磷脂基微乳液的制备及其负载白藜芦醇的性能研究

doi:10.3969/j.issn.2097-2806.2024.10.006

Abstract

Abstract:

An O/W microemulsion （ME） was prepared by compounding soybean phospholipids and alkylglycoside sodium hydroxypropyl sulfonate （APGSHS） as the emulsifiers in a 2∶1 ratio. The optimal formulation was determined by the ternary phase diagram method: 1, 2-hexanediol as the co-surfactant, dibutyl adipate as the oil phase, the mass ratio of the complex surfactant to the co-surfactant （K_m） of 1∶2, the mass fraction of dibutyl adipate in the system of 8.00%, and the amount of water added of 60.00%, and the concentration of inorganic salts in the water of 0.1 mol/L.Transmission electron microscopy show that the microemulsion is spherical, with oil droplets uniformly distributed, and the particle size is 15-25 nm. The maximum loading of the system for resveratrol is （11.55±0.14） mg/mL, which shows a good solubilization effect. At 1.00% resveratrol loading, the microemulsion shows good stabilization effect, with a retention rate of 77.13%±0.51% after 28 days of storage at 50 ℃, which is 19.21% higher than that of the unwrapped one, and a retention rate of 57.21%±0.32% after 32 h of storage at 365 nm UV illumination, which is 20.72% higher than that of the unwrapped one. The transdermal permeation amount of resveratrol in this system is up to （461.12±35.01）μg/cm² for 24 h, which has a good osmotic effect on insoluble polyphenolic compounds.

Key words: surfactant, microemulsion, phospholipid, proposed ternary phase diagram, resveratrol, stability

CLC Number:

TQ658

Baotong Jia, Kuan Chang. Preparation of phospholipid-based microemulsion and properties of loaded resveratrol[J].China Surfactant Detergent & Cosmetics, 2024, 54(10): 1191-1200.

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References 21

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物质	质量/g	质量分数/%
水相	6.000	60.00
大豆磷脂	0.800	8.00
APGSHS	0.400	4.00
1, 2-己二醇	2.400	24.00
己二酸二丁酯	0.300	3.00
白藜芦醇	0.100	1.00

物质	质量/g	质量分数/%
水	6.000	60.00
吐温-20	0.500	5.00
1, 2-己二醇	3.400	34.00
白藜芦醇	0.100	1.00

	0 d		30 d
	粒径	PDI	粒径	PDI
无盐	15.47 nm	0.209	15.65 nm	0.213
0.1 mol/L无机盐强度	17.90 nm	0.208	18.17 nm	0.205
0.2 mol/L无机盐强度	26.19 nm	0.197	26.24 nm	0.2

		365 nm				50 ℃
		0 mol/L	0.1 mol/L	0.2 mol/L	对照	0 mol/L	0.1 mol/L	0.2 mol/L	对照
L	储存前	16.99	17.46	16.57	18.98	16.83	17.16	16.51	19.09
	储存后	7.61	9.55	8.67	7.05	9.45	14.00	11.93	9.58
	ΔL	-9.38	-7.91	-7.90	-11.93	-7.38	-3.16	-4.58	-9.51
a	储存前	-1.41	-1.71	-1.82	-0.95	-1.47	-1.70	-1.51	-0.89
	储存后	0.41	2.65	2.28	0.11	2.31	1.81	2.23	2.15
	Δa	1.82	4.36	4.10	1.06	3.78	3.51	3.74	3.04
b	储存前	1.45	2.15	2.33	-1.19	1.75	1.88	2.14	-1.03
	储存后	-3.46	-1.07	-1.51	-3.28	-0.61	4.58	5.37	-1.55
	Δb	-4.91	-3.22	-3.84	-2.09	-2.36	2.70	3.23	-0.52
ΔE		10.74	9.59	9.69	12.16	8.62	5.44	6.74	10.00

Preparation of phospholipid-based microemulsion and properties of loaded resveratrol

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