经表皮失水率测量影响因素剖析及标准化操作研究

doi:10.3969/j.issn.2097-2806.2025.06.005

Abstract

Abstract:

The transepidermal water loss （TEWL） of human skin is affected by many factors. Different test schemes were designed to systematically compare the test results of the cheek and inner forearm and other areas of healthy subjects, and explore the influence of measurement methods, environmental conditions, test site, instruments and equipment on TEWL test results. The results show that different test methods （including different measurement modes, interval time between two measurement, subject waiting time and accumulated multiple measurements） affect the reproducibility of the test results, and TEWL test results are significantly different under different conditions of environment temperature and humidity that skin surface contact, facial test site and day and night time. There is a strong correlation between the test results of different types of TEWL instruments, but the test stability is significantly different. In this study, various factors affecting the measurement of transepidermal water loss are systematically analyzed, and a standardized operation method is established to provide reliable and scientific technical support for accurate evaluation of the efficacy of cosmetics.

Key words: transepidermal water loss, influencing factors, efficacy evaluation, standardization

CLC Number:

TQ658

Fengling Chen, Yaru Wang, Rongzi Ye, Jiaen Liang, Jiaying Xie, Jianhua Tan. Analysis of the influencing factors and study on the standardized operation of transepidermal water loss measurement[J].China Surfactant Detergent & Cosmetics, 2025, 55(6): 715-722.

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References 15

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测量间隔时间/s	0	10	20	30
0	—	>0.05	>0.05	0.009^*
10	>0.05	—	>0.05	>0.05
20	>0.05	>0.05	—	>0.05
30	0.009^*	>0.05	>0.05	—

测量间隔时间/s	0	10	20	30
0	—	0.010^*	0.034^*	0.008^*
10	0.010^*	—	>0.05	>0.05
20	0.034^*	>0.05	—	>0.05
30	0.008^*	>0.05	>0.05	—

等待时间/min	0	20	30	40	60
0	—	0.248	0.003^*	<0.001^*	<0.001^*
20	0.248	—	>0.05	0.256	0.077
30	0.003^*	>0.05	—	>0.05	>0.05
40	<0.001^*	0.256	>0.05	—	>0.05
60	<0.001^*	0.077	>0.05	>0.05	—

受试者编号	未使用手臂托架TEWL结果/ （g/hm²）				使用手臂托架TEWL结果/ （g/hm²）
受试者编号	均值	标准差	RSD/%	RSD均值/%	均值	标准差	RSD/%	RSD均值/%
1	10.78	0.87	8.07	9.48	8.13	0.38	4.66	4.85
2	6.83	0.59	8.61		6.20	0.45	7.24
3	7.26	0.73	10.12		5.74	0.13	2.32
4	6.41	0.64	9.94		6.52	0.41	6.32
5	5.88	0.70	11.86		6.78	0.51	7.58
6	9.41	0.92	9.81		6.47	0.32	4.93
7	7.06	0.80	11.35		6.88	0.49	7.15
8	5.98	0.50	8.31		6.29	0.18	2.84
9	5.81	0.48	8.32		6.79	0.26	3.90
10	5.74	0.48	8.42		7.00	0.11	1.59

受试部位			例数	TEWL值/（g/hm²）均值±标准差
面颊	a	眼中垂直延长线与鼻翼平行延长线交点处	72	21.83±4.72
	b	颧骨顶端		19.66±4.16^*
	c	眼尾垂直延长线与鼻翼平行延长线交点		21.11±4.41

Analysis of the influencing factors and study on the standardized operation of transepidermal water loss measurement

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时间点	N	均值±标准差	秩和检验
时间点	N	均值±标准差	z值	P值
上午	60	8.53±2.12	-4.782	<0.001^*
下午	60	7.82±2.11	-4.782	<0.001^*

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