Abstract: Ampelopsis grossedentata seed oil was extracted from Ampelopsis grossedentata seed as raw material by aqueous enzymatic method.Process conditions were identified by single factor experiment and response surface methodology.Results showed that the optimum extraction conditions of Ampelopsis grossedentata seed oil are as follows:ratio of solution to raw material (mL/g),12.6;dosage of neutral protease,1.4%;enzymolysis temperature,52.0 ℃;enzymolysis time,3.2 h.Extraction yield of Ampelopsis grossedentata seed oil reaches 10.30%.With protein isolated from soybean and malt dextrin as the wall materials,the microencapsulation conditions of Ampelopsis grossedentata seed oil were examined by orthogonal experiment.Results showed that the optimum microencapsulation conditions are as follows:mas fraction of wall material,13%;mass ratio of core material to wall material,1∶6;spray dryer inlet air temperature and outlet air temperature,125 and 62 ℃ respectively;homogenizing pressure,28 MPa.Under these conditions,microencapsulation ratio of Ampelopsis grossedentata seed oil achieves 92.3%.

Key words: Ampelopsis grossedentata seed oil, extraction, aqueous enzymatic method, microencapsulation