中药复方MHC-20的抗化脓性链球菌功效与作用机制探究

doi:10.3969/j.issn.2097-2806.2024.03.006

Abstract

Abstract:

The antimicrobial properties and mechanisms of a formula of Chinese medicinal plants （MHC-20） against Streptococcus pyogenes were studied. The MIC of MHC-20 against S. pyogenes was 31.25 μg/mL or 0.31% as measured by broth micro-or macro-dilution. MHC-20 was also found to have strong killing effects on Staphylococcus aureus, Escherichia coli and Candida albicans. The results of instantaneous bactericidal activity showed that MHC-20 was able to kill S. pyogenes completely in 5 min. It could also significantly reduce the cell surface hydrophobicity and inhibit the formation of biofilm. Cell membrane permeability test showed that MHC-20 could induce the increase of relative conductivity and alkaline phosphatase, as well as the protein leakage, in a concentration-dependent manner. The results of scanning electron microscopy further confirmed that MHC-20 had a strong destructive effect on cell morphology. Finally, a mouse model of cutaneous infection with S. pyogenes was established. It was shown that MHC-20 could promote wound healing and significantly inhibit the loading concentration of S. pyogenes （P<0.01）; 5% MHC-20 and 20% MHC-20 significantly decreased the expression of IL-6 in serum and tissue of the mice （P<0.001）. 20% MHC-20 significantly decreased the expression of leukocytes （P<0.01）. In conclusion, MHC-20 had the potential to be developed into a broad-spectrum antibiotic and an effective drug for cutaneous infection with S. pyogenes.

Key words: Streptococcus pyogenes, MHC-20, Chinese medicinal plant, mechanism, skin infection

CLC Number:

TQ658

Cuicui Hu, Daihong Zhou, Xinwan Chen, Jialing Zhong, Canquan Mao. Efficacies and mechanisms of a formula of Chinese medicinal plants （MHC-20） against Streptococcus pyogenes[J].China Surfactant Detergent & Cosmetics, 2024, 54(3): 282-289.

Figures/Tables 6

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References 19

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菌株名称	MIC值/%
化脓性链球菌	0.31
金黄色葡萄球菌	1.25
大肠杆菌	1.25
粪肠球菌	1.25
白色念珠菌	1.25
MRSA	2.50
铜绿假单胞菌	5.00

Efficacies and mechanisms of a formula of Chinese medicinal plants （MHC-20） against Streptococcus pyogenes

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