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China Surfactant Detergent & Cosmetics ›› 2022, Vol. 52 ›› Issue (9): 969-974.doi: 10.3969/j.issn.1001-1803.2022.09.008

• Development and application • Previous Articles     Next Articles

Protective effect of pilose antler extracts against UVB-induced skin photoaging in mice

Tang Jinghui1,Wang Yi2,Ma Shuhua2,Sun Yanan2,He Gaiying2,*(),He Zhongmei1,*()   

  1. 1. College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, Jilin 130118, China
    2. Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2021-12-01 Revised:2022-08-31 Online:2022-09-22 Published:2022-09-23
  • Contact: Gaiying He,Zhongmei He E-mail:gaiyinghe2012@163.com;heather78@126.com

Abstract:

Skin aging is an irreversible physiological process. Many factors, such as long-term UVB radiation, can accelerate skin aging and lead to a series of skin problems, whose symptoms include irregular pigmentation, wrinkles, dryness, loss of elasticity and capillary dilation. Herein, the protective effects of pilose antler extracts (PAEs) on skin photoaging induced by UVB in mice were investigated. The changes of epidermal thickness in photoaged skin were observed by H&E staining, while the changes of skin water content in photoaged mice were detected by a stratum corneum water content tester and a subcutaneous 0.05 mm water content tester. The fluorescence signal intensity of collagen fibers was detected in vivo by two-photon excitation fluorescence (TPEF) imaging technique, while the azimuth angle changes of collagen fibers were studied by liquid crystal polarized light imaging. RT-qPCR was used to analyze the expression of different types of collagen, matrix metalloproteinases and matrix metalloproteinase inhibitors. The results showed that, PAEs significantly inhibited UVB-induced hyperproliferation in mouse skin and increased the water content in the stratum corneum and deep layers of photoaged mouse skin. PAEs significantly increased the fluorescence signal intensity of dermal collagen fibers in photoaged mice, decreased the variability of collagen fiber azimuth, promoted the expression of dermal type I and III collagen mRNA in photoaged mice, and regulated MMP-1 and TIMP-2 mRNA expression. The experimental results showed that PAEs could significantly improve UVB-induced skin photoaging and have important applications in anti-aging products.

Key words: pilose antler extract, photoaging, collagen, matrix metalloproteinase

CLC Number: 

  • TQ658