薰衣草花提取物对酪氨酸酶的抑制作用研究

doi:10.13218/j.cnki.csdc.2015.02.007

Abstract

Abstract: Dried flower of lavender was used as raw material and it was extracted with 70% (volume fraction) ethanol.The extract was concentrated to concrete and dispersed in water.Then the dispersion was further extracted with petroleum ether,chloroform,ethyl acetate and n-butanol successively.The extracts from the petroleum ether,chloroform,ethyl acetate and n-butanol as well as the water phase raffinate were assayed for tyrosinase inhibitory effects using L-DOPA as substrate.Results showed that the water extract demonstrates significant inhibitory effects on tyrosinase activity.The IC₅₀ value is 0.96 g/L.Kinetic study showed that the inhibitory mechanism of water-soluble extract is reversible,and the inhibitory type is the linear mixed-type inhibition.The inhibition constants of the free enzyme and the enzyme-substrate complex were 3.00 and 12.00 g/L,respectively.

Key words: whitening cosmetic additives, extract of lavender flower, tyrosinase, inhibition, kinetics

CLC Number:

TQ658

LUO Bi, LI Guo-zhu, WANG Li-jun, MENG Qing-yan, LU Ya-ling, LIU Wen-jie. Inhibitory effect of extract from lavender flower on the tyrosinase activity[J].China Surfactant Detergent & Cosmetics, 2015, 45(2): 90-93.

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Inhibitory effect of extract from lavender flower on the tyrosinase activity

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