茶多酚的经皮渗透特征及其对UVA致HSF氧化损伤的保护作用

doi:10.3969/j.issn.2097-2806.2023.09.006

Abstract

Abstract:

The aim was to study the transdermal penetration characteristics of tea polyphenols and explore the protective effect of tea polyphenols on UVA-induced oxidative damage in human skin fibroblasts （HSF）. Using the Franz diffusion cell with rat skin as a barrier, a well-performed detection method was established to detect the percutaneous penetration indexes of catechin, epicatechin, epicatechin gallate and epigallocatechin gallate in tea polyphenols by high performance liquid chromatography （HPLC）. The model of HSF damaged by UVA was established to explore the effect of tea polyphenols on the content of reactive oxygen species （ROS）, the level of malondialdehyde （MDA）, the enzymatic activity of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） in cells. The results show that the detection method has good specificity, precision and average recovery. The transdermal permeability of catechin and epicatechin increase gradually with the increase of time, and the infiltration rate of epicatechin is greater than that of catechin. However, the transdermal permeability of epicatechin galate and epigallocatechin galate can not be detected in the 24 h experiment, but the two substances remain more in the skin, indicating that the four substances in tea polyphenols can be applied to the skin well. Besides, tea polyphenols have significant protective effect on UVA-induced oxidative damage in HSF by reducing the content of ROS and the level of MDA, increasing the enzymatic activity of SOD and GSH-Px.

Key words: tea polyphenols, percutaneous penetration, ultraviolet A radiation, human skin fibroblasts, oxidative damage

CLC Number:

TQ658

Shen Yixin, Tang Lirong, Zhang Hui. Percutaneous permeation characteristics of tea polyphenols and its protective effect on human skin fibroblasts under the oxidative damage of ultraviolet A[J].China Surfactant Detergent & Cosmetics, 2023, 53(9): 1035-1043.

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References 26

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对照品	回归方程	r	线性范围/ （μg/mL）
儿茶素	y=6 498.2x+1 122.9	0.999 9	1~500
表儿茶素	y=7 213.9x-237.09	0.999 9	1~500
表儿茶素没食子酸酯	y=16 399x-2 703.4	0.999 9	1~500
表没食子儿茶素没食子酸酯	y=11 322x-869.78	0.999 9	1~500

组分	仪器精密度/%	样品稳定性/%	加样回收率/%
儿茶素	0.97	0.45	0.32
表儿茶素	0.77	2.63	0.53
表儿茶素没食子酸酯	0.42	0.48	0.27
表没食子儿茶素没食子酸酯	0.67	12.22	0.39

组分	稳态渗透速率方程	渗透速率/ （μg/ （cm²·h））	时滞/h
儿茶素	y=1.406x-10.88	1.406	7.74
表儿茶素	y=2.614 1x-19.352	2.614 1	7.40
表儿茶素没食子酸酯	—	—	—
表没食子儿茶素没食子酸酯	—	—	—

组分	扩散池残留量/ （μg/cm²）	皮内滞留量/ （μg/cm²）	经皮累积渗透量/ （μg/cm²）
儿茶素	54.58±21.07	12.35±5.20	22.80±5.96
表儿茶素	80.37±29.43	16.99±6.25	42.98±8.40
表儿茶素没食子酸酯	80.24±44.92	94.82±33.64	0.00±0.00
表没食子儿茶素没食子酸酯	215.62±134.63	233.54±89.92	0.00±0.00

Percutaneous permeation characteristics of tea polyphenols and its protective effect on human skin fibroblasts under the oxidative damage of ultraviolet A

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时间/min	φ（A）/%	φ（B）/%
0.00	95	5
5.00	70	30
11.00	82	18
25.00	50	50
28.00	95	5
30.00	95	5