基于光损伤皮肤成纤维细胞模型的鱼胶原蛋白肽修复性能研究

doi:10.3969/j.issn.2097-2806.2024.11.012

Abstract

Abstract:

Fish collagen peptides （FCPs） represent a class of polypeptide mixtures endowed with diverse bioactive functions. Recent studies indicate that orally administered fish collagen peptides can effectively and safely impact the skin, exhibiting benefits such as antioxidation, inhibition of melanin formation, delayed aging, and resistance to photoaging. However, research comprehensively evaluating the efficacy of fish collagen peptides at the cellular level is relatively scarce. This study established a UVA-induced photodamaged human skin fibroblast model to investigate the impact of collagen peptides on the proliferation of human skin fibroblasts from both cellular morphology and viability perspectives. Additionally, alterations in hyaluronic acid, type Ⅰ collagen, elastin content, and total antioxidant capacity were assessed after treating cells with different samples. Through a comprehensive examination encompassing morphology, cell viability, and crucial metabolic substances, the photodamage repair effect of fish collagen peptides were comprehensively evaluated. This research provides experimental data support for oral collagen peptides in the field of skin photodamage repair, laying the groundwork for the development and application of novel functional foods and collagen products in the future.

Key words: fish collagen peptides （FCPs）, photodamage, human skin fibroblast, repair, antioxidation

CLC Number:

TQ658

Chunhong Pu, Rongchang Wang, Zhongjie Hua, Jiachan Zhang, Chao Chen. Study on the repair performance of fish collagen peptides based on the UVA induced fibroblast photodamage model[J].China Surfactant Detergent & Cosmetics, 2024, 54(11): 1375-1381.

Figures/Tables 6

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References 33

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项目名称（单位）	检测结果
灰分（g/100 g样品）	0.29
水分（g/100 g样品）	5.49
蛋白质（以干基计，g/100 g样品）	99.40

蛋白肽分子量范围/Da	峰面积百分比/ % （λ=220 nm）	数均分子量（Mn）	重均分子量（Mv）
>10 000	未检出	未检出	未检出
10 000~5 000	1.1	5 882	5 998
5 000~3 000	6.44	3 598	3 666
3 000~2 000	7.99	2 439	2 471
2 000~1 000	20.75	1 433	1 469
1 000~500	32.82	718	735
500~189	26.21	248	258
<189	4.69	—	—

Study on the repair performance of fish collagen peptides based on the UVA induced fibroblast photodamage model

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