Welcome to China Surfactant Detergent & Cosmetics, Today is

China Surfactant Detergent & Cosmetics ›› 2023, Vol. 53 ›› Issue (4): 423-429.doi: 10.3969/j.issn.1001-1803.2023.04.008

• Development and application • Previous Articles     Next Articles

The preparation process of three leguminous plant peptides and their activities

Zhang Huirong1,2,Guo Miaomiao1,2,Chen Chen1,2,Pan Qianyin1,2,Zhang Ying1,2,Li Li1,2,*()   

  1. 1. College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China
    2. Beijing Key Laboratory of Plants Resource Research and Development, Beijing 100048, China
  • Received:2022-05-11 Revised:2023-03-29 Online:2023-04-22 Published:2023-04-25
  • Contact: * Tel.: +86-13522268011, E-mail: lili2212@163.com.

Abstract:

In this study, the proteins of mung bean (Vigna radiata), sword bean (Canavalia gladiate) and rice bean (Vigna umbellata) were extracted by alkaline solubilization, acid deposition and ultrasound-assisted methods. The anti-oxidant ability of the three legume peptides was investigated by DPPH and ABTS radical scavenging assays, and the anti-inflammatory ability of the three legume peptides was investigated by COX-2 inhibition assay. The results show that the total protein content obtained from C. gladiate, V. radiata and V. umbellata is 89.82%, 79.93% and 75.76%, respectively. V. radiata and V. umbellata peptides are produced by stepwise hydrolysis with papain (8 000 U/g) and neutral protease (8 000 U/g). C. gladiate peptides are obtained by stepwise hydrolysis with papain (8 000 U/g) and acidic protease (8 000 U/g). The total peptide content of the peptide samples from V. umbellata, V. radiata and C. gladiate are 69.27%, 53.31% and 51.18%, respectively. The IC50 values for DPPH radical scavenging are 1.92, 0.92 and 0.60 g/L for V. radiata, C. gladiate and V. umbellate, respectively, and 0.22, 0.20 and 0.17 g/L for ABTS radical scavenging, respectively. The anti-oxidant capacity ranks as V. umbellata peptide>C. gladiate peptide> V. radiata peptide in order of strength. The peptides of V. umbellata, C. gladiate and V. radiata all show good inhibition of COX-2, with the COX-2 inhibition rates of 95.23%, 92.65% and 64.23% at 2.0 g/L, respectively.

Key words: plant-derived bioactive peptides, Vigna radiata, Canavalia gladiate, Vigna umbellata, anti-oxidant, anti-inflammatory

CLC Number: 

  • TQ658