芹菜素和原花青素舒缓敏感肌肤的作用机制

doi:10.3969/j.issn.2097-2806.2025.05.008

Abstract

Abstract:

To investigate the mechanisms of apigenin （API） and proanthocyanidins （PC） in soothing sensitive skin （SS）, a mast cell degranulation model was established by stimulating RBL-2H3 cells with the calcium ionophore A23187. Based on the combinatorial experiments, it was found that when API and PC were combined at the molar ratios of 4∶1 and 2∶1, they exhibited the antagonistic effects on histamine release （combination index CI>1）; when they are combined at the molar ratios of 1∶1, 1∶2 or 1∶4, they showed the synergistic effects on histamine release （CI<1）. Among them, the combination of API and PC at a molar ratio of 1∶1 showed the better potent synergistic antihistamine release effect （CI=0.70）. Histamine is a hallmark of the mast cell degranulation, consequently, the combination of API and PC at a molar ratio of 1∶1 yields the better efficiency in inhibiting the mast cell degranulation with the lowest IC₅₀ value. Compared to the utilization of API or PC alone, the IC₅₀ value was reduced by 11.150 and 6.503 µmol/L, respectively. Compared to the positive control paeonol （PA）, the treatment with the combination significantly reduced the β-hex secretion, decreased the F-actin cytoskeleton rearrangement, and markedly suppressed the release of TNF-α, IL-4, and MCP-1. Further studies on the signaling pathways related to the mast cell degranulation indicated that the combination effectively inhibited the intracellular Ca²⁺ influx and significantly suppressed the phosphorylation of calmodulin-dependent protein kinase （CaMK） and phospholipase C/protein kinase C （PLC/PKC）. In summary, the combination of API and PC at a molar ratio of 1∶1 exhibited the better synergistic antagonistic effect on the histamine release, inhibited the mast cell degranulation model activation by reducing Ca²⁺ influx and inhibiting the activation of Ca²⁺/CaMK and PLC/PKC pathways, stabilized the cell membranes, regulated the inflammatory factor secretion, and exerted an effect in alleviating sensitive skin.

Key words: apigenin, proanthocyanidins, mast cell, sensitive skin, soothing

CLC Number:

TQ658

Laijiao Wei, Rongrong Deng, Shiqian Zheng, Luoxuan Li, Kezheng Wang, Zhibin Shen. Mechanism of the action of apigenin and proanthocyanidins in alleviating sensitive skin[J].China Surfactant Detergent & Cosmetics, 2025, 55(5): 601-610.

Figures/Tables 11

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n（API）∶ n（PC）	c（API+PC）/ （μmol/L）	IC₅₀/ （μmol/L）	CI value
4∶1	2, 4, 8, 16, 32	44.652	1.454
2∶1	2, 4, 8, 16, 32	38.834	1.293
1∶1	2, 4, 8, 16, 32	20.604	0.705
1∶2	2, 4, 8, 16, 32	24.458	0.858
1∶4	2, 4, 8, 16, 32	24.037	0.861

Experimental group	c/ （μmol/L）	IC₅₀/ （μmol/L）
API	2, 4, 8, 16, 32	31.754
PC	2, 4, 8, 16, 32	27.107

Mechanism of the action of apigenin and proanthocyanidins in alleviating sensitive skin

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