葡萄籽发酵液抗衰老及美白功效研究

doi:10.3969/j.issn.1001-1803.2022.05.013

Abstract

Abstract:

As the largest by-products of the grape processing industry, the development and utilization of grape seeds has become one of the urgent problems to be solved. Grape seeds are rich in active substances, which have the functions of anti-oxidation, anti-aging, anti-radiation and whitening. They can remove excessive oxygen free radicals produced by the body under oxidative stress conditions, and reduce the skin injury caused by external radiation and environmental pollution, which have a potential role in skin care. In this study, grape seeds were fermented using microbial fermentation technology with red wine yeast to obtain grape seed fermentation broth. The anti-aging model organism C.elegans was selected, and a mouse melanoma cell whitening model was established to explore the anti-aging and whitening effects of grape seeds from the cellular and molecular levels. Research results show that grape seed fermentation broth can effectively resist aging and inhibit melanin production, which has anti-aging and whitening effects. It also has good safety, and has the application potential as cosmetic functional raw materials. The by-products of grape processing have been reused, and its potential application value has been discovered, which is in line with the concept of green development.

Key words: grape seed, fermentation technology, C.elegans, anti-aging, whitening, safety evaluation

CLC Number:

TQ658

Wang Dongdong,Sun Qianru,Wang Ziwen,Fang Jiaxuan,Li Meng,Wang Changtao. Study on the anti-aging and whitening effects of grape seed fermentation broth[J].China Surfactant Detergent & Cosmetics, 2022, 52(5): 545-552.

Figures/Tables 16

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References 20

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基因	方向	引物
β-actin	F	CTG AAG CCC CAC TCA ATC CA
β-actin	R	GCC AAG TCA AGA CGG AGG AT
daf-2	F	GTA ATT GGA GGC CGT TCG CT
daf-2	R	CGT GGG CAC ATC AAT CCA GT
daf-16	F	GTA ATT GGA GGC CGT TCG CT
daf-16	R	CGT GGG CAC ATC AAT CCA GT
hsp-16.2	F	CGT CGA AGA GAA ATC TGC TGA A
hsp-16.2	R	TGC AGC GAA CAA TAC TGT AAT TTA TG
sod-3	F	GAC GAT CAA CCC CTG TCG AA
sod-3	R	TAC TGT TCT TCG GGG AAC GC

试剂	体积/μL
2×SuperReal PreMix Plus	10.0
F	0.3
R	0.3
50×ROX Reference Dye	0.25
cDNA	1.0
RNase-free ddH₂O	8.15

评分/分	分级	出血现象	凝血现象	出血现象
0	无	—	—	—
1	轻度	细小血管出血和少量出血	血管内（外）轻度凝血或CAM膜轻度浑浊	仅小血管融解
2	中度	小血管和大血管出血,并有明显量的血液流出	血管内（外）中度凝血或CAM膜中度浑浊	小血管和大血管融解
3	重度	几乎所有血管都出血,大量血液流出	血管内（外）重度凝血或CAM膜重度浑浊	大血管和全部血管树都融解

评分范围/分	刺激性分类
ES<6	无刺激性
6≤ES<12	轻度刺激性
12≤ES<15	中度刺激性
ES≥15	重度刺激性

ρ/（μg/mL）	线虫条数/条	平均寿命/d	最大寿命/d
对照组	150	19.24±0.96	26.31±1.32
1	150	19.88±0.99	30.43±1.52^*
4	150	20.24±1.01	28.72±1.44
8	150	21.64±1.08^*	32.28±1.61^**

Study on the anti-aging and whitening effects of grape seed fermentation broth

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组别	平均产卵量/个
组别	第1天	第2天	第3天	第4天	第5天	总计
对照组	49.31±6.18	123.56±9.22	85.72±10.53	18.16±5.45	4.92±1.27	281.67±17.25
样品组	47.18±9.67	117.64±18.18	92.73±9.74	12.23±4.65	5.67±1.69	275.45±20.16

组别	线虫数目/条	平均耐热时间/h	最长耐热时间/h
对照组	300	21.73±0.64	35.4±1.75
样品组	300	25.13±1.08^**	38.25±1.86

样品	出血类型	评分/分						ES/分
样品	出血类型	1	2	3	4	5	6	ES/分
阴性对照（0.9% NaCl溶液）	出血	0	0	0	0	0	0	0
	血管凝血	0	0	0	0	0	0
	血管溶解	0	0	0	0	0	0
阳性对照（0.1 mol/L NaOH）	出血	2	2	2	2	2	2	12
	血管凝血	2	1	1	1	2	1
	血管溶解	1	1	1	1	1	1
葡萄籽发酵液	出血	0	0	0	0	0	0	0
	血管凝血	0	0	0	0	0	0
	血管溶解	0	0	0	0	0	0

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