Abstract:

This study is to dissect the effect and mechanism of Aloe vera gel on anti-inflammation. The ear inflammation model of male Kunming （KM） mice was induced by phorbol ester （TPA）. The accumulation of neutrophils in the lateral line region of zebrafish embryos was tested using copper sulfate. The inhibitory effect of Aloe vera gel on the secretion of cytokines in RAW264.7 cells, including TNF-α and IL-6, was studied in lipopolysaccharide （LPS） - induced inflammation model. The minimum inhibitory concentration （MIC） and inhibitory rate of aloe gel against Staphylococcus aureus and Propionibacterium acnes were also studied via broth microdilution method and suspension quantification method, respectively. The model of ear inflammation is successfully established in male KM mice, and Aloe vera gel inhibits the ear swelling of mice at 1.5 g/mL （P<0.05）. Aloe vera gel significantly inhibits neutrophil aggregation in zebrafish embryos at 0.7 and 2.50 mg/mL （P<0.01）. Aloe vera gel significantly inhibits the secretion of TNF-α and IL-6 in RAW264.7 cells at 0.75, 1.50 and 3.00 mg/mL （P<0.01）. The MIC of aloe gel against Staphylococcus aureus and Propionibacterium acnes are 12.5 and 3.125 mg/mL, respectively. The inhibitory rate of aloe gel against Staphylococcus aureus and Propionibacterium acnes increases with the prolongation of the action time and concentration of Aloe vera gel. Aloe vera gel achieves anti-Inflammatory effect possibly through anti-inflammatory pathway of reducing neutrophil aggregation and inhibiting the production of inflammatory factors TNF-α and IL-6, as well as directly inhibiting Staphylococcus aureus and Propionibacterium acnes.

Key words: Aloe vera gel, anti-inflammation, antibacterial, RAW264.7 cells