经皮应用水飞蓟提取物的抗衰老作用评价

doi:10.3969/j.issn.2097-2806.2024.08.013

Abstract

Abstract:

The study aims to investigate the anti-aging effect of Silybum marianum extract （SME） on skin aging mice induced by D-galactose （D-gal）. The mice were divided into 6 groups. The mouse model of skin aging was induced by subcutaneous injection of D-gal （1 g/ （kg·d）） on the back for 6 weeks. Body weight, skin water content, skin oxidative stress index, HYP and HA levels of mice in each group were measured. HE staining was used to evaluate the skin injury. The relative mRNA expressions of collagen Ⅰ, collagen Ⅲ, MMP-1 and MMP-3 in skin were detected by RT-qPCR. The relative expressions of Nrf2 （nucleus）, Keap1 and HO-1 in skin were detected by Western blotting. The results show that percutaneous application of all three doses of SME increase the skin water content, alleviate the skin injury, increase the levels of SOD, CAT and GSH-Px, decrease the level of MDA, increased the levels of HYP and HA, increase the relative expression of collagen Ⅰ and collagen Ⅲ mRNA, decrease the relative expression of MMP-1 and MMP-3 mRNA, increase the relative expression of Nrf2 （nucleus） and HO-1 protein, and decrease the relative expression of Keap1 protein in D-gal-induced aging mice （P<0.05）. ML385 inhibits the activation of Nrf2 pathway and weakens the anti-aging effect of SME （P<0.05）. This study shows that percutaneous application of SME has a good anti-aging effect on skin aging mice induced by D-gal, and Nrf2 pathway mediates the anti-aging effect of SME. SME may be a kind of anti-aging daily skin care material with high development value.

Key words: Silybum marianum extract, percutaneous application, anti-aging, oxidative stress

CLC Number:

TQ658

Meiling He,Limin Fan. Evaluation of anti-aging effect of percutaneous application of Silybum marianum extract[J].China Surfactant Detergent & Cosmetics, 2024, 54(8): 981-987.

Figures/Tables 8

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References 22

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Gene	Primer sequence
collagen Ⅰ	F：5′-AAACGTAGTCGATGCTGATGC-3′
collagen Ⅰ	R：5′-AGCTGATGCTGATCGTAGTCGTA-3′
collagen Ⅲ	F：5′-ACGTAGTCGTAAGGTCGTGA-3′
collagen Ⅲ	R：5′-CTGATGCTGATGCTGATCGTAA-3′
MMP-1	F：5′-ATAGATTCATGCCAGAACCTGA-3′
MMP-1	R：5′-TGCCTTTGAAATAGCGGACT-3′
MMP-3	F：5′-AATCAGTTCTGGGCTATACGA-3′
MMP-3	R：5′-TCGATCTTCTTCACGGTTGC-3′
GAPDH	F：5′-CCTCGTCCCGTAGACAAAATG-3′
GAPDH	R：5′-TGAGGTCAATGAAGGGGTCGT-3′

Evaluation of anti-aging effect of percutaneous application of Silybum marianum extract

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