天山雪莲多糖外用对特异性皮炎小鼠的改善作用研究

doi:10.3969/j.issn.1001-1803.2022.07.003

Abstract

Abstract:

The therapeutic effect and its possible mechanism of Saussurea involucrata polysaccharide on atopic dermatitis （AD） in mice were studied. The polysaccharide of Saussurea involucrata was obtained by water extraction and alcohol precipitation process. The content of crude polysaccharide was determined. The atopic dermatitis model in mice was established with topical 2, 4-dinitrofluorobenzene （DNFB）. 40 Mice were randomly divided into blank group, model group, prednisolone positive group, and low-dose and high-dose Saussurea involucrata polysaccharide groups （100 and 500 μg/mL, respectively）. The skin lesions of each group of mice were recorded; the transepidermal water loss of the mice was tested; the lesions were stained with hematoxylin-eosin to observe the pathological characteristics; the expression levels of IL-6, IL-4, IL-13, Ig E, TNF-α, NOD 1, TLR-2 and CRAMPS in the skin lesions were determined by ELISA method. The results show that the content of Saussurea involucrata polysaccharide prepared in this experiment is 28.23%. Saussurea involucrate polysaccharide could effectively alleviate the skin symptoms of mice with DNFB-induced atopic dermatitis. The transepidermal water loss and the thickness of the epidermal layer are both reduced. The high-dose group of Saussurea involucrata polysaccharide was selected for cytokine detection. Compared with the model group, the expression levels of Th2-related cytokines IL-6 （P<0.01）, IL-4 （P<0.01）, IL-13 （P<0.01） and Ig E in the skin of mice （P<0.01） are effectively reduced for the Saussurea involucrate polysaccharide group. Meanwhile, the Saussurea involucrate polysaccharide group regulates the cytokines of the innate immune pathway, significantly reduces the expression levels of TLR-2 （P<0.01）, NOD1 （P<0.01） and TNF-α （P<0.01）, and significantly increases the expression level of CRAMPS （P<0.05）. The present study shows that, through inhibiting the cytokines IL-4 and IL-13 in Th2 inflammatory response process in atopic dermatitis, and through regulating the secretion and release of the innate immunity regulator of TLR-2, the inflammatory response in AD model mice is thereby inhibited.

Key words: Saussurea involucrata （Kar. et Kir.） Sch.-Bip. polysaccharide, atopic dermatitis, immunomodulation, cytokine

CLC Number:

TQ658

Liu Guangrong,Gan Xiaoshuang,Tai Meiling,Han Ping,Akber Aisa Haji,Du Zhiyun. Study on the improvement effects of Saussurea involucrata polysaccharide on atopic dermatitis in mice by external application[J].China Surfactant Detergent & Cosmetics, 2022, 52(7): 704-709.

Figures/Tables 6

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References 20

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组别	皮损评分	经皮失水率/%
空白组	0^**	10.09±1.42^**
模型组	9.50±0.71	63.41±3.17
阳性波尼松龙组	6.00±0.71^**	43.64±5.39^*
天山雪莲多糖低剂量组	6.13±0.60^**	51.65±1.47^*
天山雪莲多糖高剂量组	5.75±0.66^**	49.11±1.65^*

组别	表皮厚度/μm
空白组	15.62±0.92^**
模型组	66.20±4.40
阳性波尼松龙组	45.05±2.16^**
天山雪莲多糖低剂量组	50.35±1.70^**
天山雪莲多糖高剂量组	41.72±1.74^**

组别	IL-6/（pg/mg）	IL-4/（pg/mg）	IL-13/（pg/mg）	Ig E/（pg/mg）
空白组	95.15±2.13^**	274.82±16.48^**	94.77±12.54^**	242.65±3.30^**
模型组	146.21±3.24	426.90±7.30	132.29±2.34	335.19±20.27
阳性波尼松龙组	100.73±2.71^**	331.31±13.15^**	106.73±7.81^*	245.83±9.14^**
天山雪莲多糖组	94.90±5.62^**	346.23±15.13^**	108.72±1.50^*	272.46±7.77^**

组别	TLR-2/（pg/mg）	NOD1/（pg/mg）	CRAMPS/（pg/mg）	TNF-α/（pg/mg）
空白组	12.88±0.79^**	219.01±8.58	111.65±13.43^*	829.12±111.67^**
模型组	17.77±1.16	234.54±5.98	86.13±4.04	1 344.13±80.25
阳性波尼松龙组	12.21±0.59^**	190.72±9.09^**	107.06±8.06^*	872.87±91.75^**
天山雪莲多糖组	11.00±0.90^**	174.93±5.61^**	97.68±0.98	1 067.82±101.04^**

Study on the improvement effects of Saussurea involucrata polysaccharide on atopic dermatitis in mice by external application

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