高效液相色谱法测定防晒类化妆品中邻甲氨基苯甲酸甲酯

doi:10.3969/j.issn.1001-1803.2022.12.013

Abstract

Abstract:

A high performance liquid chromatography（HPLC） method was established for the determination of methyl methylanthranilate in sunscreen cosmetics. After extraction, sonication and filtration, the samples were separated by Welch Xtimate^® C₁₈ chromatographic column, and qualitative and quantitative analysis were carried out by HPLC with PDA detector. Under the experimental conditions, there is a good linear relationship between peak areas and methyl methylanthranilate concentrations with the r value is 0.999 6. The detection limit is 0.009%, and the limit of quantification is 0.029%. The average recoveries are in the range of 99.33%-111.5% and the relative standard deviations are in the range of 0.3%-3.0%. This accurate and exclusive method is suitable for the qualitative and quantitative analysis of methyl methylanthranilate in sunscreen cosmetics.

Key words: methyl methylanthranilate, sunscreen cosmetics, high performance liquid chromatography

CLC Number:

TQ658

Wu Zhishan,Jiang Chengjun,Yin Shuai,Huang Haiping,Zhang Wei. Determination of methyl methylanthranilate by high performance liquid chromatography in sunscreen cosmetics[J].China Surfactant Detergent & Cosmetics, 2022, 52(12): 1348-1352.

Figures/Tables 9

Tab. 1

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Tab. 2

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References 9

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时间/min	φ（A）/%	φ（B）/%	φ（C）/%
0	18	2	80
20	25	45	30
30	25	45	30
35	20	40	40
38	50	45	5
39.5	50	45	5
40	30	20	50
41	65	30	5
49	30	65	5
50	18	2	80
55	18	2	80

质量浓度/（μg/mL）	回收率-1/%	回收率-2/%	回收率-3/%	回收率-4/%	回收率-5/%	回收率-6/%	平均值/%	RSD/%
1.198 4	103.3	105.8	101.3	106.0	102.9	105.2	104.1	1.9
119.84	105.7	106.3	106.0	106.3	106.5	106.4	106.2	0.3

质量浓度/ （μg/mL）	Day-1/%	Day-2/%	Day-3/%	平均值/%	RSD/%
1.198 4	103.2	104.9	102.9	103.7	1.1
119.84	107.4	104.2	106.8	106.1	1.7

基质	回收率/%			RSD/%
基质	1.198 4 μg/mL	11.984 μg/mL	119.84 μg/mL	1.198 4 μg/mL	11.984 μg/mL	119.84 μg/mL
水剂类基质	100.6	103.4	105.7	1.6	0.3	0.8
霜剂类基质	103.6	106.1	105.2	2.5	0.8	1.9
乳液类基质	111.5	107.1	99.33	3.0	1.5	2.9

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Determination of methyl methylanthranilate by high performance liquid chromatography in sunscreen cosmetics

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