Abstract: A high performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of coumarin and other 14 compounds,including dicoumarol,4-hydroxycoumarin,7-methoxycoumarin,6-methylcoumarin,8-hydroxypsoralene,psoralen,angelicin,8-methoxypsoralen,5-methoxypsoralen,neobavaisoflavone,bavachin,trioxsalen,imperatorin and isoimperatorin.The samples were extracted with methanol under the help of supersonic wave and centrifuged at 10 000 r/min for 5 min.The supernatant was separated on a Phenomenex Luna C₁₈ (2) (250 mm×4.6 mm,5 μm) column by gradient elution with acetonitrile-0.1% acetic acid solution as mobile phase at a flow rate of 1.0 mL/min and column temperature of 30 ℃.The analysis of 15 compounds was performed by HPLC with diode array detector at the wavelength of 210,250,280 nm,respectively.Identification of 15 compounds was achieved by retention time,and quantification analysis was performed by the calibration curve method.The method shows a good linearity in the range of 0.1-10.0 mg/L with correlation coefficients higher than 0.999.The limits of detection (LODs) for the 15 compounds are 0.1-0.7 mg/kg.The mean recoveries at three spiked levels are in the range of 90.5%-106.4% with RSDs of 0.7%-3.4%(n=6).The method is accurate and simple,and is suitable for the determination of 15 compounds.

Key words: additive of cosmetics, high performance liquid chromatography, coumarin, Psoralea corylifolia