绿原酸对酪氨酸酶活性影响及动力学研究

doi:10.13218/j.cnki.csdc.2015.03.008

Abstract

Abstract: The effect of chlorogenic acid on the activity of monophenolase and diphenolase of tyrosinase was tested.Experimental results showed that the activity of both monophenolase and diphenolase can be activated by chlorogenic acid.Chlorogenic acid mass concentration that can result in 50% activity increase (IC₅₀) is 10.5 mg/L for monophenolase and 1.1 g/L for diphenolase.Chlorogenic acid can eliminate the lagging time of the monophenolase.The activation kinetics of chlorogenic acid analyzed by Lineweaver-Burk plots demonstrated a competitive activation for the activation of diphenolase and the activation constant (K_I) is determined as 1.91 g/L.

Key words: tyrosinase, chlorogenic acid, activation, kinetics

CLC Number:

TQ658

GONG Sheng-zhao, ZHANG Kai, SUN Yong, CHEN Jia-ling. Kinetics and effect of chlorogenic acid on tyrosinase activity[J].China Surfactant Detergent & Cosmetics, 2015, 45(3): 157-160.

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Kinetics and effect of chlorogenic acid on tyrosinase activity

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