Abstract:

According to the “Technical Specification for Safety of Cosmetics” （2015 edition）, the cosmetics are tested for microbial indicators. The contaminated bacteria are identified by malDI-TOF-MS and 16S rRNA sequencing technology, and the homology of the contaminated strains is compared and analyzed to investigate the microbial contamination of cosmetics in Guangxi. The results show that the homology of the same bacterial strain （Pluralibacter gergoviae） from three batches of mask of the same manufacturer is high after comparison and analysis. A total of 1 521 batches are qualified among 1 538 batches of cosmetics with the pass rate of 98.9% during 2018 to 2020. Among them, the qualified rate of aerobic bacterial count is 99.0%, the qualified rate of mold and yeast is 99.9%, the qualified rate of Pseudomonas aeruginosa is 99.9%, and the qualified rate of Staphylococcus aureus and heat-resistant coliform is 100.0%. The samples of contaminated microorganisms are mainly facial mask, skin care, toothpaste, hair care, and skin care products for infants. The unqualified samples are 10 batches of facial mask, 4 batches of skin care, 2 batches of toothpaste and 1 batch of hair care. The work indicates that the contaminated microorganisms are exceeded the standard in some cosmetics, and the conditioned pathogens above the standards （such as Burkholderia cepacia complex，BCC and Pluralibacter gergoviae） are detected in some samples. Although the qualified rate of microbial indicators in cosmetics is increasing year by year, microbial contamination still needs to be paid attention to.

Key words: cosmetics, contaminated microorganism, analysis, identification