基于脂质组学的敏感性皮肤表面脂质差异分析

doi:10.3969/j.issn.2097-2806.2023.09.002

Abstract

Abstract:

This paper established a method for collecting, detecting, and analyzing the human facial skin surface lipids based on lipidomics technology for the study of lipid metabolic disorders in sensitive skin. Firstly, the young female volunteers were divided into the sensitive skin group and non-sensitive skin group by lactic acid stinging test, then the physiological indices were detected and the facial skin surface lipids were analyzed by the ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry （UPLC-Q-TOF-MS）. Subsequently, the differential components were screened by orthogonal partial least-squares discrimination analysis （OPLS-DA） combined with t test and identified via high-resolution MS/MS, and totally 29 differential lipids of triglyceride （TG） were assigned. Compared with the non-sensitive skin group, the levels of these differential TGs are significantly reduced in the sensitive skin group, and the relevant biological significance is explained. This study provides theoretical guidance and technical support for the exploration of the pathogenesis of sensitive skin as well as the research and development of related skin care products.

Key words: lipidomics, sensitive skin, differential lipids, triglyceride

CLC Number:

TQ658

Wan Zongyuan, Tong Wei, Zhou Chun, Hu Ping, Ouyang Sheng, Zhang Hongyang. Differential analysis of sensitive skin surface lipids based on lipidomics[J].China Surfactant Detergent & Cosmetics, 2023, 53(9): 999-1007.

Figures/Tables 10

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Tab. 1

Tab. 2

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Tab. 3

Identification results of differential lipids between the two groups （all exist in the form of [M+NH4]+ adduct ion）"

Time/min	Lipids	m/z	Characteristic fragment ions	VIP	P
11.58	TG （16:1/16:0/15:0）	808.739 4	535.471 6, 537.486 1, 549.486 9	5.75	4.5×10^-5
11.81	TG （18:1/16:1/16:0）	848.765 0	549.485 7, 575.502 9, 577.517 0	5.23	1.3×10^-4
11.36	TG （16:1/16:0/14:0）	794.722 8	521.456 8, 523.471 7, 549.486 9	4.72	2.0×10^-5
11.11	TG （16:1/15:0/14:0）	780.707 9	509.453 8, 521.454 7, 535.470 4	4.40	2.0×10^-5
11.71	TG （16:0/16:0/14:0）	796.739 8	523.472 8, 551.499 8	4.25	6.4×10^-3
11.46	TG （18:1/16:1/14:0）	820.727 1	521.456 9, 549.489 3, 575.505 7	4.21	1.2×10^-4
10.88	TG （16:1/14:0/14:0）	766.692 1	495.438 3, 521.453 5	3.77	7.8×10^-6
11.66	TG （18:1/16:1/15:0）	834.737 2	535.469 7, 563.502 6, 575.503 1	3.41	1.9×10^-5
11.00	TG （16:1/16:1/14:0）	792.689 8	521.455 3, 547.470 9	3.40	2.0×10^-5
11.23	TG （16:1/16:1/15:0）	806.712 3	535.472 7, 547.471 1	3.40	3.2×10^-6
11.10	TG （18:1/16:1/14:1）	818.714 1	519.438 6, 547.471 4, 575.505 7	2.74	1.5×10^-5
11.53	TG （18:1/16:1/16:1）	846.727 8	547.471 4, 575.502 9	2.72	1.2×10^-4
10.61	TG （16:1/14:0/13:0）	752.677 1	481.423 1, 507.440 3, 521.454 7	2.52	3.4×10^-6
11.31	TG （17:1/16:1/16:1）	832.706 5	547.472 1, 561.487 9	2.20	1.3×10^-5
10.73	TG （16:1/15:0/14:1）	778.644 0	507.440 8, 519.441 2, 535.470 1	2.20	2.4×10^-5
10.48	TG （16:1/14:1/14:0）	764.676 7	493.422 0, 519.440 1, 521.456 7	1.98	6.0×10^-6
12.15	TG （20:0/18:0/16:1）	906.849 8	577.529 0, 605.550 1, 635.601 7	1.93	6.6×10^-4
10.35	TG （16:1/14:0/12:0）	738.661 5	467.410 8, 493.424 5, 521.455 9	1.84	6.6×10^-6
10.60	TG （16:1/16:1/14:1）	790.692 0	519.441 0, 547.472 5	1.77	6.9×10^-6
10.85	TG （16:1/16:1/15:1）	804.707 9	533.455 7, 547.470 1	1.73	2.9×10^-6
12.18	TG （24:0/16:1/15:0）	920.872 2	535.479 3, 649.613 5, 661.612 6	1.59	3.4×10^-3
10.76	TG （16:0/14:0/12:0）	740.677 8	467.415 3, 495.437 5, 523.470 0	1.47	1.0×10^-4
11.25	TG （18:2/16:1/16:1）	844.738 8	547.472 2, 573.492 5	1.46	2.3×10^-5
12.03	TG （18:1/18:1/17:0）	890.816 5	591.535 0, 603.533 3	1.20	2.1×10^-4
11.93	TG （18:1/18:1/17:1）	888.799 6	589.519 0, 603.535 1	1.18	1.1×10^-4
12.23	TG （25:0/16:1/16:0）	948.780 6	549.486 2, 675.634 2, 677.643 4	1.17	1.5×10^-2
10.20	TG （16:1/14:1/13:0）	750.660 4	479.407 7, 507.439 0, 519.443 1	1.17	6.8×10^-6
12.21	TG （26:0/16:1/16:1）	960.902 0	547.468 5, 689.645 8	1.10	7.3×10^-3
10.43	TG （15:0/14:0/12:0）	726.661 3	467.407 5, 481.423 9, 509.457 7	1.02	1.0×10^-5

Tab. 3

Fig. 7

References 16

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Group	Sebum/（μg/cm³）	Water （a.u）	TEWL/ （g/h/m³）	pH
Non-sensitive skin	131.0±106.4	64.4±10.4	18.6±3.7	5.2±0.7
Sensitive skin	85.5± 93.0	63.5±11.1	18.9±3.4	5.6±0.5

Differential analysis of sensitive skin surface lipids based on lipidomics

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