大麻二酚的抗炎和抑菌活性研究

doi:10.3969/j.issn.1001-1803.2021.07.011

Abstract

Abstract:

CCK-8 assay was performed to evaluate the effect of cannabinol （CBD） on the relative cell activity of HaCat cells and RAW264.7 cells. The inhibitory effect of CBD on the secretion of inflammatory cytokines in HaCat cells and RAW264.7 cells induced by LPS was investigated. The inhibitory effect of CBD on anaerobe Propionibacterium acnes（P.acnes） was tested by the means of inhibition ring and inhibition rate. The results show that when the concentration is lower than 20 μmol/L, CBD presents no cytotoxicity to normal/inflammatory HaCat cells and normal/inflammatory RAW264.7 cells, nor does it promote the proliferation and differentiation of the cells. CBD shows a good inhibitory effect on TNF-α in inflammatory RAW264.7 cell model, and its effect is equivalent to the positive control dexamethasone （DEX） at the concentration of 10 μmol/L. CBD shows significant inhibitory effect on IL-6 in both inflammatory cell models. At the concentration of 20 μmol/L, it presents significant inhibitory effect on IL-1β in both cell models; and presents significant inhibitory effect on IFN-γ in both cell models at the concentrations of 10 and 20 μmol/L, all indicating its good anti-inflammatory activity. The CBD emulsion was applied toP.acnes, and the results show that when the concentration of CBD is above 20 μmol/L, it shows significant inhibitory effect on P.acnes. In addition, the results of bacteriostatic rate test show that when the concentration of CBD is 80 μmol/L, its antibacterial rate against P.acnes is as high as 99%, indicating that it has a good antibacterial effect.

Key words: cannabidiol, anti-inflammatory, Propionibacterium acnes, antibacterial

CLC Number:

TQ658

Liu Juan,Yu Siyi,Zhai Wenli,Li Guoqing,Li Xuetao,Liu Jitao. Investigation on the anti-inflammatory and antimicrobial activity of cannabidiol[J].China Surfactant Detergent & Cosmetics, 2021, 51(7): 655-661.

Figures/Tables 8

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References 21

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c（CBD）/（μmol·L^-1）	抑菌环直径/mm			x±s值
20	9.2	9.5	9.5	9.4 ±0.14
40	12.2	12.5	12.0	12.23±0.21
80	13.0	13.2	13.0	13.07±0.09
160	14.0	14.5	14.2	14.23±0.21

序号	对照组存活菌/（cfu·mL^-1）	对照组存活菌平均值/（cfu·mL^-1）	实验组存活菌/（cfu·mL^-1）	平均抑菌率/%
1	1.5×10⁴	2.5×10⁴	<10	>99.96
2	3.2×10⁴		<10
3	2.7×10⁴		<10

Investigation on the anti-inflammatory and antimicrobial activity of cannabidiol

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