白花蛇舌草提取液对日光性皮炎小鼠的保护作用及机制研究

doi:10.3969/j.issn.2097-2806.2025.05.006

Abstract

Abstract:

The paper aimed to study the antioxidant and anti-inflammatory effects of Hedyotis diffusa extract on mice with ultraviolet B （UVB）-induced solar dermatitis. KM mice were subjected to UVB irradiation for 7 days, which were also treated with topical application of 1% and 2% Hedyotis diffusa extract. Hematoxylin-eosin （HE） staining, and picric acid-acidic magenta method （VG） staining were performed to observe the state of mouse skin. The levels of oxidative stress indicators NOS, NO, T-SOD, and inflammation indicators TNF-α, IL-6, and IL-1β were detected by biochemistry and immunohistochemistry, and the mRNA or protein levels of Nrf2, Keap1, HO-1, NQO1, GCLC, NF-κB, HMGB1 and p65 were detected by qRT-PCR and western blotting in skin tissues. Compared with the UVB group, the degree of skin damage in mice in the 1% and 2% Hedyotis diffusa extract groups is improved, with collagen fibers aligned, collagen density increased, NOS and NO levels decreased, T-SOD levels increased, TNF-α, IL-6, IL-1β expression levels decreased, mRNA expression levels of Nrf2, Keap1, HO-1, NQO1, and GCLC in skin tissue increased, NF-κB, HMGB1 mRNA expression levels decreased, the relative protein expression levels of Nrf2, Keap1, HO-1, NQO1, GCLC, and p65 in skin tissues increased, and the protein expression level of HMGB1 decreased. It can be seen that Hedyotis diffusa extract can ameliorate UVB-mediated solar dermatitis in mice, and its antioxidant and anti-inflammatory mechanism may be related to Nrf2 and NF-κB signaling pathway.

Key words: Hedyotis diffusa extract, ultraviolet B, solar dermatitis, antioxidant, anti-inflammatory

CLC Number:

TQ658

Yuewen Ma, Xiaojing Li, Xuefeng Huang, Xian Chen, Chunli Wang. Protective effect and mechanism of Hedyotis diffusa extract on mice with solar dermatitis[J].China Surfactant Detergent & Cosmetics, 2025, 55(5): 581-590.

Figures/Tables 13

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References 28

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引物名称	引物序列（5'-3'）	长度/ bp
GAPDH	上游5'-CCTCGTCCCGTAGACAAAATG-3'	133
GAPDH	下游5'-TGAGGTCAATGAAGGGGTCGT-3'	133
Nrf2	上游5'-TGTCTTAATACCGAAAACAAGCAGC-3'	139
Nrf2	下游5'-GACCACAGTTGCCCACTTCTTTT-3'	139
Keap1	上游5'-CAGATTGACAGCGTGGTTCG-3'	290
Keap1	下游5'-TGCGGGCAGTCGTATTTGA-3'	290
HO-1	上游5'-GCTAAGACCGCCTTCCTGCT-3'	105
HO-1	下游5'-ACGAAGTGACGCCATCTGTGA-3'	105
NQO1	上游5'-TATCACCACTGGGGGTAGCG-3'	153
NQO1	下游5'-GGAGTGTGGCCAATGCTGTAA-3'	153
GCLC	上游5'-CATCCTCCAGTTCCTGCACATC-3'	143
GCLC	下游5'-CATCGCCTCCATTCAGTAACAAC-3'	143
NF-κB	上游5'-CGAGTCTCCATGCAGCTACG-3'	212
NF-κB	下游5'-TTTCGGGTAGGCACAGCAATA-3'	212
HMGB1	上游5'-GGAGATCCTAAAAAGCCGAGAG-3'	143
HMGB1	下游5'-GTCTTCCACCTCTCTGAGCACTT-3'	143

峰号	保留时间/min	面积	高度	化合物名称
1	5.600	64 139	5 746	去乙酰基车叶草苷酸
2	14.469	124 701	12 145	京尼平苷酸
3	15.582	2 245 169	163 398	去乙酰车叶草酸甲酯
4	18.252	1 430 383	77 942	鸡屎藤次苷甲酯
5	23.612	91 434	3 215	车叶草苷酸
6	28.847	4 639 935	211 240	车叶草苷
总计		8 595 761	473 686

组别	数量/只
组别	-	+	++	+++
空白组	12	0	0	0
模型组	0	2	5	5
康复新组	8	2	2	0
白花蛇舌草1%组	9	2	1	0
白花蛇舌草2%组	10	2	0	0

	总NOS活力/（U/mg）	NO含量/（μmol/（g·prot））	T-SOD样本活力/（U/（mg·prot））
空白组	0.450±0.082	0.150±0.008	83.010±1.543
模型组	0.640±0.040^#	0.900±0.082^###	58.650±0.816^####
康复新组	0.550±0.040	0.250±0.041^***	41.550±0.820^****
白花蛇舌草1%组	0.610±0.035	0.200±0.016^***	62.090±0.408^**
白花蛇舌草2%组	0.330±0.041^**	0.230±0.024^***	68.670±2.449^**

	TNF-α/平均光密度	IL-6/平均光密度	IL-1β/平均光密度
空白组	0.134±0.002	0.101±0.004	0.110±0.005
模型组	0.176±0.004^###	0.119±0.007^#	0.136±0.005^##
康复新组	0.110±0.005^***	0.092±0.001^**	0.144±0.005
白花蛇舌草1%组	0.104±0.001^****	0.094±0.001^**	0.094±0.005^**
白花蛇舌草2%组	0.093±0.002^***	0.090±0.001^**	0.095±0.001^***

Protective effect and mechanism of Hedyotis diffusa extract on mice with solar dermatitis

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t/min	φ（流动相A）/%	φ（流动相B）/%
0.01	5	95
7	5	95
8	8	92
19	8	92
20	11	89
36	11	89
37	5	95
42	5	95