically extracted by 5 mL 90% methanol solution at 30 ℃ for 20 minutes, separated on a C₁₈ column by gradient elution using methanol, acetonitrile and 0.05% phosphoric acid as the mobile phases. Then 7 samples of cosmetics labeled aloe components were randomly selected for testing at 356 nm. The results show that one of the seven selected samples of aloe cosmetics contained aloe-emodin and chrysophanol, and one toner contained chrysophanol. And aloin or aloin degradation products were not detected in the rest.

Key words: cosmetic additive, aloin, stability, HPLC

CLC Number:

TQ658

Qiang ZHANG,Jun CUI,Hua GU,Xiao-feng YU,Xiao-feng SHAN,Ge ZHU. Determination of aloin in cosmetics and its stability[J].China Surfactant Detergent & Cosmetics, 2019, 49(3): 199-202.

Figures/Tables 6

Tab. 1

Tab. 2

Fig. 1

Tab. 3

Fig. 1

Fig. 2

References 11

[1]	Zhang Shasha, Wang Fengshan, Shi Hui . et al. Study on antibacterial and anti-inflammatory activities of aloe extract[J]. Heilongjiang Science and Technology Information, 2014,23(1) : 119.
[2]	Ouyang Yuzhu, Shi Aihua, Li Youshe . et al. Anti-oxidation action of aloes extract in cosmetic creams[J]. China Surfactant Detergent & Cosmetics, 2008,38(1) : 32-34.
[3]	Chen Daiyi, Dong Pengcheng . Aloe’s humectant in cosmetic[J]. Journal of Shantou University, 2008,4(23) : 42-46.
[4]	Chang X L, Wang C H, Feng Y M , et al. Effects of heat treatments on the stabilities of polysaccharides substances and barbaloin in gel juice from Aloe vera Miller[J]. J Food Engineering, 2006,75:245-251. doi: 10.1016/j.jfoodeng.2005.04.026
[5]	Yan Zheng, Chen Shuangxi, Kou Zhengfu , et al. Study on the stability of aloin aqueous solution[J]. Journal of Henan University (Nature Science Editon) , 2010,40(3) : 287-290. doi: 10.3788/AOS20103012.3552
[6]	Wu Tingting, Wang Chao, Li Jie , et al. Determination of anthraquinone-compound in cosmetics by high performance liquid chromatography[J]. China Surfactant Detergent & Cosmetics, 2006,36(1) : 53-55. doi: 10.1007/s11434-006-2076-2
[7]	Yu Chenghua, Du Ruyun, Li Chao . et al. Determination of tree anthraquinone compounds in cosmetics by liquid chromatogrphy tandem mass spectrometry[J]. Flavour Fragrance Cosmetics, 2014 ( 4) : 42-44.
[8]	Yang Zhenghe . Physiological functions and efficacy of aloe compounds[J]. Animal Husbandry and Feed Science, 2012,33(7) : 8-9.
[9]	Wang Tao, Xia Qile, Chen Jianbing , et al. Research progress on extraction, isolation and bioactivity of active ingredients from aloes[J]. Food and Fermentation Industries Technology, 2012,48(4) : 8-9.
[10]	Chen Xinxia, Zhang Yuhang, Lin Ying , et al. Analysis of anthraquinones in different samples of aloe with HPLC-DAD and LC-MS/MS[J]. Natural Product Research and Development, 2009,21:817-821.
[11]	Li Guangdong. Formulation and preparation of practical chemical products[M]. Beijing: China Textile Press, 2011: 128-130.

化合物	线性范围/（mg·L^-1）	线性方程	相关系数	LOD/（μg·L^-1）
芦荟苷B	0.5~50	Y=15.19X+3.374 0	0.999 9	32
芦荟苷A	0.5~50	Y=21.11X+4.236 5	0.999 9	24
芦荟大黄素	0.5~50	Y=56.78X+6.980 5	1.000 0	9.7
大黄酚	0.5~50	Y=53.20X+15.84 6	1.000 0	8.8

待测物	样品中含量/（μg·g^-1）	加标量（μg·g^-1）	平均回收率/%	RSD/%
芦荟苷B	2 133	100	96.62	2.5
		500	98.88	2.2
		2 000	101.4	3.5
芦荟苷A	3 050	100	94.70	3.0
		500	98.23	2.3
		3 000	96.88	3.1
芦荟大黄素	198	50	105.0	1.8
		200	112.2	1.2
		400	110.7	3.3
大黄酚	94	50	105.7	1.9
		200	98.64	2.6
		400	104.9	3.7

金属元素	含量/（μg·g^-1）
金属元素	爽肤水	乳液	面霜	护手霜	防晒霜	BB霜
As	0.04	ND	ND	ND	1.3	0.23
Cr	0.76	0.06	0.39	0.88	ND	0.07
Cu	0.82	0.44	0.08	0.75	0.31	0.04
Fe	3.3	8.8	4.5	0.4	ND	35.6
Mn	6.7	0.05	7.9	0.84	ND	7.2
Pb	0.43	0.06	0.03	0.76	0.58	0.64

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Determination of aloin in cosmetics and its stability

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