青皮提取物缓解皮肤敏感的作用机制研究

doi:10.3969/j.issn.1001-1803.2022.04.011

Abstract

Abstract:

Citrus reticulata （Tangerine） extract （CR） was prepared according to a proprietary process from immature citrus fruit and it was rich in flavonoids and alkaloids. To investigate the mechanism of CR in relieving skin sensitivity through transient receptor potential vanilloid 1 （TRPV1）, TRPV1 over-expression cells, prostaglandin receptor 1（EP1） over-expression cells and neuroblast cells were prepared. A comparison between normal cells and TRPV1 over-expression cells showed that, capsaicin （CAP） and phenoxyethanol could increase the expression of interleukin 8, chemokine MCP-1 and prostaglandin E2 （PGE-2） by activating TRPV1 and decrease the migration rate of wound healing. However, the results showed that CR could significantly inhibit the overexpression of cytokines, especially for PGE-2, and could also promote the migration rate of wound healing. Furthermore, the PGE-2-EP1 signaling pathway was studied, and it was found that 0.5% volume fraction of CR could significantly inhibit the activation of luciferase induced by PGE-2 through its receptor EP1. Finally, it was verified on neuroblast cells, and it was found that 0.06%-0.25% volume fraction of CR could inhibit the extracellular Ca²⁺ influx induced by the activation of TRPV1 caused by CAP and phenoxyethanol, and then inhibit the signal transduction in skin sensitivity or skin irritation, and finally alleviate clinical symptoms such as heat, itching and pain. Therefore, through the control of the activation of TRPV1, CR can inhibit the secretion of nociceptive cytokines and the downstream signal transduction, promote skin barrier repair, and alleviate skin irritation and sensitivity caused by the external stimuli.

Key words: Citrus reticulata extract, sensitive skin, skin barrier repair

CLC Number:

TQ658

Zhou Lidan,Lu Yina,Liu Qing. Mechanism of Citrus reticulata extract in relieving skin sensitivity[J].China Surfactant Detergent & Cosmetics, 2022, 52(4): 418-424.

