睡莲花提取物对酪氨酸酶的抑制作用及其动力学研究

doi:10.13218/j.cnki.csdc.2017.04.008

Abstract

Abstract: Tyrosinase inhibitory ratio of extracts from water lily (Nymphaea candida Presl.) flower (70% ethanol extract,NCE;30% ethanol elution parts,NCEA;50% ethanol elution parts,NCEB) were obtained based upon determination of dopa quinone with ultraviolet spectrophotometer.Furthermore,the effect of the extracts on monophenolase activity of tyrosinase and its inhibiting mechanism were examined by enzyme reaction kinetics method adopting L-tyrosine as substrate.Results show that these extracts can obviously inhibit activity of tyrosinase,of which NCEA displays the best inhibition effect as comparing with that of NCE and NCEB.The inhibition of NCEA on monophenolase activity of tyrosinase displays as reversible anti-competitive inhibition;while NCEB displays as reversible inhibition with mixed inhibition under low concentration condition,and competitive inhibition under high concentration condition.

Key words: additive for whitening cosmetics, extracts of water lily (Nymphaea candida Presl.) flower, tyrosinase, inhibition mechanism, kinetics

CLC Number:

TQ658

LI Qian, LI Chen-yang, ZHAN Yu-jiao, ABUDUREYIMU Maiwulajiang, XU Fang, ZHAO Jun. Inhibitory action of extracts from water lily flower on tyrosinase activity and its kinetics[J].China Surfactant Detergent & Cosmetics, 2017, 47(4): 219-222.

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Inhibitory action of extracts from water lily flower on tyrosinase activity and its kinetics

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