白梅花提取物抗氧化及美白功效评价

doi:10.3969/j.issn.1001-1803.2022.02.010

Abstract

Abstract:

In vitro evaluation method was used to study the anti-oxidation and whitening effects of Prunus mume extract. Preliminary evaluation of its antioxidant activity was conducted by measuring the IC₅₀ of Prunus mume extract on superoxide anion and hydroxyl radical scavenging. And in the H₂O₂-induced oxidative damage model of human skin fibroblast cell line （HFF-1 cells）, the antioxidant activity of Prunus mume extract was evaluated by examining the cell morphology, cell survival rate, malondialdehyde （MDA） content, reactive oxygen radical （ROS） content, superoxide dismutase （SOD） activity, glutathione （GSH） activity, type I collagen （ColⅠ） content and matrix metalloproteinase 1 （MMP-1） content. The tyrosinase activity inhibition experiment and the melanin content determination experiment of B16-F10 cells were used to evaluate the whitening effect of the Prunus mume extract. The results show that the Prunus mume extract has free radical scavenging effect, and the 60% and 90% alcohol extract of Prunus mume can significantly increase the activity of HFF-1 cells, SOD activity, GSH activity and Col Ⅰ content, and decrease the content of ROS, MDA and MMP-1. And it can also significantly reduce the melanin content in B16-F10 cells. To sum up, the white plum extract has obvious antioxidant and whitening effects.

Key words: Prunus mume, free radical, antioxidant, melanin, tyrosinase

CLC Number:

TQ658

Zha Yufeng,Huang Jiawen,Zhan Yi,Li Ting,Yan Hong,Wu Desong. Evaluation of antioxidant and whitening efficacy of Prunus mume extract[J].China Surfactant Detergent & Cosmetics, 2022, 52(2): 172-179.

Figures/Tables 9

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References 18

[1]	Zhang Cuili, Fu Lina, Yang Xiaoyun, et al. Research progress on the relationship between reactive oxygen free radicals and cell aging[J]. Guangzhou Chemical Industry, 2015, 43(19): 5-7.
[2]	Deng Ying, Zhong Jianqiao. Research progress on skin photodamage and oxidative stress[J]. Journal of Clinical Dermatology, 2015, 43(19): 5-7.
[3]	Tao Lili, Liu Yang, Wu Jinhao, et al. Research progress on evaluation methods of cosmetics whitening efficacy[J]. Detergent & Cosmetics, 2015, 38(3): 15-21.
[4]	Eugene S, Eunju S, Kyoung S C, et al. Inhibitory effect of Gastrodia elata Blume extract on alpha-melanocyte stimulating hormone-induced melanogenesis in murine B16F10 melanoma[J]. Nutr. Res. Pract., 2017, 11(3): 173-179. doi: 10.4162/nrp.2017.11.3.173 pmid: 28584573
[5]	Guo Haiyang, Mo Linlan, Tan Haisheng, et al. Study on the whitening effect and antibacterial and antioxidant activities of tea saponin[J]. Journal of the Chinese Cereals and Oils Association, 2020, 35(6): 83-89.
[6]	Yoshikawa M, Murakami T, Ishiwada T, et al. New flavonol oligoglycosides and polyacylated sucroses with inhibitory effects on aldose reductase and platelet aggregation from the flowers of Prunus mume[J]. J. Nat. Prod., 2002, 65(8): 1151-1155. pmid: 12193020
[7]	Chen Jing, Chen Mingzhu, Huang Xiangyu, et al. The antidepressant effect of Prunus mume extract on mice[J]. Strait Pharmaceutical Journal, 2016, 28(7): 20-22.
[8]	Chen Mingzhu, Chen Jing, Huang Youxia, et al. Study on the effect and mechanism of the total flavonoids of Prunus mume on the depressive behavior of chronic stress depression model rats[J]. China Pharmacy, 2017, 28(13): 1758-1762.
[9]	Hisashi M, Toshio M, Tomoko I, et al. Medicinal flowers.Ⅷ. radical scavenging constituents from the flowers of Prunus mume: structure of prunose Ⅲ[J]. Chem. Pharm. Bull., 2003, 51(4): 440-443. doi: 10.1248/cpb.51.440
[10]	Zhang Qinghua. Study on chemical constituents of Prunus mume and pre-test of biological activity[D]. Jinan: Shan Dong University, 2008.
[11]	Pi K, Lee K. Prunus mume extract exerts antioxidant activities and suppressive effect of melanogenesis under the stimulation by alpha-melanocyte stimulating hormone in B16-F10 melanoma cells[J]. Biosci. Biotechnol. Biochem., 2017, 81(10): 1883-1890. doi: 10.1080/09168451.2017.1365591
[12]	Wang Cancan, Zhang Wei, Wu Deling, et al. Research progress on chemical constituents and pharmacological effects of Prunus mume[J]. Guangzhou Chemical Industry, 2017, 45(24): 40-42, 72.
[13]	Wang Cancan, Zhang Wei, Wu Deling, et al. Study on the chemical constituents of the ethyl acetate parts of prunus mume[J]. China Journal of Chinese Materia Medica, 2020, 45(2):347-351. doi: 10.19540/j.cnki.cjcmm.20190828.201 pmid: 32237317
[14]	Tan Li, Chen Ruizhan, Chang Qingquan, et al. Process optimization and antioxidant activity of Epimedium leaf polysaccharide[J]. Food Science, 2017, 38(2): 255-263.
[15]	Zha Yufeng. Antioxidant activity of scutellarin and its aglycon derivatives, Caco-2 cell transport and blood-brain barrier permeability in vitro[D]. Guiyang: Guiyang Medical University, 2015.
[16]	WangJiaman, Lan Haimei. Application of collagen and its products in skin aging and skin repair[J]. Chinese Journal of Aesthetic Medicine, 2012, 21(1): 176-178.
[17]	Liu Yang, Deng Yingmei, Zhao Hua. Evaluation method of anti-wrinkle efficacy of cosmetics[J]. Detergent & Cosmetics, 2015, 38(4): 18-21.
[18]	Hyeong J P, Moon M K, Yunghee O. Effect of fruit extract of prunus mume on the scavenging activity of reactive oxygen species and melanin production in B16-F10 cells[J]. J. Life Sci., 2012, 22(7): 936-942. doi: 10.5352/JLS.2012.22.7.936

组别	IC₅₀/（mg/L）
组别	超氧阴离子	羟自由基
白梅花30%醇提物	376.4	268.5
白梅花60%醇提物	156.6	88.3
白梅花90%醇提物	96.7	67.5
阳性对照（维生素C）	11.9	18.4

组别	ROS/（IU/mL）	MDA含量/（nmol/mgprot）	SOD活力/（U/mgprot）	GSH活力/（μmol/mgprot）
空白对照组	2.65±0.17	1.42±0.41	29.22±0.99	85.06±3.83
模型组	3.51±0.16^#	2.89±0.36^#	15.28±2.15^#	56.60±2.18^#
白梅花30%醇提物	3.30±0.20	2.68±0.37	17.53±2.51	59.96±3.18
白梅花60%醇提物	3.09±0.18^*	2.26±0.34^*	20.15±3.16^*	67.31±4.86^*
白梅花90%醇提物	2.92±0.22^*	1.81±0.29^*	23.65±1.77^*	75.17±3.26^*

Evaluation of antioxidant and whitening efficacy of Prunus mume extract

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