Abstract:

This study aims to investigate the efficacy of the fermentation filtrate of Saccharomyces cerevisiae SC03 isolated from the rhizosphere soil of Gentiana scabra in Himalayan region on the human skin fibroblasts and keratinocytes. The results of this study also provide a reference for further promoting the application of microbial resources from the Himalayan region in the field of cosmetics. Soil yeast strains were isolated and screened from the rhizosphere soil samples collected from Nyingchi, Tibet, and the efficacy of the fermentation filtrate on the human skin fibroblasts and keratinocytes was explored. The results indicate that the genome of SC03 is very similar to that of the model yeast strain S. cerevisiae S288c, but also has special features. The fermentation filtrate of S. cerevisiae SC03 at a content of 0.5%-5% does not show cytotoxicity to human skin fibroblasts and keratinocytes. The fermentation filtrate of S. cerevisiae SC03 at a content of 1% significantly promotes the synthesis of Laminin332 in keratinocytes and inhibits the increase of ROS caused by UV irradiation, which is 81.16% and 22.17%, respectively. The fermentation filtrate of SC03 at a content of 2% significantly promotes DNA and ATP content of dermal fibroblasts and reduces cellular senescence-associated β-galactosidase positivity, which is 15.43%, 12.89%, and 15.40%, respectively. These results suggest that the rhizosphere soil yeast fermentation filtrate has an effect of anti-oxidant, anti-aging and skin nutrition, which has the potential for further development and utilization in cosmetics production.

Key words: Saccharomyces cerevisiae SC03, Gentiana scabra rhizosphere soil, fermentation filtrate, anti-oxidant, anti-aging