Figures/Tables 9

Fig. 1

Fig. 2

Fig. 3

Tab. 1

Tab. 2

Fig. 4

Fig. 5

Fig. 6

Fig. 7

References 26

[1]	Bevan S, Quallo T, Andersson D A. TRPV 1[J]. Handb. Exp. Pharmaco., 2014, 222: 207-245.
[2]	Frias B, Merighi A. Capsaicin, nociception and pain[J]. Molecules, 2016, 21 (6) : 797. doi: 10.3390/molecules21060797
[3]	Moore C, Gupta R, Jordt S E, et al. Regulation of pain and itch by TRP channels[J]. Neuroscience Bulletin, 2018, 34 (1) : 120-142. doi: 10.1007/s12264-017-0200-8
[4]	Olivier G, Killian L’Herondelle, Nicolas L, et al. TRPV1 and TRPA1 in cutaneous neurogenic and chronic inflammation: pro-inflammatory response induced by their activation and their sensitization[J]. Protein Cell, 2017, 8 (9) : 644-661. doi: 10.1007/s13238-017-0395-5 pmid: 28364279
[5]	Adriana, Ehnis-Pérez, M D, et al. Relationship between transient receptor potential vanilloid-1 expression and the intensity of sensitive skin symptoms[J]. Journal of Cosmetic Dermatology, 2016, 15 (3) : 231-237. doi: 10.1111/jocd.12204 pmid: 26695734
[6]	Heber S, Ciotu C I, Hartner G, et al. TRPV1 antagonist BCTC inhibits pH 6.0-induced pain in human skin[J]. Pain, 2020, 161 (7) : 1532-1541. doi: 10.1097/j.pain.0000000000001848
[7]	Li D G, Yang D H, Schmaus G, et al. Inhibition of TRPV1 prevented skin irritancy induced by phenoxyethanol. A preliminary in vitro and in vivo study[J]. International Journal of Cosmetic Science, 2016, 39 (1) : 11-16. doi: 10.1111/ics.12340
[8]	Inhibition of TRPV 1 for the treatment of sensitive skin[J]. Experimental Dermatology, 2010, 19 (11) : 980-986. doi: 10.1111/j.1600-0625.2010.01122.x
[9]	Staender S. Sensitive skin - a global challenge with upcoming solutions[J]. Journal of the European Academy of Dermatology and Venereology: JEADV, 2016.
[10]	Caterina M, Pang Z. TRP channels in skin biology and pathophysiology[J]. Pharmaceuticals, 2016, 9 (4) : 77. doi: 10.3390/ph9040077
[11]	Talagas M, Misery L. Role of keratinocytes in sensitive skin[J]. Frontiers in Medicine, 2019, 6: 108. doi: 10.3389/fmed.2019.00108
[12]	Misery L, Staender, et al. Sensitive skin[J]. Journal of the European Academy of Dermatology & Venereology Jeadv, 2016, 30(Suppl. 1): 2-8.
[13]	Bujak J K, Kosmala D, Szopa I M, et al. Inflammation, cancer and immunity-implication of TRPV1 channel[J]. Frontiers in Oncology, 2019, 9: 1087. doi: 10.3389/fonc.2019.01087
[14]	Gouin O, L’Herondelle K, Lebonvallet N, et al. TRPV1 and TRPA1 in cutaneous neurogenic and chronic inflammation: pro-inflammatory response induced by their activation and their sensitization[J]. Protein Cell, 2017, 8 (9) : 644-661. doi: 10.1007/s13238-017-0395-5
[15]	Schneider A, Batisti A P, Turnes B L, et al. Anti-hyperalgesic properties of ethanolic crude extract from the peels of Citrus reticulata (Rutaceae)[J]. Anais da Academia Brasileira de Ciências, 2020, 92 (1) : 20180793.
[16]	Alghamdi S. Antinociceptive effect of the Citrus flavonoid eriocitrinon postoperative pain conditions[J]. Journal of Pain Research, 2020, 13: 805-815. doi: 10.2147/JPR.S250391 pmid: 32368133
[17]	Iannitti T, Cerbo A D, Loschi A R, et al. Repeated administration of a flavonoid-based formulated extract from citrus peels significantly reduces peripheral inflammation-induced pain in the rat[J]. Food Science & Nutrition, 2020, 8 (7) : 3173-3180.
[18]	Manchope M F, Casagrande R, Verri W A. Naringenin: an analgesic and anti-inflammatory citrus flavanone[J]. Oncotarget, 2016, 8 (3) : 3766-3767. doi: 10.18632/oncotarget.14084
[19]	Straub I, Mohr F, Stab J, et al. Citrus fruit and fabacea secondary metabolites potently and selectively block TRPM3[J]. British Journal of Pharmacology, 2013, 168 (8) : 1835-1850. doi: 10.1111/bph.12076 pmid: 23190005
[20]	Li S, Chen W, Chen S, et al. Construction of RHBDF2-shRNA lentiviral vector and establishment of stably transfected Neuro-2a-RHBDF2-shRNA cell line[J]. Journal of Jilin University (Medicine Edition), 2019, 45 (6) : 1224-1230.
[21]	Fan Y, Jie Z. Understand spiciness: mechanism of TRPV1 channel activation by capsaicin[J]. Protn & Cell, 2017, 8 (3) : 169-177.
[22]	Li D G, Yang D H, Schmaus G, et al. Inhibition of TRPV1 prevented skin irritancy induced by phenoxyethanol. A preliminary in vitro and in vivo study[J]. International Journal of Cosmetic Science, 2016, 39 (1) : 11-16. doi: 10.1111/ics.12340
[23]	Hohjoh H, Inazumi T, Tsuchiya S, et al. Prostanoid receptors and acute inflammation in skin[J]. Biochimie, 2014, 107: 78-81. doi: 10.1016/j.biochi.2014.08.010
[24]	Kawabata A. Prostaglandin E2 and pain: an update[J]. Biological & Pharmaceutical Bulletin, 2011, 34 (8) : 1170-1173.
[25]	Ma W, Li L, Xing S. PGE2/EP4 receptor and TRPV1 channel are involved in repeated restraint stress-induced prolongation of sensitization pain evoked by subsequent PGE2 challenge[J]. Brain Research, 2019, 1721: 146335. doi: 10.1016/j.brainres.2019.146335
[26]	Rohm B, Holik A K, Somoza M M, et al. Nonivamide, a capsaicin analog, increases dopamine and serotonin release in SH-SY5Y cells via a TRPV1-independent pathway[J]. Molecular Nutrition & Food Research, 2013, 57 (11) : 2008-2018.

表达量/ （pg/mL）	HaCaT细胞			HaCaT-TRPV1-OE细胞
表达量/ （pg/mL）	Control	20 μmol/L CAP	20 μmol/L CAP+0.125% CR	Control	20 μmol/L CAP	20 μmol/L CAP+0.125% CR	抑制率/%
IL-8	154.49	173.64	202.16	179.55	543.61	231.89^***	57.34
MCP-1	2 814.48	4 013.40	2 469.47^***	2 962.27	5 167.17	2 045.93^***	60.41
PGE-2	210.70	215.45	190.71	1 053.86	1 704.14	875.48^***	48.63

表达量/ （pg/mL）	HaCaT细胞			HaCaT-TRPV1-OE细胞
表达量/ （pg/mL）	Control	苯氧乙醇	苯氧乙醇+0.125% CR	Control	苯氧乙醇	苯氧乙醇+0.125% CR	抑制率/%
IL-8	160.81	124.41	142.61	192.22	322.99	237.40^**	26.50
MCP-1	3 025.53	3 158.08	1 887.68^***	3 301.88	4 359.68	2 451.50^***	43.77
PGE-2	75.81	140.47	112.73^*	797.95	927.08	599.52^***	35.33

Mechanism of Citrus reticulata extract in relieving skin sensitivity

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 9

References 26

Related Articles 10

Metrics

Comments

Recommended 10

[1]	Wu Bi, Xiaohong Pan, Xiaoqin Tu, Shuai Yin, Hui Sun. Analysis of the mechanism of anti-sensitive skin effect of cosmetic raw material Stephania tetrandra based on network pharmacology [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 305-312.
[2]	Wan Zongyuan, Tong Wei, Zhou Chun, Hu Ping, Ouyang Sheng, Zhang Hongyang. Differential analysis of sensitive skin surface lipids based on lipidomics [J]. China Surfactant Detergent & Cosmetics, 2023, 53(9): 999-1007.
[3]	Feng Faqing, Li Jing. Scientific evaluation methods for soothing cosmetics efficacy [J]. China Surfactant Detergent & Cosmetics, 2023, 53(7): 816-825.
[4]	Tan Qidan, Bi Yongxian, Liu Lei, Hu Xueqing, Dai Xiaoyan. Research status of cosmetic soothing efficacy evaluation [J]. China Surfactant Detergent & Cosmetics, 2023, 53(2): 193-201.
[5]	Zhao Yunshan,Jiang Yueming,Lu Wenjia,Jia Mingming,Qu Xin. Study on the skin soothing and skin repair effects of Pogostemon cablin leaf extract [J]. China Surfactant Detergent & Cosmetics, 2022, 52(8): 833-836.
[6]	Cheng Lin,Ding Meihua,Liu Qi,Zhao Hua. Assessment of sensitive skin condition [J]. China Surfactant Detergent & Cosmetics, 2022, 52(7): 778-784.
[7]	Chen Qingsheng,Meng Xiao,Liu Ting,Li Chuzhong,Zhou Zhaofang,Gong Shengzhao. Study on soothing and repair effects of “Franicshield” on sensitive skin [J]. China Surfactant Detergent & Cosmetics, 2021, 51(12): 1221-1227.
[8]	ZHAO Xiao-min,QU Xin. Sensitive skin and skin microbiome [J]. China Surfactant Detergent & Cosmetics, 2019, 49(5): 335-340.
[9]	WANG Huan, PAN Yao. Efficacy evaluation of cosmetics (Ⅴ) Scientific support for soothing skin efficacy claims [J]. China Surfactant Detergent & Cosmetics, 2018, 48(5): 247-254.
[10]	FAN Lin-na, JIA Yan, JIANG Li-gang, BI Yong-xian, DONG Yin-mao, HE Cong-fen. Brief analysis of causes for sensitive skin and progress on evaluation methods for anti-allergic activity of cosmetics [J]. China Surfactant Detergent & Cosmetics, 2015, 45(7): 409-414.